Biodegradation of Quinoline by Hydrogel Beads of Activated Sludge in the Air-Lift Bioreactor

  • Authors

    • Haneen Ahmed Khudhair
    • . .
    2018-12-13
    https://doi.org/10.14419/ijet.v7i4.37.23615
  • activated sludge, air-lift bioreactor, biodegradation, calcium alginate, hydrogel beads, poly vinyl-alcohol, and quinoline.

  • Quinoline is a nitrogen heterocyclic compound (NHC) with a molecular formula of C9H7N. Microbial degradation of quinoline occurs under both aerobic and anaerobic conditions. In this study, the aerobic biodegradation of quinoline was investigated with activated sludge which was taken from a municipal sewage wastewater treatment plant in the air-lift bioreactor (ALBR).The activated sludge was entrapped in poly-vinyl-alcohol-calcium alginate (PVA-Ca alginate) hydrogel beads. The optimal conditions for microbial cells entrapment, such as Ca-alginate concentration, biomass concentration in the hydrogel bead and bead size, were determined with concerning to improve the quinoline degradation rate. Also, the initial quinoline concentration was determined. The optimum temperature and initial pH for quinoline degradation were 30°C and pH 7–8, respectively. During the biodegradation process, the culture broth became yellow and brown in turn, which indicated that several intermediates were generated. The results showed that the diversity of microbial cells improves and accelerates the quinoline biodegradation. The repeated use of the hydrogel beads for quinoline degradation was performed and the results revealed that the beads were active and intact up to 4 successive cycles without breakage or loss their stability in the continuous mode. Thus, (PVA-Ca alginate) hydrogel beads have great potential to be a matrix for the cell immobilization in quinoline biodegradation. The various initial concentrations of quinoline (50, 100, 250, and 500mg/L) were completely degraded in batch-mode experiments; under optimal conditions: PVA conc., 80%w/v; calcium alginate conc., 20%w/v; initial biomass concentration the hydrogel bead, 3mL / 10 mL of gel solution; and the hydrogel bead size, about 3mm in diameter, at different time intervals (4, 6, 10, and 14h), respectively.

     

     


     

  • References

    1. [1] Ahmad, M., Liu, S., Mahmood, N., Mahmood, A., Ali, M., Zheng, M., & Ni, J. (2017). Synergic adsorption–biodegradation by an advanced carrier for enhanced removal of high-strength nitrogen and refractory organics. ACS applied materials & interfaces, 9(15), 13188-13200.

      [2] Aislabie, J., Bej, A. K., Hurst, H., Rothenburger, S., & Atlas, R. M. (1990). Microbial degradation of quinoline and methylquinolines. Applied and environmental microbiology, 56(2), 345-351.

      [3] Ali, M., & Okabe, S. (2015). Anammox-based technologies for nitrogen removal: advances in process start-up and remaining issues. Chemosphere, 141, 144-153.

      [4] An, M., & Lo, K. V. (2001). Activated sludge immobilization using the PVA-alginate-borate method. Journal of Environmental Science and Health, Part A, 36(1), 101-115.

      [5] Bai, Y., Sun, Q., Zhao, C., Wen, D., & Tang, X. (2010). Bioaugmentation treatment for coking wastewater containing pyridine and quinoline in a sequencing batch reactor. Applied microbiology and biotechnology, 87(5), 1943-1951.

      [6] Bajpai, S. K., & Sharma, S. (2004). Investigation of swelling/degradation behaviour of alginate beads crosslinked with Ca2+ and Ba2+ ions. Reactive and Functional Polymers, 59(2), 129-140.

      [7] Doria-Serrano, M. C., Ruiz-Trevino, F. A., Rios-Arciga, C., Hernandez-Esparza, M., & Santiago, P. (2001). Physical characteristics of poly (vinyl alcohol) and calcium alginate hydrogels for the immobilization of activated sludge. Biomacromolecules, 2(2), 568-574.

      [8] Eisentraeger, A., Brinkmann, C., Hollert, H., Sagner, A., Tiehm, A., & Neuwoehner, J. (2008). Heterocyclic compounds: toxic effects using algae, daphnids, and the Salmonella/microsome test taking methodical quantitative aspects into account. Environmental toxicology and chemistry, 27(7), 1590-1596.

      [9] Felczak, A., Zawadzka, K., & Lisowska, K. (2014). Efficient biodegradation of quinolone–Factors determining the process. International Biodeterioration & Biodegradation, 96, 127-134.

      [10] Fetzner, S., Tshisuaka, B., Lingens, F., Kappl, R., & Hüttermann, J. (1998). Bacterial degradation of quinoline and derivatives—pathways and their biocatalysts. Angewandte Chemie International Edition, 37(5), 576-597.

      [11] Gosmann, B., & Rehm, H. J. (1986). Oxygen uptake of microorganisms entrapped in Ca-alginate. Applied microbiology and biotechnology, 23(3-4), 163-167.

      [12] Jianlong, W., Liping, H., Hanchang, S., & Yi, Q. (2001). Biodegradation of quinoline by gel immobilized Burkholderia sp. Chemosphere, 44(5), 1041-1046.

      [13] Jianlong, W., Xiangchun, Q., Liping, H., Yi, Q., & Hegemann, W. (2002). Microbial degradation of quinoline by immobilized cells of Burkholderia pickettii. Water Research, 36(9), 2288-2296.

      [14] Kim, H. Y., Lee, O. M., Kim, T. H., & Yu, S. (2015). Enhanced biodegradability of pharmaceuticals and personal care products by ionizing radiation. Water Environment Research, 87(4), 321-325.

      [15] Lin, Q., & Jianlong, W. (2010). Biodegradation characteristics of quinoline by Pseudomonas putida. Bioresource Technology, 101(19), 7683-7686.

      [16] Lopes, T. J., & Furlong, E. T. (2001). Occurrence and potential adverse effects of semivolatile organic compounds in streambed sediment, United States, 1992–1995. Environmental toxicology and chemistry, 20(4), 727-737.

      [17] Sun, Q., Bai, Y., Zhao, C., Xiao, Y., Wen, D., & Tang, X. (2009). Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain. Bioresource technology, 100(21), 5030-5036.

      [18] Wang, J. L., Wu, W. Z., & Zhao, X. U. A. N. (2004). Microbial degradation of quinoline: kinetics study with Burkholderia picekttii. Biomedical and Environmental Sciences, 17(1), 21-26.

      [19] Wang, L., Li, Y., & Duan, J. (2014). Biodegradation of 2-methylquinoline by Klebsiella pneumoniae TJ-A isolated from acclimated activated sludge. Journal of Environmental Science and Health, Part A, 49(1), 27-38.

      [20] Xu, P., Ma, W., Han, H., Hou, B., & Jia, S. (2015). Biodegradation and interaction of quinoline and glucose in dual substrates system. Bulletin of environmental contamination and toxicology, 94(3), 365-369.

      [21] Zhang, L. S., Wu, W. Z., & Wang, J. L. (2007). Immobilization of activated sludge using improved polyvinyl alcohol (PVA) gel. Journal of environmental sciences, 19(11), 1293-1297

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    Ahmed Khudhair, H., & ., . (2018). Biodegradation of Quinoline by Hydrogel Beads of Activated Sludge in the Air-Lift Bioreactor. International Journal of Engineering & Technology, 7(4.37), 53-56. https://doi.org/10.14419/ijet.v7i4.37.23615