Study Identification of Bioactive Compounds from Fractional Extracts of Cornlettes Using LC-TOF/MS
Keywords:Antioxidant activity, Cornlettes, Phenolic, Phytochemicals, LC-MS
Cornlettes or baby corn is a type of vegetable. It can be easily found in Asian cuisines. Since previous reports indicated that the crude extracts of cornlettes showed some significant antioxidative scavenging properties, this vegetable may contain certain functional bioactive compounds. This study aimed to identify bioactive constituents in the fractional extract of local Malaysian cornlettes. The aqueous-alcoholic extract of cornlettes was fractioned with hexane and ethyl acetate, subsequently. The LC-MS system was used to elucidate different types of functional compounds from the ethyl acetate fraction. Both crude and the fractions were evaluated for total phenolic content and antioxidant activities. The LC-MS analysis of ethyl acetate fraction showed that it contains six phenolic compounds, four carboxylic acids and one for each of alkaloid, amino acid, ethyl ester and adenine. The ethyl acetate fraction exhibited the most significant DPPH radical-scavenging activity and the highest amount of total phenolic compounds. In summary, cornlettes may provide a rich source of phenolic compounds and functional nutrients including essential fatty acids.
 Asha Tukappa, N.K. and R.L. Londonkar, Standardization of extraction process for Rumex vesicarius L. International Journal of Scientific & Engineering Research, 2014. 5(8): p. 1061-1063.
 Joana Gilâ€ChÃ¡vez, G., et al., Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview. Comprehensive Reviews in Food Science and Food Safety, 2013. 12(1): p. 5-23.
 Reidah, A.I.M.M., Characterization of phenolic compounds in highly-consumed vegetable matrices by using advanced analytical techniques. 2013, University of Granada.
 Chu, Y.-F., et al., Antioxidant and antiproliferative activities of common vegetables. Journal of agricultural and food chemistry, 2002. 50(23): p. 6910-6916.
 Liu, R.H., Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. The American journal of clinical nutrition, 2003. 78(3): p. 517S-520S.
 Hooda, S. and A. Kawatra, Nutritional evaluation of baby corn (Zea mays). Nutrition & Food Science, 2013. 43(1): p. 68-73.
 Zin, C.A.J.C.M., S.D. Robert, and W.R.W. Ishak, Effect of Biscuits and Muffins Added with Cornlettes Powder on the Glycemic Responses of Healthy Individuals. Food and Nutrition Sciences, 2014. 5(22): p. 2195-2202.
 Mei, H.Y., Antioxidant capacity, total phenolic and total flavonoid content in young corn ear and cornsilk of different varieties. 2014, Universiti Sains Malaysia.
 Nurraihana, H., et al. Optimisation extraction procedure and identification of phenolic compounds from fractional extract of corn silk (Zea mays hair) using LC-TOF/MS system. Journal of Food Measurement and Characterization, 2018. DOI: http://link.springer.com/10.1007/s11694-018-9799-z.
 Mariod, A.A., et al., Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake. Food Chemistry, 2009. 116(1): p. 306-312.
 11. Ayaz, M., et al., Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L. BMC complementary and alternative medicine, 2014. 14(1): p. 1.
 Karimi, A. and M.-T. Moradi, Total phenolic compounds and in vitro antioxidant potential of crude methanol extract and the correspond fractions of Quercus brantii L. acorn. J HerbMed Pharmacol, 2015. 4: p. 35-9.
 Ow, Y.-Y. and I. Stupans, Gallic acid and gallic acid derivatives: effects on drug metabolizing enzymes. Current drug metabolism, 2003. 4(3): p. 241-248.
 Takayanagi, Y., Hot Water Extract of Bark of Nikko Maple (Acer nikoense) Induces Apoptosis in Leukemia Cells. Biological and Pharmaceutical Bulletin 1999. 22(4): p. 378-381.
 Rayan, P., et al., Phytochemical analysis of Tasmannia lanceolata extracts and inhibition of Giardia duodenalis proliferation. Pharmacognosy Journal, 2016. 8(3).
 University of Maryland Medical Center. Alpha-linolenic acid. 2013 8 January 2017]; Available from: http://umm.edu/health/medical/altmed/supplement/alphalinolenic-acid.
 Funayama, S., G.-R. Zhang, and S. Nozoe, Kukoamine B, a spermine alkaloid from Lycium chinense. Phytochemistry, 1995. 38(6): p. 1529-1531.
 Liu, X., et al., Kukoamine B, a novel dual inhibitor of LPS and CpG DNA, is a potential candidate for sepsis treatment. British journal of pharmacology, 2011. 162(6): p. 1274-1290.