Efficacy of colimox® (a new combination of amoxicillin and colistin) in the control of experimentally induced necrotic enteritis in broiler chickens

  • Authors

    • Mohamed Elbadawy Lecturer of Pharmacology Department, Faculty of Veterinary Medicine, Benha University
    • Mohamed Aboubakr Lecturer of Pharmacology Department, Faculty of Veterinary Medicine, Benha University
    2017-04-05
    https://doi.org/10.14419/ijpt.v5i1.7193
  • Amoxicillin, Colistin, Efficacy, C. Perfringens, Broiler Chickens.
  • The aim of the present study was to determine the efficacy of amoxicillin and colistin either alone or in combination (colimox®) for control of experimentally induced C. perfringens infections in broiler chickens. Three hundred birds were used and divided into five groups (A, B, C, D and E, each of 60 bird). At the 14th day of age, birds in all groups (except group A) were inoculated orally with 0.5 mL of C. perfringens broth culture (109 CFU/mL). Two days later, drugs were orally administered once daily for five consecutive days as follow; Group A and B were left untreated. Group C, D and E were treated with amoxicillin (20 mg/kg b.wt), colistin (100000 IU /kg b.wt), colimox® (amoxicillin plus colistin), respectively. The efficacy of used drugs were determined on the basis of clinical symptoms, mortality rate, body weight, total feed consumption, feed conversion rate and scores of intestinal lesions. The minimum inhibitory concentration (MIC) of amoxicillin against C. perfringens was done by micro-broth dilution method and was ≤ 2 µg/mL. Efficacy results indicated that all used medications were effective (but with different degrees) in control of C. perfringens induced necrotic enteritis as expressed by significant (p<0.05) reduction of clinical signs, mortalities and intestinal lesion scores as well as improving the performance parameters in broiler chickens, however colimox® was the superior. It could be concluded that combination of amoxicillin and colistin (colimox®) was of a considerable value in control of necrotic enteritis in broiler chickens than using them separately.

  • References

    1. [1] Abd El-Ghany WA (2010) Comparative evaluation on the effect of coccidiostats and synbiotic preparations on prevention of Clostridium perfringens in broiler chickens. Global Veterinaria 5, 324-333. https://idosi.org/gv/gv5(6)10/6.pdf.

      [2] Amin A, El-Ansary A & Issa Y (1994) Colorimetric determination of amoxicillin in pure form and in pharmaceutical preparations. Talanta 41, 691-694. https://doi.org/10.1016/0039-9140(94)80050-2.

      [3] Arroyo LA, Mateos I, González V & Aznar J (2009) In vitro activities of tigecycline, minocycline, and colistin-tigecycline combination against multi- and pan drug-resistant clinical isolates of Acinetobacter baumannii group. Antimicrobial Agents and Chemotherapy 53, 1295-1296. https://doi.org/10.1128/AAC.01097-08.

      [4] Aslam S, Durrani AZ, Rizwan M & Kashif M (2016) Comparative efficacy of metronidazole in combination colistin sulphate and probiotics against Clostridium perfringens and their effect on histopathological features of visceral organs in broiler. Veterinaria 4, 11-17. http://thesciencepublishers.com/veterinaria/files/4-v4i1-2016004.pdf.

      [5] Baba E, Ikemoto T, Fukata T, Sasai K, Arakawa A & McDougald LR (1997) Clostridial population and the intestinal lesions in chickens infected with Clostridium perfringens and Eimeria necatrix. Veterinary Microbiology 54, 301-308. https://doi.org/10.1016/S0378-1135(96)01289-8.

      [6] Barnes EM, Mead GC, Barnum DA & Harry EG (1972) the intestinal flora of the chicken in the period 2 to 6 weeks of age, with particular reference to the anaerobic bacteria. British Poultry Science 13, 311-326. https://doi.org/10.1080/00071667208415953.

      [7] Brady MS, Strobel JR & Katz SE (1988) In-vitro analytical system for determining the ability of antibiotics at residual levels to select for resistance in bacteria. Journal of the Association of Official Analytical Chemists 71, 295-298.

      [8] Brander G, Pugh D & Bywater R (1993) Veterinary Applied Pharmacology and Therapeutic. Bailliere, Tindall, 5th ed, pp. 55-63.

      [9] Brennan J, Moore G, Poe S, Vessie G, Barnum D & Wilson J (2001) Efficacy of infeed tylosin for the treatment of necrotic enteritis in broiler chickens. Poultry Science 80, 1451-1454. https://doi.org/10.1093/ps/80.10.1451.

      [10] Brennan J, Skinner J, Barnum DA & Wilson J (2003) the efficacy of bacitracin methylene disalicylate when fed in combination with narasin in the management of necrotic enteritis in broiler chickens. Poultry Science82, 360-363. https://doi.org/10.1093/ps/82.3.360.

      [11] Broussard CT, Hofacre CL, Page RR & Fletcher OJ (1986) Necrotic enteritis in cage reared commercial layer pullets. Avian Diseases 30, 617-619. http://www.jstor.org/stable/1590433 https://doi.org/10.2307/1590433.

      [12] Chakraborty GC, Chakraborty D, Bhattacharyya D, Bhattacharyya S, Goswami UN & Bhattacharyya HM (1984) Necrotic enteritis in poultry in West Bengal. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 5, 54-57.

      [13] Craven SE (2000) Colonization of the intestinal tract by Clostridium perfringens and fecal shedding in diet-stressed and unstressed broiler chickens. Poultry Science 79, 843-849. https://doi.org/10.1093/ps/79.6.843.

      [14] Dahiya JP, Hoehler D, Wilkie DC, Van Kessel AG & Drew MD (2005) Dietary glycine concentration affects intestinal Clostridium perfringens and Lactobacilli population in broiler chickens. Poultry Science84, 1875-1885. https://doi.org/10.1093/ps/84.12.1875.

      [15] Dhillon AS, Parimal R, Lauerman L, Schaberg D, Weber S, Bandli D & Weir F (2004) High mortality in egg layers as a result of necrotic enteritis. Avian Diseases 48, 675-680. http://www.aaapjournals.info/doi/abs/10.1637/7113?code=aaap-site&journalCode=avdi. https://doi.org/10.1637/7113.

      [16] Elemam A, Rahimian J & Doymaz M (2010) in vitro evaluation of antibiotic synergy for polymyxin B-resistant carbapenemase producing Klebsiella pneumoniae. Journal of Clinical Microbiology 48, 3558-3562. DOI: 10.1128/JCM.01106-10 http://jcm.asm.org/content/48/10/3558.

      [17] Keefe TJ (1977) Toxicology studies with amoxicillin. Veterinary Medicine and Small Animal Clinics 72, 739-743.

      [18] Gad W, Hauck R, Krüger M & Hafez HM (2011) Determination of antibiotic sensitivities of Clostridium perfringens isolates from commercial turkeys in Germany in vitro. Arch. Geflügelk 75, 80-83. http://www.european-poultry-science.com/Determination-of-antibiotic-sensitivities-of-span-classws-name-Clostridium-perfringensspan-isolates-from-commercial-turkeys-in-Germany-span-classws-name-in-vitrospansupa-,QUlEPTQyMjA2MjgmTUlEPTE2MTAxNA.html.

      [19] Gadzinski P & Julian R (1992) Necrotic enteritis in turkeys. Avian Diseases 36, 792-798. http://www.jstor.org/stable/1591787. https://doi.org/10.2307/1591787.

      [20] Gharaibeh S, Al Rifai R & Al-Majali A (2010) Molecular typing and antimicrobial susceptibility of Clostridium perfringens from broiler chickens. Anaerobe 16, 586-589. https://doi.org/10.1016/j.anaerobe.2010.10.004.

      [21] Gordon NC, Png K & Wareham DW (2010) Potent synergy and sustained bactericidal activity of a vancomycin-colistin combination versus multidrug-resistant strains of Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy 54, 5316-5322. http://aac.asm.org/content/54/12/5316. https://doi.org/10.1128/AAC.00922-10.

      [22] Gunderson BW, Ibrahim KH, Hovde LB, Fromm TL, Reed MD & Rotschafer JC (2003) Synergistic activity of colistin and ceftazidime against multi antibiotic-resistant Pseudomonas aeruginosa in an in vitro pharmacodynamic model. Antimicrobial Agents and Chemotherapy47, 905-909. http://aac.asm.org/content/47/3/905. https://doi.org/10.1128/AAC.47.3.905-909.2003.

      [23] Keyburn AL, Sheedy SA, Ford ME, Williamson MM, Awad MM, Rood JI & Moore RJ (2006) Alpha-toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens. Infection and Immunity 74, 6496-6500. http://iai.asm.org/content/74/11/6496 https://doi.org/10.1128/IAI.00806-06.

      [24] Koutoulis KC, Filioussisc PG & Athanasioud LV (2015) Pharmacokinetics and clinical assessment of amoxicillin for the control of necrotic enteritis in broiler-breeders under field conditions. Avian Biology Research 8, 89-96. http://ir.lib.uth.gr//handle/11615/29929. https://doi.org/10.3184/175815515X14292912200460.

      [25] Kwon YK, LeeY J & Mo I P (2004) an outbreak of necrotic enteritis in the ostrich farm in Korea. The Journal of Veterinary Medical Science 66, 1613-1615. https://doi.org/10.1292/jvms.66.1613.

      [26] Lanckriet A, Timbermont L, De Gussem M & Van Immerseel F (2010) the effect of commonly used antibiotics in a subclinical necrotic enteritis model. Avian Pathology 39, 63-68. https://doi.org/10.1080/03079450903505771.

      [27] Landman D, Bratu S, Alam M& Quale J (2005) citywide emergence of Pseudomonas aeruginosa strains with reduced susceptibilityto polymyxin. B. The Journal of Antimicrobial Chemotherapy 55, 954-957. https://doi.org/10.1093/jac/dki153.

      [28] Liang W, Liu XF, Huang J, Zhu DM, Li J & Zhang J (2011) Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients. BMC Infectious Diseases 27,11:109. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098177/. https://doi.org/10.1186/1471-2334-11-109.

      [29] Lovland A & Kaldhusdal M (1999) Liver lesions seen at slaughter as an indicator of necrotic enteritis in broiler flocks. FEMS Immunology and Medical Microbiology 24,345-351. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.1999.tb01304.x/full. https://doi.org/10.1016/S0928-8244(99)00052-8.

      [30] Lovland A, Kaldhusdal M, Redhead K,Skjerve E & Lillehaug A (2004) Maternal vaccination against subclinical necrotic enteritis in broilers. Avian Pathology33, 81-90. https://doi.org/10.1080/0379450310001636255.

      [31] Martel A, Devriese LA, Cauwerts K, De Gussem K, Decostere A & Haesebrouck F (2004) Susceptibility of Clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. Avian Pathology 33, 3-7. https://doi.org/10.1080/0307945031000163291.

      [32] McDevit R, Broker J, Acamovic T & Sparks N (2006) Necrotic enteritis; a continuing challenge for the poultry industry. World’s Poultry Science Journal 62:221-247. https://doi.org/10.1079/wps200593.

      [33] M'Sadeq SA, Wua S, Swicka RA & Chocta M (2015) Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide. Animal Nutrition1, 1-11. https://doi.org/10.1016/j.aninu.2015.02.004.

      [34] Nagaralli B, Seetharamappa J & Melwanki M (2002) Sensitive spectrophotometric methods for the determination of amoxicillin, and piroxicam in pure and pharmaceutical formulations. Journal of Pharmaceutical and Biomedical Analysis 29, 859-864. https://doi.org/10.1016/S0731-7085(02)00210-8.

      [35] Nation RL & Li J (2009) Colistin in the 21st century. Current Opinion in Infectious Diseases 22, 535-543. https://doi.org/10.1097/QCO.0b013e328332e672.

      [36] Nauerby B, Pedersen K, & Madsen M (2003) Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens. Veterinary Microbiology 94, 257-266. https://doi.org/10.1016/S0378-1135(03)00118-4.

      [37] Opengart K & Songer JG (2013) Necrotic Enteritis. In: Diseases of poultry (Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL & Nair V ed.), John Wiley & Sons Inc, Iowa, pp. 949-953.

      [38] Palmer G, Buswell J & Yeoman G (1976) Amoxycillin: new veterinary penicillin. The Veterinary Record 99, 84-85. https://doi.org/10.1136/vr.99.5.84.

      [39] Parish WE (1961) Necrotic enteritis in the fowl Gallus gallusdomesticus. I. Histopathology of the disease and isolation of a strain of Clostridium welchii. Journal of Comparative Pathology 71, 377-393. https://www.ncbi.nlm.nih.gov/pubmed/14483884. https://doi.org/10.1016/S0368-1742(61)80043-X.

      [40] Petrosillo N, Ioannidou E & Falagas ME (2008) Colistin monotherapy vs. combination therapy: evidence from microbiological, animal and clinical studies. Clinical Microbiology and Infection 14, 816-827. https://doi.org/10.1111/j.1469-0691.2008.02061.x.

      [41] Porter RE Jr (1998) Bacterial enteritides of poultry. Poultry Science 77, 1159-1165. https://doi.org/10.1093/ps/77.8.1159.

      [42] Prescott JF, Sivendra R & Barnum DA (1978) the use of bacitracin in the prevention and treatment of experimentally induced necrotic enteritis in the chicken. The Canadian Veterinary Journal 19,181-183. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1789416/

      [43] Riddell C & Kong XM (1992) the influence of diet on necrotic enteritis in broiler chickens. Avian Diseases 36, 499-503. http://www.jstor.org/stable/1591740. https://doi.org/10.2307/1591740.

      [44] Sameh M, Nasser A & Gehan Gad (2005) Efficacy of metronidazole, clindamycin and the probiotic in Clostridium perferingens infection in chickens. 4th Int. Sci. Conf. Masoura. Pp. 1393-1205.

      [45] Sasaki J, Goryo M & Okada K (2000) Cholangiohepatitis in chickens induced by bile duct ligations and inoculation of Clostridium perfringens. Avian Pathology 29, 405-410. https://doi.org/10.1080/030794500750047144.

      [46] Sasaki Y, Yamamoto K, Tamura Y & Takahashi T (2001) Tetracycline-resistance genes of Clostridium perfringens, Clostridium septicum and Clostridium sordellii isolated from cattle affected with malignant edema. Veterinary Microbiology 81, 61-69. https://doi.org/10.1016/S0378-1135(01)00402-3.

      [47] Silva R,Salvarani R, Assis N & Pires F (2009) Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens. Bra­zilian Journal of Microbiology 40, 262-264.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769726/

      [48] Skinner JT, Bauer S, Young V, Pauling G & Wilson J. (2010) An economic analysis of the impact of subclinical (mild) necrotic enteritis in broiler chickens. Avian Diseases 54, 1237-1240. http://www.jstor.org/stable/40983055. https://doi.org/10.1637/9399-052110-Reg.1.

      [49] Snedecor GW & Cochran WG (1980) Statistical Methods 7th Ed., Iowa State University Press, Ames, IA, pp. 39-63.

      [50] Souli M, Rekatsina PD, Chryssouli Z, Galani I, Giamarellou H & Kanellakopoulou K (2009) Does the activity of the combination of imipenem and colistin in vitro exceed the problem of resistance in metallo-β- lactamase-producing Klebsiella pneumoniae isolates?. Antimicrobial Agents and Chemotherapy 53, 2133-2135. http://aac.asm.org/content/53/5/2133. https://doi.org/10.1128/AAC.01271-08.

      [51] Sumano Lopez H & Gutierrez Olivera L (2010) Farmacología Clínica en aves comerciales, Fourth Ed. McGraw Hill, Mexico DF, Mexico, pp. 54-196.

      [52] Thompson DR, Parreira VR, Kulkarni RR & Prescott JF(2006) Live attenuated vaccine-based control of necrotic enteritis of broiler chickens. Veterinary Microbiology 113, 25-34. https://doi.org/10.1016/j.vetmic.2005.10.015.

      [53] Tsai SS & Tung MC (1981) an outbreak of necrotic enteritis in broiler chickens. Journal of Chinease Society of Veterinary Science 7, 13-17.

      [54] Van Immerseel F, Huyghebaert G, Haesebrouck F & Ducatelle R (2004) Clostridium perfringens in poultry: An emerging threat for animal and public health. Avian Pathology 33,537-549. https://doi.org/10.1080/03079450400013162.

      [55] Vissiennon T, Kroger H, Kohler T & Kliche R (2000) Effect of amoxicillin and ionophore on Clostridium perfringens entero-toxaemia in chickens. Berliner Mün. Tierarz. Woch., 113, 9-14.

      [56] Wagner D, Furrow R & Bradley B (1983) Subchronic toxicity of growth promoters in broiler chickens. Veterinary Pathology 20, 353-359. http://journals.sagepub.com/doi/abs/10.1177/030098588302000311. https://doi.org/10.1177/030098588302000311.

      [57] Watkins KL, Shryock R, Dearth N & Saif Y (1997) In-vitro antimicrobial susceptibility of Clostridium perfringens from commercial turkey and broiler chicken origin. Veterinary Microbiology 54, 195-200. https://doi.org/10.1016/S0378-1135(96)01276-X.

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    Elbadawy, M., & Aboubakr, M. (2017). Efficacy of colimox® (a new combination of amoxicillin and colistin) in the control of experimentally induced necrotic enteritis in broiler chickens. International Journal of Pharmacology and Toxicology, 5(1), 51-56. https://doi.org/10.14419/ijpt.v5i1.7193