Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

  • Authors

    • Hatil EL-Kamali Omdurman Islamic University, Faculty of Science and Technology, Botany Department
    • Sana E. M. Hamed
    2015-06-08
    https://doi.org/10.14419/ijsw.v3i2.4741
  • Antioxidant Activity, Free Radical, Polar Extracts, Sudanese Flora.

  • In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of Punica granatum showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of Commiphora myrrha 58±0.01 and Azadirachta indica 57±0.20 respectively.

  • References

    1. [1] Chakraborty, P., Kumar, S., Dutta,D., Gupta,V.(2009).Role of Antioxidants in Common Health Diseases. Research J. Pharm. and Tech.2 (2):238-244.

      [2] Staners, S.A., Hughes,T., Kelly,N., Buttriss, J,A.(2004).Review of the Quideriological Evidence of the Antioxidants Hypothesis. Publ. Health Nutr.7:407-422.

      [3] Tiwari, P and Patel, R. K. (2011). Total Phenolics and Flavonoids and Antioxidant Potential of Draksharishta Prepared by Traditional and Modern Methods. Pharmacologyonline 3: 1072-1082.

      [4] Murthy, K.N., Reddy, V.K., Veigas, J.M., Murthy, U.D. (2004). Study on Wound Healing Activity of Punica granatumPeel.J Med Food. 7 (2):256-9.http://dx.doi.org/10.1089/1096620041224111.

      [5] Vijayasanthi,M., Kannan,V., Venkataswamy,R., Doss, A. (2012). Evaluation of the Antibacterial Potential of Various Solvent Extracts of Acacia niloticalinn. Leaves. Hygeia.J.D.Med. 4 (1):91-96.

      [6] Rangsriwong, P., Rangkadilok, N., Satayavivad, J., Gotoc, M., Shotipruk, A.(2009). Subcritical Water Extraction of Polyphenolic Compounds from Terminalia chebulaRetz. Fruits. Separation and Purification Technology. 66:51-56.http://dx.doi.org/10.1016/j.seppur.2008.11.023.

      [7] El-Ashmawy, I.M., Ashry, K.M., El-Nahas, A.F., Salama, O.M. (2006).Protection by Turmeric and Myrrh against Liver Oxidative Damage and Genotoxicity Induced by Lead Acetate in Mice. Basic Clinical and Pharmacology and Toxicology. 98 (1):32-7.http://dx.doi.org/10.1111/j.1742-7843.2006.pto_228.x.

      [8] Auffray B. (2007).Protection against Singlet Oxygen, the Main Actor of Sebum Squalene Peroxidation during Sun Exposure, Using Commiphora myrrha EssentialOil.International Journal of Cosmetic Science. 29(1):23-9.http://dx.doi.org/10.1111/j.1467-2494.2007.00360.x.

      [9] Aboul-Enein, A. M., Abu El-Ela, F., Shalaby, E.A., El-Shemy, H.A. (2012). Traditional Medicinal Plants Research in Egypt:Studies of Antioxidant and Anticancer Activities. Journal of Medicinal Plants Research.6 (5):689-703.

      [10] El-Kar, C., Ferchichi, A., Attia, F., Bouajila, J. (2011). Pomegranate (Punica granatum) Juices: Chemical Composition, Micronutrient Cations, and Antioxidant Capacity. Journal of Food Science.76(6):795-800.http://dx.doi.org/10.1111/j.1750-3841.2011.02211.x.

      [11] Orak, H. H., Demirci, A. Ş., Gümüş, T.(2011).Antibacterial and Antifungal Activity of Pomegranate (Punicagranatum L.cv.) Peel. EJEAFChe, 10 (3):1958-1969.

      [12] Ardekania M. RS., Hajimahmoodia, M., Oveisib, M. R., Sadeghib, N., Jannatc, B., Ranjbara, A. M., Gholamb, N., Moridib, T.(2011). Comparative Antioxidant Activity and Total Flavonoid Content of Persian Pomegranate (Punica granatumL.) Cultivars. Iranian Journal of Pharma- ceutical Research.10 (3):519-524.

      [13] Akbarpour, V., Hemmati, Kh,Sharifani, M.(2009).Physical and Chemical Properties of Pomegranate (Punica granatumL.) Fruit in Maturation Stage.American-Eurasian Journal of Agriculture and Environmental Sciences. 6(4): 411-416.

      [14] Reddy,M. K., Gupta, S. K., Jacob, M. R., Khan, Sh. I., Ferreira, D.(2007).Antioxidant, Antimalarial and Antimicrobial Activities of Tannin-Rich Fractions, Ellagitannins and Phenolic Acids from Punica granatum L. J Planta Med. 73(5):461-467.http://dx.doi.org/10.1055/s-2007-967167.

      [15] Ajaikumar, K.B., Asheef, M., Babu, B.H., Padikkala, J.(2004).The Inhibition of Gastric Mucosal Injury by Punica granatum L. (pomegranate) Methanolic Extract. Journal of Ethnopharmacology. 96(1):171-176.

      [16] Koko, W. S., Osman, E. E., Galal, M. (2009). Antioxidant and Antiglycation Potential of Some Sudanese Medicinal Plants. BoletínLatinoamericano y del Caribe de Plant as Medicinales y Aromáticas. 8(5):402 - 411.

      [17] Vadivel, V. and Biesalski, H.K.(2012).Total Phenolic Content, In vitro Antioxidant Activity and type II Diabetes Relevant Enzyme Inhibition Properties of Methanolic Extract of Iraditionally Processed Underutilized Food Legume, Acacia nilotica(L.) Willd ex. Delile. Food Research Journal 19.(2): 593-601.

      [18] Ali, A., Akhtar, N., Khan, B. A., Khan, M. S., Rasul, A., UZ-Zaman, Sh., Khalid, N., Waseem, Kh., Mahmood, T., Ali, L. (2012). Acacia nilotica: A Plant of Multipurpose Medicinal uses .Journal of Medicinal Plants Research. 6 (9):1492-1496.

      [19] Diwan, R., Shinde, A., Malpathak, N. (2012).Phytochemical Composition and Antioxidant Potential of Rutagraveolens L. In Vitro Culture Lines. Journal of Botany. 2012:1-6.http://dx.doi.org/10.1155/2012/685427.

      [20] Ratheesh, M., Shyni, G.L., Helen, A. (2009). Methanolic Extract of Ruta graveolens L. Inhibits Inflammation and Oxidative Stress in Adjuvant Induced Model of Arthritis in Rats. Inflammopharmacology. 17(2):100-105.http://dx.doi.org/10.1007/s10787-009-8044-0.

      [21] Chang ,C. L. and Lin, C. S.(2011).Phytochemical Composition, Antioxidant Activity, and Neuroprotective Effect of Terminalia chebulaRetzius Extracts. Evidence-Based Complementary and Alternative Medicine .2012:1-7.

      [22] Hazra, B., Sakar, R., Biswas, S., Mandal, N.(2010).Comparative Study of the Antioxidant and Reactive Oxygen Species Scavenging Properties in the Extracts of the Fruits of Terminalia chebula, Terminalia belerica, and Emblica officinalis.BMC Complementary and Alternative Medicine.10-20 http://www.biomedcentral.com/1472-6882/10/20.http://dx.doi.org/10.1186/1472-6882-10-20.

      [23] Mahesh,R., Bhuvana,Sh., Begum,V. M.( 2009). Effect of Terminalia chebula Aqueous Extract on Oxidative Stress and Antioxidant Status in the Liver and Kidney of Young and Aged Rats. Cell Biochem Funct.27:358-363.http://dx.doi.org/10.1002/cbf.1581.

      [24] Krishnamoorthy, P.1., Vaithinathan, S., Vimal Rani, A. Bhuvaneswari, A.(2007).Effect of Terminalia chebulaFruit Extract on Lipid Peroxidation and Antioxidative System of Testis of Albino Rats. African Journal of Biotechnology. 6 (16):1888-1891.

      [25] Bhattacharya, S., Chaudhuri, S.R., Chattopadhyay S., Bandyopadhyay, S. K.(2007).Healing Properties of Some Indian Medicinal Plants against Indomethacin-Induced Gastric Ulceration of Rats. Journal of Clinical Biochemistry and Nutrition. 41:106-114.http://dx.doi.org/10.3164/jcbn.2007015.

      [26] Suchalatha,S and Devi, C.S. (2007).Antioxidants Activity of Ethanolic Extract of Terminalia chebula Fruit against Isoproterenol - Induced Oxidative Stress in Rats. Indian Journal of Biochemistry and Biophysics. 42:246-249.

      [27] Cheng, H. Y., Lin, T.C., Yu, K.H., Yang, C.M., Lin, C.C. (2003). Antioxidant and Free Radical Scavenging Activities of Terminalia chebula. Journal of Biology and Pharmacy. Bull. 26 (9):1331-1335.

      [28] Lee, H.S., Won,N. H., Kim,K. H., Lee,H., Jun, W., Lee, K.W. (2005).Antioxidant Effects of Aqueous Extract of Terminalia chebulaInVivoandIn Vitro. Biol. Pharm. Bull. 28(9):1639-1644.http://dx.doi.org/10.1248/bpb.28.1639.

      [29] Dhanalakshmi,S., Devi,R.S.,Srikumar, R.,Manikandan, S., Thangaraj. R. (2007). Protective Effect of Triphala on Cold Stress- Induced Behavioral and Biochemical Abnormalities in Rats. Pharmaceutical Society of Japan.127 (11): 1863-1867.

      [30] Rahman, M. S., Begum, B., Chowdhury, R., Rahman, K. M., Rashid, M. A.(2008).Preliminary Cytotoxicity Screening of Some Medicinal Plants of Bangladesh. Journal of. Pharmacy. Sci.7 (1):47-52.

      [31] Rahmatullah,M., Islam,T., Hasan, E., Ahmed,R., Jamal, F., Jahan, R., Khatun, A., Nahar, N., Ahsan, S., Nahar,A., Ahmad, I. (2010).A survey of Medicinal Plants used by the Folk Medicinal Practitioners of Shetabganjvillage in Dinajpur District, Bangladesh. American-Eurasian Journal of Sustainable Agriculture.4 (2):196-203.

      [32] Saravanan,S., Srikumar,R., Manikandan, S., Parthasarathy, N.J., Devi, R.(2007).Hypolipidemic Effect of Triphala in Expermentally Induced Hypercholeseremic Rats. Pharmaceutical Society of Japan.127 (2):385-388.

      [33] Gundidza ,M., Gweru,N., Magwa,M. L., Ramalivhana, N. J., Humphrey, G., Samie, A., Mmbengwa, V. (2009).Phytochemical Composition and Bio- logical Activities of Essential Oil of Rhynchosia minima (L) (DC) (Fabaceae). African Journal of Biotechnology. 8 (5):721-724.

      [34] Kiendrebeogo, M., Dijoux-Franca, M.G., Lamien,C. E. Meda,A., Wouessidjewe, D., Nacoulma, O.G. (2005) .Acute Toxicity and Antioxidant Property of Striga hermonthica(Del.) Benth (Scrophulariaceae). African Journal of Biotechnology. 4 (9):919-922.

      [35] Behera,B. C., Verma, N., Sonone, A., Makhija,U. (2009). Optimization of Culture Conditions for Lichen Usnea ghattensis G. Awasthi to Increase Biomass and Antioxidant Metabolite Production.Food Technology and Biotechnology.47(1):7-12.

      [36] Heng, L., Yamamoto, Y., Liu, Y., Jung, J. S., Kahng, H. Y., Koh, Y. J., Hur, J.S.(2010).The In Vitro Antioxidant Properties of Chinese Highland Lichens. Journal of Microbiology and Biotechnology.20 (11):1524-1528.http://dx.doi.org/10.4014/jmb.1003.03029.

      [37] Agar, G., Aslan, A., Sarioglu, E. K., Alpsoy, L., Ceker, S. (2011).Protective Activity of the Methanol Extract of Usnea longissimaagainst Oxidative Damage and Genotoxicity Caused by Aflatoxin B1In vitro. Turk Journal of Medical Science.41(6):1043-1049.

      [38] Sacchetti ,G., Maietti,S., Muzzoli,M., Scaglianti,M., Manfredini, S., Radice, M., Bruni, R.(2005) .Comparative Evaluation of 11 Essential Oils of Different Origin as Functional Antioxidants, Antiradicals and Antimicrobials in Foods. Food Chemistry .91:621-632.http://dx.doi.org/10.1016/j.foodchem.2004.06.031.

      [39] Singh,A., Sharma,P.K., Garg,G. (2010).Natural Products as Preservatives. International Journal of Pharma and Bio Sciences. 1(4):601-612.

      [40] Gowda, N. K., Ledoux, D. R., Rottinghaus,G. E., Bermudez, A. J., Chen, Y. C.(2008). Efficacy of Turmeric (Curcuma longa), Containing a known Level of Curcumin, and a Hydrated Sodium Calcium aluminosilicate to Ameliorate the Adverse Effects of Aflatoxin in Broiler Chicks. Poult Science. 87(6):1125-1130.http://dx.doi.org/10.3382/ps.2007-00313.

      [41] Jang, H-D., Chang,K-Sh., Huang, Y-Sh., Hsu,Ch-L., Lee,Sh-H ., Su,M-Sh.(2007).Principal Phenolic Phytochemicals and Antioxidant Activities of Three Chinese Medicinal Plants. Food Chemistry.103: 749-756.http://dx.doi.org/10.1016/j.foodchem.2006.09.026.

      [42] Mongkolsilp, S., Pongbupakit,I., Sae-Lee N ., Sitthithaworn ,W. (2004).Radical Scavenging Activity and Total Phenolic Content of Medicinal Plants used in Primary Health Care.SWU J Pharm Sci.9 (1):32-35.

      [43] Maizura, M., Aminah, A., Wan Aida, W. M. (2011).Total Phenolic Content and Antioxidant Activity of Kesum (Polygonum minus), Ginger (Zingiberofficinale) and Turmeric (Curcuma longa) Extract. International Food Research Journal. 18: 529-534.

      [44] Quiles, J. L., Mesa, M. D., Ramírez-Tortosa, C. L., Aguilera,C. M., Battino,M. Gil, Ã., Ramírez-Tortosa, M. C.(2002).Curcuma longa Extract Supplementation Reduces Oxidative Stress and Attenuates Aortic Fatty Streak Development in Rabbits.ArteriosclerThrombVasc Biol.22:1225-1231.http://dx.doi.org/10.1161/01.ATV.0000020676.11586.F2.

      [45] Tiwari, V., Shanker, R., Srivastava, J., Vankar, P. S.( 2006).Change in Antioxidant Activity of Spices-Turmeric and Ginger on Heat Treatment. Electron. J. Environ. Agric. Food Chem. 5 (2):1313-1317.

      [46] Salem, M. L. (2005). Review:Immunomodulatory and Therapeutic Properties of the Nigella sativa L. Seed. International Immunopharmacology 5:1749-1770.http://dx.doi.org/10.1016/j.intimp.2005.06.008.

      [47] Bourgou, S., Ksouri ,R., Bellila ,A., Skandrani ,I., Falleh ,H., Marzouk, B.(2008).Phenolic Composition and Biological Activities of TunisianNigella sativaL. Shoots and Roots.Biologies.7:331-335.http://dx.doi.org/10.1016/j.crvi.2007.11.001.

      [48] Singh,G., Marimuthu, P., De heluani, C. S., Catalan, C. (2005). Chemical Constituents and Antimicrobial and Antioxidant Potentials of Essential Oil and Acetone Extract of Nigella sativa Seeds.Journal of the Science of Food and Agriculture. 85:2297-2306. http://dx.doi.org/10.1002/jsfa.2255.

      [49] El-Beshbishy, H. A., Mohamadin, A. M., Abdel-Naim, A. B. (2009) .In Vitro Evaluation of the Antioxidant Activities of Grape Seed (Vitisvinifera) Extract, Blackseed (Nigella sativa) Extract and Curcumin. Journal of Taibah University Medical Sciences.4(1):23-35.http://dx.doi.org/10.1016/S1658-3612(09)70078-2.

      [50] Yoruk, O., Gur, F. O., Uyanik, H., Yasar,M., Mutlu,V., Altas,E., Baysal, E., Taysi,S.(2010).Antioxidant Effects of Nigella Sativa in the Treatment of Experimentally Induced Rhinosinusitis. Macedonian Journal of Medical Sciences. 3(2):132-137.http://dx.doi.org/10.3889/MJMS.1857-5773.2010.0101.

      [51] Tülüce, Y., Özko,H., Söģüt, B., Çelik, İ.( 2009) .Effects of Nigella sativa L. on Lipid Peroxidation and Reduced Glutathione Levels in Erythrocytes of Broiler Chickens. Cell Membranes and Free Radical Research. 1(1):95-99.

      [52] Musa, D., Dilsiz, N., Gumushan, H., Ulakoglu, G., Bitlren, M.(2004). Antitumor Activity of an Ethanol Extract of Nigella sativa Seeds. Biologia, Bratislava.59:735-740, 2004.

      [53] Kanter, M., Demir, H., Karakaya, C., Ozbek, H. (2005). Gastroprotective Activity of Nigella sativa L Oil and its Constituent, Thymoquinone against Acute Alcohol-Induced Gastric Mucosal Injury in Rats. World Journal of Gastroenterology .11(42):6662-6666.

      [54] Ramadan, M.F and Mörsel, J.T. (2003). Analysis of glycolipids from black cumin (Nigella sativa L.), coriander (Coriandrum sativumL.) and Niger (Guizotia abyssincaCass.) oilseeds. Journal of Food Chemistry. 80:197-204.http://dx.doi.org/10.1016/S0308-8146(02)00254-6.

      [55] Paterson, P.(2009). Neem the Wonder Tree: Its Pesticide and Medicinal Applications.M.Sc Thesis. Maryland University. Washington.USA.

      [56] Anokwuru, C.P., Anyasor, G.N., Ajibaye O., Fakoya O., Okebugwu P. (2011).Effect of Extraction Solvents on Phenolic, Flavonoid and Antioxidant Activities of Three Nigerian Medicinal Plants. Nature and Science.9(7):53-61.

      [57] Bhowmik, D., Yadav, C. J., Tripathi,K. K., Kumar, K. P. (2010).Herbal Remedies of Azadirachta indicaand its Medicinal Application. Journal of Chemistry and Pharmacy Research. 2(1):62-72.

      [58] Reddy, P.M., Gobinath,M., Rao, K.M., Venugopalaiah, P., Reena,N. (2011).A review on Importance of Herbal Drugs in Cosmetics. International Journal of Advances in Pharmacy and Nanotechnology.1 (3):121 -139.

      [59] Adu, F., Gbedema, S. Y., Brown, P., Annan, K., Boamah, V. E. (2011). Antibacterial and Free Radical Scavenging Activity of Durantaplumieri, Linn. International Journal of Pharmaceutical Sciences and Research (JPSR).2 (2):282-287.

      [60] Narayanaswamy,N and Balakishnan,K.P.(2011).Evaluation of Some Medicinal Plants for their Antioxidants Properties. International Journal of Pharm Tech Research.3(1):381-385.

      [61] Shaheen, F., ahmad, M., khan, M. T., jalil, S., Ejaz, A., Sultankhodjaev M. N., Arfan, M., Choudhary, M. I., Rahman, A.U. (2005). Alkaloids of Aconitum Leave and their Anti-inflammatory, Antioxidant and Tyrosinase Inhibition Activities. Phytochemistry. 66: 935-940.http://dx.doi.org/10.1016/j.phytochem.2005.02.010.

      [62] Zhu, K., Zhou, H., Qian, H. (2006): Antioxidant and Free Radical-Scavenging Activities of Wheat Germ Protein Hydrolysates (WGPH) Prepared with Alcalase. Process Biochemistry. 41:1296-1302.http://dx.doi.org/10.1016/j.procbio.2005.12.029.

      [63] Balois, M.S.(1958).Antioxidants Determination by the use of Stable Free Radical.Nature.4617:1199-1200.http://dx.doi.org/10.1038/1811199a0.

      [64] Yokozawa,T.,Chen,C.P.,Dong,E.,Tanka,T.,Nonaka,G.I.,Nishioka,I.(1998).Study on the Inhibitory Effect Tannins and Flavonoids against the 1,1-Diphenyl-2-Picryl Hydazyl Radical.Biochem.Pharmacol.56:213-222.http://dx.doi.org/10.1016/S0006-2952(98)00128-2.

      [65] Kim, H. P.,S on, K. H., Chang, H. W., Kang, S. S. (2004). Antiinflammatory Plant Flavonoids and Cellular Action Mechanisms. Journal of pharmacology .96:229-245.

      [66] Parfitt, K. (1999). “Martindale ", Disinfectant and Preservatives. The Pharmaceutical Press,32 ed.1097.

      [67] Barros, L., Ferreira, M.J., Guciros, B., Ferreira, I.C., Baptista, P. (2007).Total Phenols, Ascorbic Acid, β-carotene and Lycopene in Portuguese Wild Edible Mushroom and their Antioxidant Activities. Food Chemistry.103:413-420. http://dx.doi.org/10.1016/j.foodchem.2006.07.038.

      [68] Singh, R., Singh, H.,Kumar, S., Arorra, S. (2007). Studies on Antioxidant Potentials of Methanol Extract/ Fraction of Acacia auriculi.ACunn.Food Chemistry.103:505. http://dx.doi.org/10.1016/j.foodchem.2006.08.019.

      [69] Bondet, V.,Brand,-Williams, W., Berset,C.(1997).Kinetics and Mechanisms of Antioxidant activity Using the DPPH Free Radical Method. Lebenon-Wiss U-Technol.30:609-615.http://dx.doi.org/10.1006/fstl.1997.0240.

      [70] Ona, M., Oda, E., Tanka, T., Idia, Y., Yamasakki, T., Masuoka, C.(2008). DPPH Radical ScavengingEffect on Some Constituents from the Arial Part of Lippin triphya. J. Nat Med.62 (1):101-106.http://dx.doi.org/10.1007/s11418-007-0197-9.

      [71] Finkel, T and Holbrook, N.T. (2001).Oxidants, Oxidative Stress Biology of Ageing[77] Ferrali, M., Donati, D., Bambagioni, S., Fontani, M.,Giorgic, G., Pietrangelod, A.(2001).3-Hydroxy-(4H)-benzopyran-4-ones as Potential Iron Chelating Agents In Vivo. Bioorganic and Medicinal Chemistry. 9:3041-3047. http://dx.doi.org/10.1016/S0968-0896(01)00207-3.

      [72] Navana, M.C., Mantilla, M.P., Martin, A. (1992). Free Radical and Antihepatotoxic Activity of Rosmarius tomentosus.Planta Med.59:312-14.

      [73] Vijayanand, S., and Hemapriya, J.(2011).In vitro Antibacterial Efficacy of Peel and Seed Extracts of Punica granatumL.against Selected Bacterial Strains. International Journal of Medicobiological Research. 1(4):231-234.

      [74] Ramadan, M.F., Kroh,L. W., Mörsel, J.T. ( 2003). Radical Scavenging Activity of Black Cumin (Nigella sativa L.), Coriander (Coriandrum sativumL.), and Niger (Guizotia abyssinicaCass.) Crude Seed Oils and Oil Fractions. Journal of Food Chemistry.1-9.

  • Downloads

  • How to Cite

    EL-Kamali, H., & E. M. Hamed, S. (2015). Antioxidant potential of some Sudanese medicinal plants used in traditional medicine. International Journal of Scientific World, 3(2), 192-198. https://doi.org/10.14419/ijsw.v3i2.4741