The Effects of Ketapang (Terminalia catappa) Bark Crude Extract on Inhibition of Aeromonas hydrophila Growth and Blood Cells of the Infected Carp (Cyprinus carpio)

  • Authors

    • Maftuch .
    • Heny Suprastyani
    • Ellana Sanoesi
    • Gede Agus Andika Kresna Putra
    • Mirsa Ferdian Putra
    https://doi.org/10.14419/ijet.v8i1.9.26671
  • Ketapang bark, Aeromonas hydrophila, carp, erythrocytes, leukocytes

  • Research aimed to determine the effect of ketapang (Terminalia catappa) bark crude extract (KBCE) on inhibition of Aeromonas hydrophila growth and blood cells of the infected carp (Cyprinus carpio). Research was conducted by completely randomized design for both in vitro and in vivo. There were four (750, 850, 950, and 1,050 ppm) and three treatments (730, 750, and 770 ppm) were used for in vitro and in vivo experiments respectively. Both experiments were replicated three times. Diameter of inhibitory zone on A. hydrophila growth was main variable in experiments. Data were analyzed by analysis of variation (and LSD. based on in vitro, 750, 850, 950 and 1,050 ppm KBCE produced the average inhibitory zone diameter of 8.97, 10.21, 11.69 and 13.89 mm respectively.  On in vivo, erythrocytes were 224.67x10, 332.67x10, and 417.00x10 cells/mm for 730, 750 and 770 ppm KBCE respectively. In case of leukocytes were 735.89x10, 684.78x10, and 652.67x10 cell/mm for 730, 750 and 770 ppm KBCE respectively. In addition, based on measurement of water quality, water had 24.0 – 25.9ï‚°C of temperature, 7.0 – 7.7 of pH, and 3.9 – 4.8 mg/L of DO.

     

     

  • References

    1. [1] Bhatnagar, A. and Devi, P. 2013. Water quality guidelines for the management of pond fish culture. International Journal of Environmental Sciences. Vol 3, No. 6.

      [2] Bondad MG, Subasinghe RP, and Arthur JR. 2005. Disease and health management in Asian aquaculture. Veterinary Parasito-logy. 132 (3-4): 249–272

      [3] Cipriano R.C. 2001. Aeromonas hydrophila and motile Aeromonad septicemias of fish. Fish Dis Leaflet 68, United States Department of the Interior fish and wildlife service, Division of Fishery research. Washington, DC. pp.1-25.

      [4] Gusrina. 2008. Aquaculture. Dorectorate of Vocational High School. Directorate of Technical and Vocational Education, Directorate General for Primary and Secondary Education, Ministry of National Education. Jakarta. Vol. 3. (In Indonesia).

      [5] Harborne, A.J. 1998. Phytochemical Methods: a Guide to Modern Tecniques of Plant Analysis. Springer

      [6] Ismaini, L. 2007. Study on Activities and Analysis of Chemical Compounds Antibacterial Picung Seed Extract (Pangium edule Reinw). Thesis. Faculty of Science, Graduate School of the University of Indonesia. (In Indonesia).

      [7] Kapoor, B.G. and Khanna, B. 2004. Ichthyology Handbook. Page 301

      [8] Meyer FP. 1991. Aquaculture disease and health management. Journal of Animal Science. 69(10): 4201–4208.

      [9] Ministry of Marine Affairs and Fisheries (MMAF). (2015). The submission of meeting of the Ministry of Marine Affairs and Fisheries with the House of Representatives. Senayan. Jakarta.

      [10] Maftuch, M.H. Toban, Y. Risjani. (2012). Administration of marine algae (Gracilaria verrucosa) immunostimulant enhances some innate immune parameters in black tiger shrimp (Penaeus monodon Fabricus) against Vibrio harveyi infection. Journal of Applied Sciences Research, 8(2): 1052-1058, 2012

      [11] Maftuch, E Prasetio, Murachman, A Sudianto, M Rozik, R Nurdiyani, E Sanusi, H Nursyam, F Fariedah, Marsoedi. (2013). Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus. SpringerPlus 2013, 2:432,http://www.springerplus.com/content/2/1/432.

      [12] Masuda T, Yonemori S, Oyama Y, Takeda Y, Tanaka T, Andoh T. 1999. Evaluation of the antioxidant activity of environmental plants: Activity of the leaf extracts from seashore plants. J Agric Food Chem. 47:1749–54.

      [13] Monera, D. dan Simon A. 2008. The Impact of Toxic Heavy Metals on The Hematological Parameters in Common Carp (Cyprinus carpio). Journal Environment. 6 (1): 23-28.

      [14] Mones, R. A. 2008. Description of Blood Cells in Carp (Cyprinus carpio) Majalaya Strain Originating from the Regional Ciampea. Thesis. IPB. Bogor.

      [15] P. Neelavathi, P. Venkatalakshmi, P. Brindha. 2013. Antibacterial Activities Of Aqueous And Ethanolic Extracts Of Terminaliacatappa Leaves and Bark Against Some Pathogenic Bacteria. International Journal of Pharmacy and PharmaceuticalSciences, Vol. 5; 0975-1491

      [16] Pelczar, M. J., and E. C. S. Chan. 1986. Microbiology. Published by McGraw-Hill

      [17] Riskitavani, D.V and K.I Purwani. 2013. Study of Bioherbisida Leaf Extract Ketapang (Terminalia catappa) against nutgrass (Cyperus rotundus). Department of Biology, Faculty of Mathematics and Natural Sciences, the Institute of Technology (ITS). Page: 21 -22. (In Indonesia).

      [18] Shak JR, Whitaker JA, Ribner BS, Burd EM. 2011 Aminoglycoside- Resistant Aeromonas hydrophila as Part of a Polymicrobial Infection Following a Traumatic Fall into Fresh Water. J Clin Microbiol 49: 1169-1170.

      [19] Snieszko, S. F. 1973. Recent advances in scientific knowledge and developments pertaining to diseases of fishes. Advances in Veterinary Science and Comparative Medicine 17:291–314.

      [20] Suhermanto, A, S. Andayani and Maftuch. 2011. Giving of Phenol Total from Sea Cucumber (Holothuria scabra) to Improve Leukocyte and Differential Leukocyte of Carp (Cyprinus carpio) infected by Aeromonas hydrophila. Journal of Marine. 4 (2): 49-56. (In Indonesia).

      [21] Sumino., A. Supriyadi and Wardiyanto. 2013. Effectiveness of Leaf Extract Ketapang (Terminalia catappa L.) for the treatment of infections Aeromonas salmonicida on Catfish (Pangasioniodon hypophthalmus). Department of Aquaculture, University ofLampung. JSV: 31 (1). 79-88. (In Indonesia).

      [22] Takashi. F. and T. Hibiya. 1995. An Atlas of Fish Histology Normal and Phatology Feature. Tokyo Kodansha Ltd. 108 hlm.

      [23] Thomsons, A., and Evans. 2006) The Potential of Terminalia catappa (Almond) and Hyphaene thebaica (Dum Palm) Fruits as Raw Material for Livestock Feed. African Journal of Biotechnology. 7 (24): 4576-4580 pp.

      [24] Turutoglu H., Ercelik S., Corlu M. 2005. Aeromonas hydrophila-associated skin lesions and septicaemia in a Nile crocodile (Crocodylus niloticus). J S Afr Vet Ass, 76, 40-42.

      [25] Volk, W.A dan M.F. Wheeler. 1984. Basic Microbiology. Fifth Edition. Erlangga Publisher. Jakarta. 343 pages

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    ., M., Suprastyani, H., Sanoesi, E., Agus Andika Kresna Putra, G., & Ferdian Putra, M. (2019). The Effects of Ketapang (Terminalia catappa) Bark Crude Extract on Inhibition of Aeromonas hydrophila Growth and Blood Cells of the Infected Carp (Cyprinus carpio). International Journal of Engineering & Technology, 8(1.9), 287-292. https://doi.org/10.14419/ijet.v8i1.9.26671