Optimization of Polystyrene Biodegradation using Response Surface Methodology (RSM) Measured by Simple Colorimetric Method
Keywords:Bacillus aryabhattai, biodegradation, polystyrene, optimization, response surface methodology.
Nearly 280 kilotons of polystyrene (PS) waste being discarded yearly in Malaysia. Banning usage of PS food packaging aims to minimize this â€œwhite pollutionâ€. However, the existing PS waste volume remains the same despite the termination of PS production. Therefore, this study has investigated the optimization of biodegradation of PS using isolated strain Bacillus aryabhattai as an alternative solution to the biodegradation of recalcitrant plastic. The effect of pH and temperature as independent variables were studied at three levels each (pH: 6, 7.5 and 9, temperature: 25 °C, 35 °C and 45 °C) under the designation by three level-factorial and analysed using response surface methodology (RSM) by Design Expert Version 10.0. The biodegradation activity of Bacillus aryabhattai was quantified using simple colorimetric method. Bacillus aryabhattai was inoculated on the dye PS-film and incubated under designed conditions. As a result of biodegradation, the entrapped methylene blue dye was released and measured photometrically. Optimum conditions for the highest reading of blue dye absorbance were obtained at pH 9 and temperature of 25ºC with the desirability of 75.9%. This study provides significant information on the biodegradation activity of Bacillus aryabhattai for future research that can be applied in waste management.
 Emree, K. (2016). Global plastics packaging market to hit $375 billion by 2020. Retrieved March 7, 2017
 Plastemart. (2013). Rise in global demand for polystyrene and expandable polystyrene is led by demand from Asia Pacific. Retrieved March 7, 2017.
 Issam, A. M. (2010). From waste to wealth:A new composite. Universiti Sains Malaysia.
 Hawley-Fedder, R. A., Parsons, M. L., & Karasek, F. W. (1984). Products obtained during combustion of polymers under simulated incinerator conditions. i. polyethylene. Journal of Chromatography A, 314(C), 263â€“273.
 Sielicki, M., Focht, D. D., & Martin, J. P. (1978). Microbial degradation of [14c] polystyrene and 1, 3- diphenylbutane. Canadian Journal of Microbiology, 24(7), 798â€“803.
 Asmita, K., Shubhamsingh, T., Tejashree, S., Road, D. W., & Road, D. W. (2015). Isolation of plastic degrading micro-organisms from soil samples collected at various locations in Mumbai , India. International Research Journal of Environment Sciences. ISSN Int. Res. J. Environment Sci, 4(3), 77â€“85.
 Atiq, N. (2011). Biodegradability of synthetic plastics polystyrene and Styrofoam by fungal isolates. Thesis Submitted in the Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Microbiology. University Islamabad.
 Yang, Y., Yang, J., Wu, W. M., Zhao, J., Song, Y., Gao, L., & Jiang, L. (2015). Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 1. chemical and physical characterization and isotopic tests. Environmental Science and Technology, 49(20), 12080â€“12086.
 ASTM International. standard practice for determining resistance of plastics to bacteria; (1996). ASTM International: West Conshohocken, PA.
 Shinozaki, Y., Watanabe, T., Nakajima-kambe, T., & Kitamoto, H. K. (2013). Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films. Journal of Bioscience and Bioengineering, 115(1), 111â€“114.
 Prescott, L.M., J. P. H. and D. A. K. (1999). Microbiology (4th ed.). ., McGraw-Hill, New York,.
 Daniel, R.M., M.E. Peterson, M.J. Danson, N. C. P. & S.M. Kelly et al. (2010). The molecular basis of the effect of temperature on enzyme activity. Biochem. J, 425, 353â€“360.
 William Gentry Palmer, Jessica Marnay Price, Ashlan & Sullivan, S. H. (2016). The effects of temperature change on respiration rates of superworms (Zophobas morio). Journal of Introductory Biology Investigations, 4(1).
 Hogg, S. (2005). Essential Microbiology. John Wiley and Sons, West Sussex.
 Bisswanger, H. (2014). Enzyme assays. Perspectives in Science, 1(1â€“6), 41â€“55. no. 1â€“6, pp. 41â€“55, 2014.
 Cheba, B. A., Zaghloul, T. I., EL-Mahdy, A. R., & EL-Massry, M. H. (2016). Effect of pH and temperature on Bacillus sp. R2 chitinase activity and stability. Procedia Technology, 22, 471â€“477.
 Dutton, M.V., M. Kathiara, I. M. G. and C. S. E. (1994). Purification and characterization of oxalate decarboxylase from coriolus versicolor. FEMS Microbiol. Lett., 116, 321â€“325.
 Okanlawon, B., Ogunbanwo, S., & Okunlola, A. (2010). Growth of Bacillus cereus isolated from some traditional condiments under different regimens. African Journal of Biotechnology, 9(14), 2129â€“2135.
 Akhavan Sepahy, A., & Jabalameli, L. (2011). Effect of culture conditions on the production of an extracellular protease by bacillus sp. isolated from soil sample of lavizan jungle park. Enzyme Research, 2011(1).
 Carrero-Colon, M., Nakatsu, C. H., Konopka, A., & Carrero-colo, M. (2006). Effect of nutrient periodicity on microbial community dynamics. Applied and Environmental Microbiology, 72(5), 3175â€“3183.
 Jordan, R. C. & Jacobs, E. (1944). The growth of bacteria with a constant food supply. I. Preliminary observations on Bacterium coli. J. Bact., 48, 579.
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