Comparative Study of Bactericidal Activity of Blood Serum against Escherichia Coli in Urban and Slum Population of Bangladesh


  • Mehnaz Karim Fahareen Binta Mosharraf
  • Shabnam Ahsan
  • . .



Blood serum, Urban, Rural, Regression analysis.


Bangladesh is a high densely populated country. Due to the lack of socio economic development it is exposed to lots of water-borne diseases. Of them, Escherichia coli or E.coli is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of humans and can cause Bloody Diarrhea. This study will help us to analyze whether the exposure of E.coli, may accompaniment the activity of human blood serum, which is affected by the socio economic difference in the privileged and under privileged population of Bangladesh. The lifestyle of these two groups are quite different. They do not have similar facilities (in terms of balanced nutritional availability and standard vaccination plan) and hygiene conditions. The population living in slum areas, often privations these and hence expected to be more exposed to the pathogen E.coli. The design of this experiment is based on regression analysis by varying serum activity against E.coli at 0 minute (initial time) and at 180 minutes, observed in benefited and slum population of urban areas, which has been checked for adequacy and significance via Excel. It will improve our thought about the exposure of various population groups to this bacteria and their response against it. However the under privileged group showed more effective complement mediated killing in comparison to privileged population.




[1] Black RE, Brown KH, Becker S, Alim ARMA, Merson MH (1981). Contamination of weaning foods and transmission of enterotoxigenic Escherichia coli diarrhoea in children in rural Bangladesh. Transactions Royal Soc Tropical Med & Hyg 76: 259-264.

[2] Bentley R, Meganathan R (1982). Biosynthesis of vitamin K (menaquinone) in bacteria. Microbiol Rev 46: 241–80.


[4] Albert MJ, Faruque SM, Faruque AS, Neogi PK, Ansaruzzaman M, Bhuiyan NA, Alam K, Akbar MS (1995). Controlled study of Escherichia coli diarrheal infections in Bangladeshi children. J Clin Microbiol 33: 973–977.

[5] Griffin PM (1995). Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli. In: Blaser MJ, Smith PD, Ravdin JI, Greenberg HB, Guerrant RL, editors. Infections of the gastrointestinal tract. New York, USA: Raven Press, pp. 739–761.

[6] Cassels FJ, Wolf MK (1995). Colonization factors of diarrheagenic E. coli and their intestinal receptors. J Ind Microbiol 15: 214–226.

[7] Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV (1999). Food-related Illness and Death in the United States. Emerg Infect Dis 5: 607-625.

[8] Cowden JM (1997). Scottish outbreak of Escherichia coli O157, November-December 1996. Euro Surveill, 2: 134. Retrieved December 28, 2015, from:

[9] Blattner FR, Plunkett III G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF et al. (1997). The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1474.

[10] William S. Cleveland, Susan J. Devlin, Locally weighted Regression: an approach to regression analysis by local fitting, American Statistical Association, Vol. 83, 1998, Issue 403;

[11] Bowers J, et al. (1999) A mutation in the MSH6 subunit of the Saccharomyces cerevisiae MSH2-MSH6 complex disrupts mismatch recognition. J Biol Chem 274(23):16115-25

[12] Eisenstein B, Zaleznik D (2000). Enterobacteriiaceae. In: Mandell GL, Douglas, Bennett J, editors. Principles and Practice of Infectious Diseases. 5th ed. United States: Churchill Livingstone, pp. 2294-2310.

[13] De Rycke J, Oswald E (2001). Cytolethal distending toxin (CDT): A Bacterial Weapon to Control Host Cell Proliferation. FEMS Microbiol Lett 203: 141–148.

[14] Feng P, Weagant SD, Grant MA, Burkhardt W (2002). Enumeration of Escherichia coli and the Coliform Bacteria. 8th ed. USA: BAM.

[15] Brenner DJ, Krieg NR, Staley JT (2005). The Proteobacteria, The Gammaproteobacteria. In: Garrity, Brenner G, Don J, Krieg, Noel R, Staley, James R, editors. Bergey's Manual of Systematic Bacteriology 2B. 2nd ed. New York: Springer, pp. 1108.

[16] Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA (2005). Diversity of the human intestinal microbial flora. Science 308: 1635–1638.

[17] Ahmed A, Li J, Shiloach Y, Robbins J, Szu S (2006). Safety and Immunogenicity of Escherichia coli O157 O-specific Polysaccharide Conjugate Vaccine in 2-5-year-old children. J Infect Dis 193: 515–521.

[18] Centers for Disease Control and Prevention. (2006). Update on Multi-State Outbreak of E. coli O157:H7 Infections from Fresh Spinach. Retrieved December 27, 2015 from

[19] Food and Drug Administration. (2006). FDA news: FDA warning on serious foodborne E. coli O157:H7 outbreak. Rockville (MD): US Department of Health and Human Services. Retrieved December 25, 2015, from

[20] Abbadi SH, Strockbine NA (2007). Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Egypt J Med Microbiol 16: 225.

[21] Fratamico P (2007). Non-O157 Shiga Toxin-Producing E. coli Associated with Muscle Foods. Meeting abstract. Retrieved December 25, 2015, from

[22] Thompson A (2007). E. coli Thrives in Beach Sands. Live Science. Retrieved December 14, 2015, from

[23] Giovanni C. Porzio, Regression Analysis by Example. Volume 49, 2007 - Issue 2. Published online: 1 Jan 2012. Journal of Applied Statistics.

[24] Aarestrup FM, Wegener HC, Collignon P (2008). Resistance in bacteria of the food chain: epidemiology and control strategies. Expert Rev Anti Infect Ther 6: 733–750.

[25] Bugla-Ploskońska G, Kiersnowski A, Futoma-Kotoch B, Doroszkiewicz W (2009). Killing of Gram-Negative Bacteria with Normal Human Serum and Normal Bovine Serum: Use of Lysozyme and Complement Proteins in the Death of Salmonella strains O48. Microb Ecol 58: 276-289.

[26] Gould LH, Bopp C, Strockbine N, Atkinson R, Baselski V, Body B, Carey R, Crandall C, Hurd S, Kaplan R et al. (2009). Recommendations for Diagnosis of Shiga Toxin–Producing Escherichia coli Infections by Clinical Laboratories. Morbidity and Mortality Weekly Review 88: 1-14. Retrieved December 13, 2015 from

[27] Black RE, Cousens S, Johnson HL, Lawn JE, Rudan I, Bassani DG, Jha P, Campbell H, Walker CF, Cibulskis R et al. (2010). Global, Regional and National Causes of Child Mortality in 2008: A Systematic Analysis. Lancet 375: 1969-1987.

[28] CNN (2010). German-grown food named likely culprit in deadly outbreak. Retrieved December 26, 2015, from

[29] Bloch EF, McDonald-Pinkett S, Campbell S, Baskin S, Dillahunt S, Peters S, Lucas S, Evans D, Johnson C, Everett T, Kanaan Y (2011). New mechanism for complement killing of Gram-negative bacteria. Afr J Microbiol Res 5: 3936-3941.

[30] Denn R (2011). Poisoned author Jeff Benedict examines the current state of food safety in the US. The Christian Science Monitor. Boston, MA.

[31] Federal Institute for Risk Assessment (2011). High probability of responsibility of fenugreek seeds for EHEC O104: H4 out-break. Retrieved December 25, 2015, from

[32] European Food Safety Authority (2012). E. coli: Rapid response in a crisis. Retrieved December 26, 2015, from

[33] Boland KG, Hayles AN, Miller CB, Kerr T, Brown WC, Lahmers KK (2013). Regional Immune Response to Immunization with Escherichia coli O157:H7-Derived Intimin in Cattle. Clin Vaccine Immunol 20: 562-571.

[34] Croxen MA, Finlay BB (2013). Molecular mechanisms of Escherichia coli pathogenicity. Nat Rev Microbiol 11: 141.

[35] Gülden Kaya Uyanık,Neşe Güler, A study on multiple Linear Regression Analysis, Procedia - Social and Behavioral Sciences, Volume 106, 10 December 2013,

[36] Bennington-Castro J (2014). Treatment for an E. coli Infection. Everyday Health. Retrieved December 28, 2015, from

[37] Everyday Health (2014). Symptoms of an E. coli Infection. Retrieved December 25, 2015, from

[38] Shuling Wang, Lin Zheng, Jiangtao Dai, Empirical Likelihood Diagnosis of Modal Linear Regression Models, Journal of Applied Mathematics and Physics Vol.2 No.10, 2014,

[39] Madappa, T. (2015, October). Escherichia coli Infections Clinical Presentation. Medscape. Retrieved December 24, 2015.

[40] CDC (2014). E. coli Infection and Food Safety. Retrieved December 25, 2015, from

[41] CDC (2014). Bacterial Meningitis. Retrieved December 25, 2015, from

[42] Ashida H, Mimuro H, Sasakawa C (2015). Shigella Manipulates Host Immune Responses by Delivering Effector Proteins with Specific Roles. Front Immunol 6: 219.

[43] Centers for Disease Control and Prevention. (2006). CDC health alert: multiple states investigating a large outbreak of E. coli O157:H7 infections. Atlanta: US Department of Health and Human Services. Retrieved December 27, 2015 from

[44] CDC (2015). Multistate Outbreaks of Shiga toxin-producing Escherichia coli O26 Infections Linked to Chipotle Mexican Grill Restaurants. Retrieved December 27, 2015, from

View Full Article:

How to Cite

Karim Fahareen Binta Mosharraf, M., Ahsan, S., & ., . (2018). Comparative Study of Bactericidal Activity of Blood Serum against Escherichia Coli in Urban and Slum Population of Bangladesh. International Journal of Engineering & Technology, 7(2.32), 513–518.
Received 2019-03-30
Accepted 2019-03-30