Histological Analysis of Murine Ovaries and Uteruses Following Supplementation with Alpha-Tocopherol in Nicotine Injected Mice

  • Authors

    • Mohd Azmil Amirudin
    • Muhammad Shahrizal Sanudin
    • Mohd Shahrul Nizam Mohd Isa
    • Siti Syairah Mohd Mutalip
    2018-12-03
    https://doi.org/10.14419/ijet.v7i4.38.27910
  • Vitamin E, Alpha-Tocotrienol, Tocotrienols, Ovary, Uterus

  • Vitamin E can be classified into eight different lipophilic subtypes; namely alpha(α)-, beta(β)-, delta(δ)- and gamma(γ)-tocopherol (TOC) as well as alpha(α)-, beta(β)-, delta(δ)- and gamma(γ)-tocotrienol (TCT). From these isoforms, α-TOC has been reported to have the most promising health benefits. The purpose of this study was to determine the effect of α-TOC on the histological structures of murine ovaries and uteruses in nicotine-treated females. Thirty ICR female mice were divided into 5 groups (Group A – E) with 6 mice each. Group A received 0.1 ml of tocopherol-stripped corn oil, group B given 0.1 ml of 0.9% normal saline, group C treated with 3 mg/kg/day of nicotine, group D treated with 60 mg/kg/day of alpha-tocopherol and group E was concurrently treated with 3 mg/kg/day of nicotine and 60 mg/kg/day of alpha-tocopherol. Following the treatments, all females were euthanized and the studied organs were collected and processed for histological analysis. Obtained results indicated that exposure to nicotine reduced the numbers of ovarian follicles and endometrium thickness (Group C). However, these adverse effects have been reduced in the group concurrently treated with nicotine and α-TOC (Group E). This result suggested that α-TOC might be able to minimize the adverse effects of nicotine in the ovaries and the uterus. Further studies are needed to confirm on these effects from the physiological aspects of the ovaries and uteruses.

     

  • References

    1. [1] Alawin, O. A., Ahmed, R.A., Ibrahim, B.

      [2] A., Briski, K.P., & Sylvester, P.W. 2016. Antiproliferative effects of γ-tocotrienol are associated with lipid raft disruption in HER2-positive human breast cancer cells. The Journal of Nutritional Bichemistry, 27:266-277.

      [3] Azzi, A. 2007. Molecular mechanism of

      [4] alpha-tocopherol action. Free Radical Biology & Medicine, 43: 16-21.

      [5] Dechanet, C., Anahory, T., Mathieu Daude, J.C., Quantin, X., Reyftmann, L., Hamamah, S., Hedon, B., & Dechaud, H. 2011. Effects of cigarette smoking on reproduction. Human Reproduction Update, 17(1): 76–95.

      [6] Dumanına, S., Kalan, M., Tokoferalinin, S., & Stres, O. 2014. The Effect of α -Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes.

      [7] Evans, H. M., Bishop, K. S. 1922. On the existence of a hitherto unrecognized dietary factor essential for reproduction. Science, 56: 650-651.

      [8] Huang, J., Okuka, M., McLean, M., Keefe, D.L., & Liu, L. 2009. Effects of cigarette smoke on fertilization and embryo development in vivo. Fertility and Sterility, 92(4), 1456-65. doi: 10.1016/j.fertnstert.2008.07.1781

      [9] Hughes, E.G., Lamont, D.A., Beecroft, M.L., Wilson, D.M., Brennan, B.G., Rice, S.C. 2000. Randomized trial of a ‘stage-of-change’ oriented smoking cessation intervention in infertile and pregnant women. Fertility & Sterility, 74:498–503.

      [10] IUPAC-IUB Joint Commission on Biochemical Nomenclature. 1982. Nomenclature of tocopherols and related compounds. (Recommendations 1981). European Journal of Biochemistry, 123: 473-475.

      [11] Kamsani, Y.S., Rajikin, M.H., Nor-Ashikin, M.N.K., Nuraliza, A.S., & Chatterjee, A. 2013. Nicotine-induced cessation of embryonic development is reversed by γ-tocotrienol in mice. Medical Science Monitor Basic Research, 19:87-92.

      [12] Nasibah, A., Rajikin, M.H., Nor-Ashikin, M.N.K., & Nuraliza, A.S. 2012a. Tocotrienol improves the quality of impaired mouse embryos induced by corticosterone. Proceeding for Symposium on Humanities, Science and Engineering Research (SHUSER2012), 135-138.

      [13] Nasibah, A., Rajikin, M.H., Nor-Ashikin, M.N.K., & Nuraliza, A.S. 2012b. Effects of tocotrienol supplementation on pregnancy outcome in mice subjected to maternal corticosterone administration. Journal of Oil Palm Research, 24: 1550-1558.

      [14] Patil, S. R., Ravindra, Patil, S. R., Londonkar, R., & Patil, S. B. 1998. Nicotine Induced Ovarian and Uterine Changes in Albino Mice, Indian Journal of Physiology and Pharmacology 42(4): 503–508.

      [15] Saleh, H., Omar, E., Froemming, G., Said, R. 2014. Tocotrienol rich fraction supplementation confers protection on the ovary from cyclophasphamide induced apoptosis. Asian Pacific Journal of Tropical Disease, 4(3): 234, doi: 10.1016/S2222-1808(14)60532-6

      [16] Syairah, S.M.M., Rajikin, M.H., Sharaniza, A.R., Nor-Ashikin, N.K. & Kamsani, Y.S. 2016. Chromosomal Status in Murine Preimplantation 2-Cell Embryos Following Annatto (Bixa orellana)-Derived Pure Delta-Tocotrienol Supplementation in Normal and Nicotine-treated Mice. World Applied Sciences Journal. 34(12): 1855-1859.

      [17] USDA Food Composition Databases, 2015. Website. Retrieved 19 November 2017, from http:ndb.nal.usda.gov/

      [18] Weng-Yew, W., Selvaduray, K. R., Ming, C. H., & Nesaretnam, K. 2009. Suppression of tumor growth by palm tocotrienols via the attenuation of angiogenesis. Nutrition and Cancer, 61(3): 367–373.

      [19] Wu, S.J., Liu, P.L., & Ng, L.T. 2008. Tocotrienol-rich fraction of palm oil exhibits anti-inflammatory property by suppressing the expression of inflammatory mediators in human monocytic cells. Molecular Nutrition and Food Research, 52(8): 921-929.

      [20] Zhao, Z., & Reece, E.A. 2005. Nicotine-induced embryonic malformations mediated by apoptosis from increasing intracellular calcium and oxidative stress. Birth Defects Research. Part B, Developmental and Reproductive Toxicology, 74(5): 383–391.

  • Downloads

  • How to Cite

    Azmil Amirudin, M., Shahrizal Sanudin, M., Shahrul Nizam Mohd Isa, M., & Syairah Mohd Mutalip, S. (2018). Histological Analysis of Murine Ovaries and Uteruses Following Supplementation with Alpha-Tocopherol in Nicotine Injected Mice. International Journal of Engineering & Technology, 7(4.38), 1496-1498. https://doi.org/10.14419/ijet.v7i4.38.27910