Antioxidant efficacy of synthesized composite copper lutein nano nutraceuticals ‎from brassica oleracea var italica

  • Authors

    • Saif Saadee Nawaf Department of Biotechnology, Mangalore University, Mangalagangothri, Mangalore
    • Sudhakar Malla Department of Microbiology, Mangalore University, Jnana Kaveri, Chikka Aluvara, Kodagu
    • Manjula Ishwara Kalyani Insearch Biotech laboratory, Bangalore
    https://doi.org/10.14419/fwg9s764

    Received date: April 15, 2025

    Accepted date: May 21, 2025

    Published date: May 26, 2025

  • Copper Nanoparticles; Broccoli; FTIR; Antioxiants; Lutein; DPPH; Ferrozine Assay; RP ‎Method

  • Abstract

    Aim: Due to its antioxidant properties, lutein scavenges reactive oxygen species, including lipid ‎peroxy radicals and singlet oxygen. Despite the therapeutic promise derived from its various ‎physiological functions, lutein's absorption and stability issues make efficient distribution ‎challenging. Nanotechnology applications in pharmaceuticals have been the subject in recent decades. Similar to nanopharmaceuticals, a new class of nanomaterials known ‎as nanonutraceuticals has evolved due to the application of nanotechnology for improved ‎nutraceutical delivery.‎

    Methods: For green synthesis, B. oleracea stalks and florets were utilized individually in a ‎copper sulphate solution. The synthesized Copper nanoparticles were characterized and screened ‎for their antibacterial and anti-biofilm activities. ‎

    Results: At 400μg/ml concentrations, FLNP and FNP demonstrated 92 and 88% DPPH free ‎radical scavenging, respectively. Comparing the different NPs to the positive control, FLNP ‎demonstrated the highest H2O2 scavenging activity (IC50 72.55μg/ml), followed by FNP. ‎When compared to the positive control, FLNP, FNP, and Lutein demonstrated antioxidant ‎capacities of 94, 91, and 87% at 400µg/ml using the reducing power method. According to our ‎ferrozine assay results, FLNP demonstrated an efficient ability to bind iron, suggesting that this ‎property may be linked to its antioxidant properties. According to their different antioxidant ‎capacities, FLNP and FNP demonstrated 92 and 88% FZ reduction ability. ‎

    Conclusion: The present study was successful in synthesizing composite lutein-based copper ‎nanoparticles and also confirmed of the potential antioxidant activity exhibited by the ‎composite particles. The composite particles did exhibit enhanced antioxidant activity when ‎compared to other controls‎.

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    Nawaf, S. S. ., Malla, S. ., & Kalyani , M. I. . (2025). Antioxidant efficacy of synthesized composite copper lutein nano nutraceuticals ‎from brassica oleracea var italica. International Journal of Basic and Applied Sciences, 14(1), 445-452. https://doi.org/10.14419/fwg9s764