Metagenomic DNA Library: Exploration of Novel Genes Encoding Glycoside Hydrolases

  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract

    Metagenomic is a potential approach to explore novel genes from a reservoir of genes. Metagenomic cDNA library as part of metagenomic approach be able to explore abundant quantity of microbiota genes. Metagenomic library collection was screened by functional and sequence analysis. Functional analysis can be done through induction genes expression substrat. Positive clones can be screened by expression of interest genes. Data of sequence analysis is very important in the process of product identification.



  • Keywords

    Metagenomic, cDNA Library, Glycoside Hydrolase, Digestive Gland, Achatina fulica.

  • References

      [1] Y. Teng, Q. Yin, M. Ding, and F. Zhao, “Purification and characterization of a novel endo-β-1,4-glucanase, AfEG22, from the giant snail, Achatina fulica frussac,” Acta Biochim. Biophys. Sin. (Shanghai)., vol. 42, no. 10, pp. 729–734, 2010.

      [2] A. Baktir, N. A. Arum, Suyanto, and B. Suprijanto, “Enhancing Stability and Purity of Crude Chitinase of Achatina fulica by Crystallization,” Procedia Chem., vol. 18, no. Mcls 2015, pp. 26–30, 2016.

      [3] G. L. Pinheiro et al., “Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica,” Front. Microbiol., vol. 6, no. AUG, pp. 1–15, 2015.

      [4] T. V. Vuong and D. B. Wilson, “Glycoside hydrolases: Catalytic base/nucleophile diversity,” Biotechnol. Bioeng., vol. 107, no. 2, pp. 195–205, 2010.

      [5] A. M. Cardoso et al., “Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes,” PLoS One, vol. 7, no. 11, 2012.

      [6] D. Cowan, Q. Meyer, W. Stafford, S. Muyanga, R. Cameron, and P. Wittwer, Metagenomic gene discovery: Past, present and future, vol. 23, no. 6. 2005.

      [7] S. Pandey et al., “Construction and screening of metagenomic library derived from soil for ??-1, 4-endoglucanase gene,” Biocatal. Agric. Biotechnol., vol. 5, pp. 186–192, 2016.

      [8] B. Singh, T. K. Bhat, N. P. Kurade, and O. P. Sharma, Metagenomics in animal gastrointestinal ecosystem: A microbiological and biotechnological perspective, vol. 48, no. 2. 2008.

      [9] Y. M. Kang, M. K. Kim, J. M. An, M. A. Haque, and K. M. Cho, “Metagenomics of un-culturable bacteria in cow rumen: Construction of cel9E-xyn10A fusion gene by site-directed mutagenesis,” J. Mol. Catal. B Enzym., vol. 113, pp. 29–38, 2015.

      [10] J. Handelsman, “Metagenomics: Application of Genomics to Uncultured Microorganisms,” Microbiol. Mol. Biol. Rev., vol. 69, no. 1, pp. 195–195, 2005.

      [11] S. Voget, H. L. Steele, and W. R. Streit, “Characterization of a metagenome-derived halotolerant cellulase,” J. Biotechnol., vol. 126, no. 1, pp. 26–36, 2006.

      [12] F. Wang, F. Li, G. Chen, and W. Liu, “Isolation and characterization of novel cellulase genes from uncultured microorganisms in different environmental niches,” Microbiol. Res., vol. 164, no. 6, pp. 650–657, 2009.

      [13] M. Allgaier et al., “Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community,” PLoS One, vol. 5, no. 1, 2010.

      [14] M. Y. Yoon et al., “Functional screening of a metagenomic library reveals operons responsible for enhanced intestinal colonization by gut commensal microbes,” Appl. Environ. Microbiol., vol. 79, no. 12, pp. 3829–3838, 2013.

      [15] K. C. Ko, Y. Han, D. E. Cheong, J. H. Choi, and J. J. Song, “Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system,” J. Microbiol. Methods, vol. 94, no. 3, pp. 311–316, 2013.

      [16] Y. Li et al., “Cloning and characterization of a new β-glucosidase from a metagenomic library of rumen of cattle feeding with Miscanthus sinensis.,” BMC Biotechnol., vol. 14, no. 1, p. 85, 2014.

      [17] L. Xiang, A. Li, C. Tian, Y. Zhou, G. Zhang, and Y. Ma, “Identification and characterization of a new acid-stable endoglucanase from a metagenomic library,” Protein Expr. Purif., vol. 102, pp. 20–26, 2014.

      [18] P. Kanokratana, L. Eurwilaichitr, K. Pootanakit, and V. Champreda, “Identification of glycosyl hydrolases from a metagenomic library of microflora in sugarcane bagasse collection site and their cooperative action on cellulose degradation,” J. Biosci. Bioeng., vol. 119, no. 4, pp. 384–391, 2015.

      [19] B. F. Li et al., “Screening and characterization of a novel ruminal cellulase gene (Umcel-1) from a metagenomic library of gayal (Bos frontalis),” J. Integr. Agric., vol. 15, no. 4, pp. 855–861, 2016.

      [20] J. Yun and S. Ryu, “Screening for novel enzymes from metagenome and SIGEX, as a way to improve it,” Microb. Cell Fact., vol. 4, p. 8, 2005.

      [21] Y. Teng, Q. Yin, and F. Zhao, “Purification and characterization of a novel endo-b-1 , 4-glucanase , AfEG22 , from the giant snail , Achatina fulica Frussac,” Acta Biochim. Biophys. Sin, vol. 42, no. 10, pp. 729–734, 2010.

      [22] M. Ueda, A. Ito, M. Nakazawa, K. Miyatake, M. Sakaguchi, and K. Inouye, “Cloning and expression of the cold-adapted endo-1,4-β-glucanase gene from Eisenia fetida,” Carbohydr. Polym., vol. 101, no. 1, pp. 511–516, 2014.

      [23] K. Sakamoto and H. Toyohara, “Molecular cloning of glycoside hydrolase family 45 cellulase genes from brackish water clam Corbicula japonica,” Comp. Biochem. Physiol. - B Biochem. Mol. Biol., vol. 152, no. 4, pp. 390–396, 2009.

      [24] S. M. Linton et al., “A glycosyl hydrolase family 16 gene is responsible for the endogenous production of β-1,3-glucanases within decapod crustaceans,” Gene, vol. 569, no. 2, pp. 203–217, 2015.

      [25] T. E. Brown, Gene cloning and DNA Analysis, an introduction, Third. Manchester, 2010.

      [26] and G. R. Struble, J.M., Handke P, “Genome Sequen Databases: Genomic, Construction of libraries,” The Desk Encyclopedia of Mocrobiology. Oxford: Academic Press, 2009.

      [27] P. H. P. H. Faix, M. A. M. A. Burg, M. M. Gonzales, E. P. E. P. Ravey, A. A. Baird, and D. D. Larocca, “Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection.,” Biotechniques, vol. 36, no. 6, pp. 1018–1019, 2004.

      [28] L. . Kleinsmith and V. . Kish, Principles of Cell and Molecular Biology. New York: HarperCollins College Publisher, 2002.

      [29] A. Munshi, DNA SEQUENCING – METHODS AND Edited by Anjana Munshi. 2012.

      [30] F. M. Ausubel et al., Current Protocols in Molecular Biology Current Protocols in Molecular Biology. 2003.




Article ID: 27362
DOI: 10.14419/ijet.v7i4.7.27362

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