Exposure to uppercott induces hepatotoxicity in male albino wistar rats

  • Authors

    • Emmanuel Modo Department of Biochemistry, Faculty of Science, Madonna University, Nigeria, Elele Campus, P.M.B. 48. Elele, Rivers State, Nigeria
    • Friday Uboh Department of Biochemistry, Faculty of Basic Medical Science, University of Calabar, Calabar, Rivers State, Nigeria
    • Margaret Agiang Department of Biochemistry, Faculty of Basic Medical Science, University of Calabar, Calabar, Rivers State, Nigeria
    • Efosa Ewere Department of Biochemistry, Faculty of Basic Medical Science, University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    2017-04-12
    https://doi.org/10.14419/ijsw.v5i1.7442
  • Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), Aspartate Transaminase (AST), Hepatotoxicity, Uppercott Pesticide.
  • Background: The use of herbicides and pesticides in agriculture and for public health purposes has led to harmful effects in many non-target species including man.

    Methods: In this study, the hepatic effect of exposure to cypermethrin and dimethoate mixture (uppercott) was investigated in male albino wistar rats. Forty five (45) male wistar albino rats were distributed into five (5) groups of nine (9) animals each. Groups 1 and 2 served as the normal control and oil control respectively, groups 3, 4 and 5 received 2.5%, 5% and 7.5% LD50 of uppercott orally for 28 days.

    Results: Results obtained revealed that uppercott exposure significantly (p<0.05) increased serum aspartate aminotransferase (AST) activity, alanine aminotransferase (ALT) activity and alkaline phosphatase (ALP) activity when the test groups were compared with control. Also, uppercott exposure raised serum bilirubin concentration slightly insignificantly compared to the control. Superoxide dismutase activity was significantly reduced across all test groups as compared with compared with control. Histology of liver tissues revealed patchy necrotic sessions in the liver tissues of the test experimental groups (2.5%, 5% and 7.5%).

    Conclusion: The results obtained from this study are strongly indicative of the hepatotoxic effect of uppercott pesticide and hence, caution during usage is advised.

  • References

    1. [1] United States Environmental Protection Agency. What is a pesticide? http://www.epa.gov/pesticides 2007. Accessed November 18, 2014.

      [2] R. Carolyn, H. Winand, C. Edward, H. Colleen, National Pesticide Applicator Certification Core Manual. National Association of State Departments of Agriculture Research Foundation, Washington, DC 2013.

      [3] R.K Stoelting, Liver and digestive tract. In R. K. Stoelting (Ed.), Pharmacology and physiology in anesthetic practice. 2nd edition. Philadelphia, J. B. Lippicott. 1991; 2: 782 – 794.

      [4] E. Hodgson, S.A. Meyer, Pesticides In: Hepatic and Gastrointestinal Toxicology, Permagon/Elsevier, Comprehensive Toxicology (1997) 9: 369-387.

      [5] M. Treinen-Moslen, Toxic responses of the liver. In Casarett and Doull's Toxicology: The Basic Science of Poisons, Klassen, C., Ed., McGraw-Hill, New York, 2001; 471-489.

      [6] S.A. Meyer, Overview of hepatotoxicity in: current protocols in toxicology (Maines, M.D., ed), John Wiley & Sons, New York 2001. https://doi.org/10.1002/0471140856.tx1401s08.

      [7] D. Lorke, A new approach to practical acute toxicity testing. Archive of Toxicology (1983) 54 (4):275-87. https://doi.org/10.1007/BF01234480.

      [8] L. Jendrassik, P. Grof, Colorimetric method of determination of bilirubin. Biochem. Z (1938) 297:81-82.

      [9] W. Thefeld, H. Hoffmeister, E.W. Busch, P.U. Koller, J. Vollmar, Reference values for the determination of GOT, GPT and alkaliphosphatase in serum with optimal standard methods. Dtsch. Med. W ochenschr. (1974) 99:343 - 351. https://doi.org/10.1055/s-0028-1107760.

      [10] H. Schlebusch, W. Rick, H. Lang, M. Knedal, Standards in the activities of clinically important enzymes. Dtsch Med Inochenschr, (1974) 99, 765 - 766. https://doi.org/10.1055/s-0028-1107840.

      [11] F. Paoletti, D. Aldinucci, A. Mocali, A. Caparrini, A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Analytical Biochemistry (1986) 154: 538–541. https://doi.org/10.1016/0003-2697(86)90026-6.

      [12] H. Jaeschke, G.J. Gores, A.I. Cederbaum, J.A Hinson, D. Pessayre, J.J. Lemasters, Mechanisms of hepatotoxicity. Toxicological Sciences (2002) 65:166–176. https://doi.org/10.1093/toxsci/65.2.166.

      [13] G. Lum, S.R. Gambino, Serum gamma glutamyltranspepti-dase activity as an indicator of disease of liver, pancreas, or bone. Clinical Chemistry (1972) 18(4):358-362.

      [14] A. Halim, O. El-Almady, S. Hassab-Allah, F. Abdel-Gelil, Y. Hafez, A. Derivis, Biochemical effect of antioxidants on lipids and liver function in experimentally induced liver damage. Annals of Clinical Biochemistry (1997) 34:656-663. https://doi.org/10.1177/000456329703400610.

      [15] E.O. Udosen, A.S. Ojong, Hepatotoxic activity of Sacoglothis gabonensis in rats. Pharmaceutical Biology (1998) 36(5):467-469. https://doi.org/10.1076/phbi.36.5.368.4648.

      [16] M.A. Crook, Clinical chemistry and metabolic medicine (7th ed.). New Delhi: Edward Arnold 2006.

      [17] O. Bodansky, Enzymes in cancer: The phosphohydrolases. In: Biochemistry of human cancer. Bodansky O. (Ed.), New York: Academic Press. 1995; 61-92.

      [18] F.E. Uboh, M.I. Akpanabiatu, U.E. Eyong, P.E. Ebong, O.O. Eka, Evaluation of toxicological implications of inhalation exposure to kerosene fumes and petrol fumes in rats. Acta Biologica Szegediensis (2005) 49 (3-4):19-22

      [19] E.G. Ewere, S.A. Oyebadejo, V.C. Peter, Ethanolic Leaf Extract of Irvingia gabonensis (O’ Rorke) Baill Protects against Nephrotoxicity and Hepatotoxocity in Cadmium-induced Wistar Albino Rats. International Journal of Pharmacology and Toxicology (2016) 4(2): 105-110. https://doi.org/10.14419/ijpt.v4i2.6186.

      [20] M. Fahimul-Haq, S. Mahmood, N. Choudhry, T. Shabaz, S. Akram, R. Yasmin, Study of effect of pesticides on total bilirubin and direct bilirubin levels in blood of workers of pesticide formulation & packing plants in Pakistan: Multiplexing with ALP, ALT and AST. Pakistan Journal of Medical and Health Sciences (2013) 7(3):736-739.

      [21] M.L. Queiroz, C. Binovoletto, R.C. Perlingeriro, M.R. Quadros, Immunoglobin levels in workers exposed to hexa-chlorobenzene. Human Experimental Toxicology (1998) 17 (3): 172-75. https://doi.org/10.1191/096032798678908477.

      [22] E.J. Brower, C.T. Evelo, A.J. Verplanke, R.T. Van Welie, F.A. De Wolff, Biological effect monitoring of occupational exposure to 1,3-dichloropropane: effect on liver and renal function and on glutathione conjugation. British Journal of Industrial Medicine (1991) 48(9): 646-648.

      [23] I.F. Usoh, E.J. Akpan, E.O. Etim, E.O. Farombi, Antioxidant actions of dried flower extracts of Hibiscus sabdariffa L. on sodium arsenite-induced oxidative stress in rats. Pakistani Journal of Nutrition (2005) 4: 135-141. https://doi.org/10.3923/pjn.2005.135.141.

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  • How to Cite

    Modo, E., Uboh, F., Agiang, M., & Ewere, E. (2017). Exposure to uppercott induces hepatotoxicity in male albino wistar rats. International Journal of Scientific World, 5(1), 74-79. https://doi.org/10.14419/ijsw.v5i1.7442