Prevalence of Panton-Valentine Leukocidin Staphylococcus aureus among Patients and Healthcare Workers in Malaysian Public Hospital


  • Syahirah Samsudin
  • Hassanain Al-Talib
  • Zaini Mohd-Zain
  • Alyaa Al-Khateeb
  • Norliana Dalila Mohamad Ali
  • . .





methicillin-resistance S. aureus (MRSA), methicillin sensitive S. aureus (MSSA), polymerase chain reaction (PCR), PVL, S. aureus


Staphylococcus aureus carrying Panton-Valentine leukocidin (PVL) has become a worldwide threat to public health. Recent reports showed an increasing numbers of Staphylococcus aureus infections caused by PVL-positive organisms worldwide which explain the need to carry out this study in Malaysia. The objective of this study was to determine the prevalence of PVL-positive Staphylococcus aureus among patients and healthcare workers in Malaysian Public Hospital. Collectively, 230 swabs were collected from the anterior nares of inpatients and healthcare workers at Sungai Buloh Hospital. The isolates were identified and characterized using conventional and molecular methods. From 230 samples collected, 23% (53/230) were detected as Staphylococcus aureus isolates. Out of the 53 Staphylococcus aureus isolates, 2 (3.8%) were found to be PVL-positive Staphylococcus aureus (from patient’s sample) and the remaining 51 (96.2%) isolates were PVL negative Staphylococcus aureus. Out of 53 Staphylococcus aureus isolates, 40 (75.5%) were methicillin-sensitive and 13 (24.5%) were methicillin-resistance Staphylococcus aureus. The finding of the prevalence of PVL-positive Staphylococcus aureus among patients and healthcare workers will provide awareness to the hospital authority to take action for prevention and control.




[1] Jenkins, B. An Diep, T. Mai, N. Vo, P. Warrener, J. Suzich, C. Kendall Stover, and B. Sellman, “Differential Expression and Roles of Staphylococcus aureus Virulence Determinants during Colonization and Disease,†MBio, vol. 6, no. 1, pp. 1–10, 2015.

[2] J. Kluytmans, A. van Belkum, and H. Verbrugh, “Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks.,†Clin. Microbiol. Rev., vol. 10, no. 3, pp. 505–20, 1997.

[3] G. L. Archer, “Staphylococcus aureus: A well-armed pathogen,†Clin. Infect. Dis., vol. 26, no. 5, pp. 1179–1181, 1998.

[4] H. Wertheim, and D. Melles, “Review The role of nasal carriage in Staphylococcus aureus infections,†vol. 5, no. December, pp. 751–762, 2005.

[5] Adler, V. Temper, C. S. Block, N. Abramson, and A. E. Moses, “Panton-Valentine leukocidin-producing Staphylococcus aureus [1],†Emerg. Infect. Dis., vol. 12, no. 11, pp. 1789–1790, 2006.

[6] J. Maier, H. Melzl, U. Reischl, I. Drubel, W. Witte, N. Lehn, H. Linde, “Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus in Germany associated with travel or foreign family origin,†Eur. J. Clin. Microbiol. Infect. Dis., vol. 24, no. 9, pp. 637–639, 2005.

[7] V. Chini, E. Petinaki, A. Foka, S. Paratiras, G. Dimitracopoulos, and I. Spiliopoulou, “Spread of Staphylococcus aureus clinical isolates carrying Panton-Valentine leukocidin genes during a 3-year period in Greece,†Clin. Microbiol. Infect., vol. 12, no. 1, pp. 29–34, 2006.

[8] M. Haarman and J. Knol, “Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula,†Appl Env. Microbiol, vol. 72, no. 4, pp. 2359–2365, 2006.

[9] (2018). NRBSC – Nture responsible center. [online] Available at: [Accessed 16 May. 2017].

[10] Anon, (n.d.). NCBI Blast - BLAST: Basic Local Alignment Search Tool. [online] Available at: [Accessed 15 May. 2017].

[11] H. Al-Talib, H. Hasan, C. Y. Yean, S. M. Al-Ashwal, and M. Ravichandran, “Fatal necrotizing pneumonia caused by panton-Valentine leukocidin-producing hospital-acquired Staphylococcus aureus: A case report,†Jpn. J. Infect. Dis., vol. 64, no. 1, pp. 58–60, 2011.

[12] V. K. Neela, G. R. Ehsanollah, S. Zamberi, A. Van Belkum, and N. S. Mariana, “Prevalence of Panton-Valentine leukocidin genes among carriage and invasive Staphylococcus aureus isolates in Malaysia,†International Journal of Infectious Diseases, vol. 13, no. 3. 2009.

[13] Rayner and W. J. Munckhof, “Antibiotics currently used in the treatment of infections caused by Staphylococcus aureus,†Internal Medicine Journal, vol. 35, no. SUPPL. 2. 2005.

[14] Betriu, I. Rodriguez-Avial, B.A Sanchez, M. Gomez, J. Ãlvarez, J Picazo, J. Aznar, E. Bouza, R. Cisterna, J. De Lomas, J. Moya, J. García-Rodríguez, A. Guerrero, M. De Anta, E. Perea, M. De la Rosa, and J. Ruiz, “In vitro activities of tigecycline (GAR-936) against recently isolated clinical bacteria in Spain,†Antimicrob. Agents Chemother., vol. 46, no. 3, pp. 892–895, 2002.

[15] F. Liaqat, A. Sheikh, J. Nazir, T. Hussain, M. Rabbani, A. Shaheen, and J. Muhammad, “Report-Isolation identification and control of vancomycin resistant Staphylococcus aureus,†Pak. J. Pharm. Sci., vol. 28, no. 3, pp. 997–1004, 2015.

[16] S. M. Edslev, M.-L. Clausen, T. Agner, M. Stegger, and P. S. Andersen, “Genomic analysis reveals different mechanisms of fusidic acid resistance in Staphylococcus aureus from Danish atopic dermatitis patients,†J. Antimicrob. Chemother., no. December, pp. 1–6, 2017.

[17] X. Liu, S. Zhang, and B. Sun, “SpoVG regulates cell wall metabolism and oxacillin resistance in methicillin-resistant Staphylococcus aureus strain N315,†Antimicrob. Agents Chemother., vol. 60, no. 6, pp. 3455–3461, 2016.

[18] H. Al-Talib, A. Al-Khateeb, and H. Hassan, “Antimicrobial Resistance of Staphylococcus aureus Isolates in Malaysian Tertiary Hospital,†vol. 22, no. November, pp. 0–3, 2015.

[19] M. C. Enright, N. P. J. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt, “Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus,†J. Clin. Microbiol., vol. 38, no. 3, pp. 1008–1015, 2000.

[20] S. A. Havaei, S. Ohadian Moghadam, M. R. Pourmand, and J. Faghri, “Prevalence of genes encoding bi-component leukocidins among clinical isolates of methicillin-resistant Staphylococcus aureus,†Iran. J. Public Health, vol. 39, no. 1, pp. 8–14, 2010.

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How to Cite

Samsudin, S., Al-Talib, H., Mohd-Zain, Z., Al-Khateeb, A., Dalila Mohamad Ali, N., & ., . (2018). Prevalence of Panton-Valentine Leukocidin Staphylococcus aureus among Patients and Healthcare Workers in Malaysian Public Hospital. International Journal of Engineering & Technology, 7(4.42), 194–196.
Received 2019-01-11
Accepted 2019-01-11
Published 2018-12-29