Isolation and Characterization of Bromelain (BML) Proteases from Ananas cosmosus an asset to Cancer Chemotherapy
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Abstract
Chemo impediment impels the quest for moreover single targeted or brew of multi-targeted agents. Bromelain, potential agent in this regard, is a pharmacologically active compound, present in stems and fruits of Ananas cosmosus, endowed with anti-inflammatory, anti-invasive and anti-metastatic properties. Bromelain is a complex or proteolytic enzymes. These proteolytic enzymes are paraphernalia that promise an impressive number of medical and therapeutic uses, particularly as anti-tumour agents. The purpose of this study was to develop a lead range of BML extraction process. The purification of the enzyme was carried out by precipitation and by chromatography techniques. Further, the drying of chromatographically purified fraction was carried out in freeze dryer and spray dryer. The activity was expressed in terms of casein digesting units (CDU). The BML extract of higher purity was obtained from the fruit and stem using ion exchange chromatography. The elution competence in chromatographic purification was obtained in the range of 90%. Bromelain thus obtained was subjected to spray and freeze drying. The BML was extracted using stem yielded a total protein content of 242.6 mg/mL with the proteolytic activity of 878.14 GDU/mL. The extracted Bromelain was characterized using the standard spectroscopic techniques such as IR Spectroscopy, UV-Spectroscopy, LCMS and PXRD to understand the structure of the lead molecule acting on the cancer cells.
References
M.B. Doko, V. Bassani, J. Casadebaig, L. Cavailles, M. Jacob,Preparation of proteolytic enzyme extracts from Ananas comosus L.Merr. fruit using semi permeable membrane, ammonium sulphate extraction, centrifugation and freeze drying processes, International Journal of Pharmaceutics Vol. 76 (1991) pp.199-206.
J.C. Caygill, Sulphydryl Plant Proteases, Journal of Enzyme and Microbial Technology Vol.1, Issue 4, (1979) pp. 233-242.
S. Ota, S. Moore, W.H. Stein, Preparation and Chemical properties of purified Stem and Fruit Bromelains Journal of Biochemistry Vol.3 (1964) pp.180-185.
T. Murachi, M. Yasui, Y. Yasuda, Purification and Physical Characterization of Stem Bromelain, Journal of Biochemistry Vol.3 (1964) pp.48-55.
R.K. Scopes, in: R.K. Scopes (Ed.), Protein Purification: Principles and Practice, second edition Springer-Verlag, New York, 1982, pp. 41-65.
View more references (10)
R. Arnon, E. Shapira, Antibodies to papain. A selective fractionation according to inhibitory capacity, Journal of Biochemistry Vol. 6 (1967) pp.3942-3950.
G.R. Dapeau, Methods in Enzymology Vol.45, p.471. Academic press, Newyork, 1976
P.K. Smith, P.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, O.C. Klenk, Measurement of protein using bicinchoninic acid Journal of Analytical Biochemistry, Vol.150 (1985) pp.76-85.
Y. Liao, M.B. Brown, G.P. Martin, Investigation of the stabilisation of freeze-dried lysozyme and the physical properties of the Formulations European Journal of Pharmacy and Biopharmaceuticals Vol.58 (2004) pp.15-24.
K. Samborska, D. Witrowa-Rajchert, A. Gonclaves, Spray drying of alpha-amylase-the effect of process variables on the enzyme inactivation, Drying Technology, Vol.23, Issue.4, (2005) pp. 941-953.
P.S. Kuts, E.G. Tutova, Fundamentals of Drying of Microbiological Materials, Drying Technology. Vol.2 Issue 2, (1983-84) pp.171-201.
T. Tsuda, M. Uchiyama, T. Sato, H. Yoshino, Y. Tsuchiya, S. Ishikawa, M. Ohmae, S.Watanabe, Y. Miyake, Mechanism and Kinetics of secretion degradation in aqueous solutions, Journal of Pharm. Sci. Vol.79 (1990) pp. 223-227.
H. R. Maurer Bromelain: biochemistry, pharmacology and medical use, CMLS, Journal of Cellular Molecular Life Sciences Vol.58 (2001) pp. 1234-1245.
R.V Devakatte, V.V Patil, S.S. Waje, B.N.Throat, Purification and drying of Bromelain, Journal of Separation and Purification Technology Vol. 64, (2009) pp.259-264.
Melissa Loh Pei Shian, Nur Aisyah Samsul Baharil, Ting Lee Yu, M. Taher and Fadzilah Adibah Abdul Majid, Pilot scale extraction of proteolytic enzyme bromelain from Ananas comosus, Faculty of Chemical and Natural Resources Engineering, University of Technology Malaysia, 2005.