In vitro callus induction of gerbera from leaf explant

Authors and Affiliations

  • Sadia Afrin Jui Bangladesh Jute Research Institute, Dhaka, Bangladesh
  • Md. Mijanur Rahman Rajib Bangabandhu Sheikh Mujibur Rahman Agricultural University
  • M. Mofazzal Hossain Bangabandhu Sheikh Mujibur Rahman Agricultural University
  • Sharmila Rani Mallik Bangladesh Agricultural Research Institute image/svg+xml
  • Iffat Jahan Nur Bangladesh Jute Research Institute image/svg+xml
  • Jannatul Ferdush Bangladesh Jute Research Institute image/svg+xml

About this article

DOI:

https://doi.org/10.14419/ijag.v8i1.30000

Received:

20-10-2019

Accepted:

30-11-2019

Published:

11-01-2020

Views:

622

Downloads:

270
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Keywords:

Callus, Gerbera, Growth Regulators, Leaf Explants, Culture.

Abstract

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.

 

 

 


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How to Cite

Afrin Jui, S., Mijanur Rahman Rajib, M., Mofazzal Hossain, M., Rani Mallik, S., Jahan Nur, I., & Ferdush, J. (2020). In vitro callus induction of gerbera from leaf explant. International Journal of Advanced Geosciences, 8(1), 1-4. https://doi.org/10.14419/ijag.v8i1.30000

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