Phytochemical Composition of Ethyl Acetate, Dichloromethane, n-Hexane ‎and Methanol Leaf Fractions of Gongronema Latifolium

Authors and Affiliations

  • Grace Ezinne Agu Department of Biochemistry, College of Natural Sciences, Michael Okpara University of ‎Agriculture, Umudike, P.M.B 7267, Umuahia, Abia State, Nigeria
  • Grace Effiong Department of Biochemistry, Faculty of Biological Sciences, University of Uyo, Uyo, Akwa Ibom ‎State, Nige-ria
  • Blessing Orji Department of Biochemistry, Faculty of Biological Sciences, University of Uyo, Uyo, Akwa Ibom ‎State, Nigeria
  • Ayokunle Michael Akinribido Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B 7267, Umuahia, Abia State, Nigeria https://orcid.org/0009-0002-3825-9950 (unauthenticated)

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Keywords:

Phytochemicals; Concentration; Alkaloid; Flavonoid; Antioxidant

Abstract

The phytochemicals present in ethyl acetate, dichloromethane, n-hexane, and methanol leaf fractions of Gongronema latifolium were identified using a phytochemical profiling method. The crude extract of Gongronema latifollium (990g) was dissolved in distilled water and partitioned successively and exhaustively with methanol, n-hexane, dichloromethane, and ethyl acetate using ‎a separating funnel. Alkaloids were present in high concentration (+++) in all four leaf extracts. ‎Flavonoids and terpenoids were observed to be present in high concentration (+++) except in n-‎hexane and ethyl acetate leaf extracts, respectively, which are moderately high concentration (++). ‎Cardiac glycoside was moderately high (++) in all four leaf extracts. From the results of this ‎study, it can be suggested that the fractions of Gongronema latifolium contain significant ‎phytochemicals in very high and moderately high concentrations, which may significantly alleviate ‎some health challenges.

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How to Cite

Agu, G. E., Effiong, G., Orji, B., & Akinribido, A. M. (2026). Phytochemical Composition of Ethyl Acetate, Dichloromethane, n-Hexane ‎and Methanol Leaf Fractions of Gongronema Latifolium. International Journal of Pharmacology and Toxicology, 14(1), 7-11. https://doi.org/10.14419/7j87ac58