Milkfish (Chanos Chanos) Gelatin as Biosensor Material for Chromium (III) Detection


  • T P.H. Hutapea
  • Rukisah .
  • Mulyadi .
  • K A. Madurani
  • Suprapto .
  • F. Kurniawan





Biosensor, chanos chanos, chromium (III), cyclic voltammetry, gelatin, tarakan.


Milkfish (Chanos chanos) gelatin was successfully developed as biosensor material. The milkfish bone was obtained from local restaurants in Tarakan, North Borneo, Indonesia. Gelatin was extracted from milkfish bone using acid method at 55°C. Characterization by FTIR showed that milkfish gelatin had similar functional group with commercial gelatin. The gelatin was used as biosensor material for detecting chromium. The gelatin was mixed with carbon in 1:1 ratio to form gelatin/carbon paste modified silver electrode. Electrochemical impedance spectroscopy (EIS) analysis of the gelatin/carbon paste modified silver electrode showed a better conductivity than paraffin/carbon paste modified silver electrode. Performance of the gelatin/carbon paste modified silver electrode in chromium (III) solution was conducted using cyclic voltammetry technique. Measurement was carried out at -1 V to +1 V with scan rate of 100 mV/s in acid and base condition. The best result was shown by gelatin/carbon paste modified silver electrode. It can detect chromium (III) ions at reduction potential of -0.78 V in alkaline condition. Unspecific responses were observed from silver electrode, paraffin/silver electrode, carbon/silver electrode, gelatin/silver electrode and paraffin/carbon paste modified silver electrode. This result can be concluded that the milkfish gelatin obtained have a potential to be developed as chromium (III) biosensor.




[1] F. Ejeian et al., “Biosensors for wastewater monitoring: A review,†Biosensors and Bioelectronics, vol. 118, pp. 66–79, Oct. 2018.

[2] Z. Zulkarnain, S. Suprapto, T. Ersam, and F. Kurniawan, “A Novel Selective and Sensitive Electrochemical Sensor for Insulin Detection,†Indonesian Journal of Electrical Engineering and Computer Science, vol. 3, no. 3, pp. 496–502, 2016.

[3] F. Kurniawan, V. Tsakova, and V. M. Mirsky, “Analytical Applications of Electrodes Modified by Gold Nanoparticles: Dopamine Detection,†Journal of Nanoscience and Nanotechnology, vol. 9, no. 4, pp. 2407–2412, Apr. 2009.

[4] M. Lv, Y. Liu, J. Geng, X. Kou, Z. Xin, and D. Yang, “Engineering nanomaterials-based biosensors for food safety detection,†Biosensors and Bioelectronics, vol. 106, pp. 122–128, May 2018.

[5] J. G. Pacheco, M. F. Barroso, H. P. A. Nouws, S. Morais, and C. Delerue-Matos, “21 - Biosensors,†in Current Developments in Biotechnology and Bioengineering, C. Larroche, M. Ã. Sanromán, G. Du, and A. Pandey, Eds. Elsevier, 2017, pp. 627–648.

[6] M. Chakraborty and M. S. J. Hashmi, “An Overview of Biosensors and Devices,†in Reference Module in Materials Science and Materials Engineering, Elsevier, 2017.

[7] P. Bollella and L. Gorton, “Enzyme based amperometric biosensors,†Current Opinion in Electrochemistry, Jul. 2018.

[8] A. Salim and S. Lim, “Recent advances in the metamaterial-inspired biosensors,†Biosensors and Bioelectronics, vol. 117, pp. 398–402, Oct. 2018.

[9] F. Hodzhaoglu, F. Kurniawan, V. Mirsky, and C. Nanev, “Gold nanoparticles induce protein crystallization,†Crystal Research and Technology, vol. 43, no. 6, pp. 588–593, Jun. 2008.

[10] A. Biscotti, R. Lazzarini, G. Virgilli, F. Ngatcha, A. Valisi, and M. Rossi, “Optimizing a portable biosensor system for bacterial detection in milk based mix for ice cream,†Sensing and Bio-Sensing Research, vol. 18, pp. 1–6, Apr. 2018.

[11] F. Kurniawan, N. S. Al Kiswiyah, K. A. Madurani, and M. Tominaga, “Single-Walled Carbon Nanotubes-Modified Gold Electrode for Dopamine Detection,†ECS Journal of Solid State Science and Technology, vol. 6, no. 6, pp. M3109–M3112, 2017.

[12] A. Soni, R. K. Surana, and S. K. Jha, “Smartphone based optical biosensor for the detection of urea in saliva,†Sensors and Actuators B: Chemical, vol. 269, pp. 346–353, Sep. 2018.

[13] G. Cabral-Miranda, A. R. Cardoso, L. C. S. Ferreira, M. G. F. Sales, and M. F. Bachmann, “Biosensor-based selective detection of Zika virus specific antibodies in infected individuals,†Biosensors and Bioelectronics, vol. 113, pp. 101–107, Aug. 2018.

[14] Y. Zheng, Z. Liu, Y. Jing, J. Li, and H. Zhan, “An acetylcholinesterase biosensor based on ionic liquid functionalized graphene–gelatin-modified electrode for sensitive detection of pesticides,†Sensors and Actuators B: Chemical, vol. 210, pp. 389–397, Apr. 2015.

[15] A. P. Periasamy, Y.-J. Chang, and S.-M. Chen, “Amperometric glucose sensor based on glucose oxidase immobilized on gelatin-multiwalled carbon nanotube modified glassy carbon electrode,†Bioelectrochemistry, vol. 80, no. 2, pp. 114–120, Feb. 2011.

[16] S. Neethirajan, X. Weng, A. Tah, J. O. Cordero, and K. V. Ragavan, “Nano-biosensor platforms for detecting food allergens – New trends,†Sensing and Bio-Sensing Research, vol. 18, pp. 13–30, Apr. 2018.

[17] C. I. L. Justino, A. C. Duarte, and T. A. P. Rocha-Santos, “Critical overview on the application of sensors and biosensors for clinical analysis,†TrAC Trends in Analytical Chemistry, vol. 85, pp. 36–60, Dec. 2016.

[18] F. Fitriyana and F. Kurniawan, “Polyaniline-Invertase-Gold Nanoparticles Modified Gold Electrode for Sucrose Detection,†Indonesian Journal of Chemistry, vol. 15, no. 3, pp. 226–233, 2015.

[19] T. P. Hutapea, Y. Triana, and F. Kurniawan, “Silica Gel / Maja (Aeglemarmelos) Pulp Extract Paste Electrode for Glucose Detection,†p. 3.


[21] Y. Triana, T. P. H. Hutapea, and F. Kurniawan, “The composition of maja leaf extract ( Aegle marmelos ) and silica gel for urea biosensor,†IOP Conference Series: Materials Science and Engineering, vol. 188, p. 012040, Apr. 2017.

[22] Z. Zulkarnain, S. Suprapto, T. Ersam, and F. Kurniawan, “ELECTROCHEMICAL BEHAVIOR OF INSULIN AT SILICA GEL/CHITOSAN/NICKEL NANOPARTICLES PASTE ELECTRODE,†in Proceeding of International Conference On Research, Implementation And Education Of Mathematics And Sciences 2015, 2015.

[23] Z. A. N. Hanani, “Gelatin,†in Encyclopedia of Food and Health, Oxford: Academic Press, 2016, pp. 191–195.

[24] A. A. Mariod and H. Fadul, “Gelatin, source, extraction and industrial applications,†Acta Scientiarum Polonorum Technologia Alimentaria, vol. 12, no. 2, pp. 135–147, 2013.

[25] H. I. Abdullah Amqizal, H. A. Al-Kahtani, E. A. Ismail, K. Hayat, and I. Jaswir, “Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods,†Food Control, vol. 78, pp. 297–303, Aug. 2017.

[26] Y. Demirhan, P. Ulca, and H. Z. Senyuva, “Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication,†Meat Sci., vol. 90, no. 3, pp. 686–689, Mar. 2012.

[27] R. Hafidz, C. M. Yaakob, I. Amin, and A. Noorfaizan, “Chemical and functional properties of bovine and porcine skin gelatin,†International Food Research Journal, vol. 18, pp. 813–817, 2011.

[28] Y. Pranoto, C. M. Lee, and H. J. Park, “Characterizations of fish gelatin films added with gellan and κ-carrageenan,†LWT - Food Science and Technology, vol. 40, no. 5, pp. 766–774, Jun. 2007.

[29] A. F. El Sheikha, N. F. K. Mokhtar, C. Amie, D. U. Lamasudin, N. M. Isa, and S. Mustafa, “Authentication technologies using DNA-based approaches for meats and halal meats determination,†Food Biotechnology, vol. 31, no. 4, pp. 281–315, Oct. 2017.

[30] D. Pradini, H. Juwono, K. A. Madurani, and F. Kurniawan, “A preliminary study of identification halal gelatin using quartz crystal microbalance (QCM) sensor,†vol. 14, no. 3, p. 6, 2018.

[31] E. Emregul et al., “A novel carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase biosensor; electrochemical properties of carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase,†Bioelectrochemistry, vol. 90, pp. 8–17, Apr. 2013.

[32] S. N. Topkaya, “Gelatin methacrylate (GelMA) mediated electrochemical DNA biosensor for DNA hybridization,†Biosensors and Bioelectronics, vol. 64, pp. 456–461, Feb. 2015.

[33] A. Sharma, K. Rawat, H. B. Bohidar, and P. R. Solanki, “Studies on clay-gelatin nanocomposite as urea sensor,†Applied Clay Science, vol. 146, pp. 297–305, Sep. 2017.

[34] E. M. Hamilton, S. D. Young, E. H. Bailey, and M. J. Watts, “Chromium speciation in foodstuffs: A review,†Food Chemistry, vol. 250, pp. 105–112, Jun. 2018.

[35] O. Sunnapu et al., “Rhodamine based effective chemosensor for Chromium(III) and their application in live cell imaging,†Sensors and Actuators B: Chemical, vol. 246, pp. 761–768, Jul. 2017.

[36] Q. Qiao, G. Jin, and X. Hu, “Potentiometric detection of chromium (III) on the carbon fiber electrode modified by n-hexyl calix[4]resorcinarene,†Sensors and Actuators B: Chemical, vol. 160, no. 1, pp. 87–93, Dec. 2011.

[37] S. Li, T. Wei, G. Ren, F. Chai, H. Wu, and F. Qu, “Gold nanoparticles based colorimetric probe for Cr(III) and Cr(VI) detection,†Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 535, pp. 215–224, Dec. 2017.

[38] Badan Pusat Statistik Tarakan, “Laporan Statistik Perikanan Kota Tarakan Tahun 2014.†Kota Tarakan, 2015.

[39] Z. A. N. Hanani, Y. H. Roos, and J. P. Kerry, “Use of beef, pork and fish gelatin sources in the manufacture of films and assessment of their composition and mechanical properties,†Food Hydrocolloids, vol. 29, no. 1, pp. 144–151, Oct. 2012.

[40] N. Cebi, M. Z. Durak, O. S. Toker, O. Sagdic, and M. Arici, “An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins,†Food Chemistry, vol. 190, no. Supplement C, pp. 1109–1115, Jan. 2016.

[41] M. Tominaga, S. Sakamoto, and H. Yamaguchi, “Jungle-Gym Structured Films of Single-Walled Carbon Nanotubes on a Gold Surface: Oxidative Treatment and Electrochemical Properties,†The Journal of Physical Chemistry C, vol. 116, no. 17, pp. 9498–9506, May 2012.

[42] L. Shen, H. Ding, Q. Cao, W. Jia, W. Wang, and Q. Guo, “Fabrication of Ketjen black-high density polyethylene superhydrophobic conductive surfaces,†Carbon, vol. 50, no. 11, pp. 4284–4290, Sep. 2012.

[43] Y. Wang and J. Li, “A carbon nanotubes assisted strategy for insulin detection and insulin proteolysis assay,†Analytica Chimica Acta, vol. 650, no. 1, pp. 49–53, Sep. 2009.

[44] S. Gupta, C. N. Murthy, and C. R. Prabha, “Recent advances in carbon nanotube based electrochemical biosensors,†International Journal of Biological Macromolecules, vol. 108, pp. 687–703, Mar. 2018.

[45] J. A. Kiernan, “Strategies for preventing detachment of sections from glass slides,†Microscopy Today, vol. 99, no. 6, pp. 22–24, 1999.

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How to Cite

P.H. Hutapea, T., ., R., ., M., A. Madurani, K., ., S., & Kurniawan, F. (2019). Milkfish (Chanos Chanos) Gelatin as Biosensor Material for Chromium (III) Detection. International Journal of Engineering & Technology, 7(4.14), 227–231.
Received 2019-02-19
Accepted 2019-02-19
Published 2019-12-24