Influence of solvents on polyphenol content and antioxidant activity of fig leaf extracts obtained by maceration and ultrasonic extraction
Keywords:Fig Leaf, Polyphenols, FRAP, DPPH Inhibition.
In this paper, the polyphenol content and antioxidant activity of fig leaf extracts were analyzed. Extraction was performed with methanol, ethanol, acetone and their aqueous mixtures (50:50 v/v). Extractions were performed with stirring at 300 rpm on a vibromix and ultrasonic bath for 15 minutes. The polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu test. Antioxidant capacity was tested using the FRAP and DPPH methods. The results showed a significant effect of extraction of bioactive components using an organic solvent:water mixture in relation to the organic solvent itself. Ultrasonic extraction proved to be a more efficient technique compared to mixing at 300 rpm.
 Abdel-Aziz MD, Darwish MS, Mohamed AH, El-Khateeb AY & Hamed SE (2020), Potential Activity of Aqueous Fig Leaves Extract, Olive Leaves Extract and Their Mixture as Natural Preservatives to Extend the Shelf Life of Pasteurized Buffalo Milk. Foods 9(5), 615. https://doi.org/10.3390/foods9050615.
 Altiok E, BayÃ§in D, Bayraktar O & ÃœlkÃ¼ S (2008), Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin. Separation and Purification Technology 62, 342-348. https://doi.org/10.1016/j.seppur.2008.01.022.
 Benzie IFF, Strain JJ (1999), Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299, 15-27. https://doi.org/10.1016/S0076-6879(99)99005-5.
 Bimakr M., Rahman RA, Taip FS, Ganjloo A, Salleh LM, Selamat J, Hamid A & Zaidul ISM (2011), Comparision of different extraction methods for the extraction of major flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and Bioproducts Processing 89, 67-72. https://doi.org/10.1016/j.fbp.2010.03.002.
 Garza-Alonso CA, Olivares-SÃ¡enz E, GutiÃ©rrez-DÃez A, VÃ¡zquez-Alvarado RE & LÃ³pez-JimÃ©nez A (2019), Visual Symptoms, Vegetative Growth, and Mineral Concentration in Fig Tree (Ficus carica L.) Under Macronutrient Deficiencies. Agronomy 9(12), 787. https://doi.org/10.3390/agronomy9120787.
 HoroziÄ‡ E, ZukiÄ‡ A, KolareviÄ‡ L, BjeloÅ¡eviÄ‡ D, AdemoviÄ‡ Z, Å ariÄ‡-KundaliÄ‡ B, HusejnagiÄ‡ D, KudumoviÄ‡ A, HamziÄ‡ S (2019) Evaluation of an-tibacterial and antioxidant activity of methanol needle extracts of Larix Decidua Mill., Picea Abies (L.) H. Karst. and Pinus Nigra J. F. Arnold. Technics Technologies Education Management 14(1), 14-19.
 Kannur D & Khandelwal K (2014), Pharmacognostical investigation and evaluation of Ficus carica fruit extract for adaptogenic activity. International Journal of Pharmaceutical Sciences and Research 5(5), 2022-2032. https://doi.org/10.13040/IJPSR.0975-8232.5(5).2022-32.
 Kucukerdonmez O, Baygeldi N, Akder RN & Ã‡aÄŸÄ±ndÄ± Ã– (2021), Medicinal and Nutritional Analysis of Fig (Ficus carica) Seed Oil; A New Gamma Tocopherol and Omega-3 Source. Progress in Nutrition 23(2), 1-6.
 Lazreg Aref H, Gaaliche B, Fekih A, Mars M, Aouni M, Pierre Chaumon J, Said K (2011), In vitro cytotoxic and antiviral activities of Ficus carica latex extracts. Natural Product Research, 25(3), 310-319. https://doi.org/10.1080/14786419.2010.528758.
 Mawa S, Husain K & Jantan I (2013), Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and Biological Activities. Evidence-Based Complementary and Alternative Medicine 2013. https://doi.org/10.1155/2013/974256.
 Mopuri R, Ganjayi M, Meriga B, Koorbanally NA & Islam S (2018), The effects of Ficus carica on the activity of enzymes related to metabolic syndrome. Journal of Food and Drug Analysis 26(1), 201-210. https://doi.org/10.1016/j.jfda.2017.03.001.
 Oliveira AP, ValentÃ£o P, Pereira JA, Silva BM, Tavares F, Andrade PB (2009), Ficus carica L.: Metabolic and biological screening. Food and Chemical Toxicology 47(11), 2841-2846. https://doi.org/10.1016/j.fct.2009.09.004.
 Rubnov S, Kashman R, Rabinowiz M, Schlesinger M, Mechoulam R (2001) Suppressors of cancer cell proliferation from fig (Ficus carica) resin: isolation and structure elucidation. Journal of Natural Products 64(7), 993-996. https://doi.org/10.1021/np000592z.
 Singleton VL, Orthofer R, Lamuela-RaventÃ³s RM (1999), Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1.