Isolation and Characterization of Bacteria from Earthworms’ Intestines

  • Authors

    • Shalyda Md Shaarani
    • Yap Shu Jia
    • Zatul Iffah Mohd Arshad
    • Rohaida Che Man
    • Siti Kholijah Abdul Mudalip
    • Siti Zubaidah Sulaiman
  • Bacteria, Intestines, Nitrogen Fixing, Phosphorus Solubilizing, Vermicomposting
  • Vermicomposting; breaking down of organic material by earthworms that feed on wastes and converts them to soil-like mass and liquid, is an alternative to reduce waste into the environment. Nitrogen fixing and Phosphorus solubilizing bacteria in the earthworms’ intestines are important in promoting plant growth by increasing the Nitrogen (N) and Phosphorus (P) uptake when used as biofertilizers. The objectives of this study are to isolate, identify and characterize N-fixing and P-solubilizing bacteria from the earthworms’ intestines. The preparation of vermibeds included the introduction of leachate taken from Sungai Ikan Landfill, Kuala Terengganu. Standard serial dilution procedure was performed to isolate the microorganisms. Next, identification and characterization of bacteria were conducted via gram staining and bacteria morphological characteristic studies. Qualitative screening of N-fixing bacteria was performed by Hach Method 8039 while screening of P-solubilizing bacteria used Pikovskaya’s Agar containing insoluble tri-calcium phosphate (TCP). Six isolates, (A1, A2, B1, B2, C1 and D1) were successfully isolated. However, only single colony of A1, A2, B1 and B2 were obtained. All were gram negative and bacilli except for A1. A2 showed the highest nitrogen fixing and phosphate solubilizing activity though with the nitrate content (148.6 mg/L) and high phosphate solubilization efficiency (10.6).

  • References

    1. [1] Periathamby A, Hamid FS, K Khidzir (2009), Evolution of solid waste management in Malaysia: Impacts and implications of the solid waste bill, J. Mater. Cycles Waste Manag. 11, 96–103, available online: doi:10.1007/s10163-008-0231-3.

      [2] Kamaruddin MA, Yusoff MS, Aziz HA, Hung YT (2015), Sustainable treatment of landfill leachate. Appl. Water Sci. 5,113–126, available online: doi:10.1007/s13201-014-0177-7.

      [3] Wiszniowski J, Robert D, Surmacz-Gorska J, Miksch K, Weber JV (2006), Landfill leachate treatment methods: A review. Environ. Chem. Lett. 4, 51–61, available online: doi:10.1007/s10311-005-0016-z.

      [4] Marangon KC, Henrique E, Silva DF, Lucas K, De I, Rodrigues S (2014), Impact of Vermicomposting products in soil fertility of degraded Brazilian Cerrado and the perspective to minimize water pollutants release.

      [5] Revolution G, Earthworm T, District E, Prabha L (2006), 4. results and discussion,.

      [6] Pfiffner L (2014), Earthworms – Architects of fertile soils. Their significance and recommendations for their promotion in agriculture. Tech. Guid. Earthworms 9.

      [7] Hussain N, Singh A, Saha S, Kumar MVS, Bhattacharyya P, Bhattacharya SS (2016), Excellent N-fixing and P-solubilizing traits in earthworm gut-isolated bacteria: A vermicompost based assessment with vegetable market waste and rice straw feed mixtures. Bioresour. Technol 222, 165–174, available online: doi:10.1016/j.biortech.2016.09.115.

      [8] Gislin D, Sudarsanam D, Raj GA, Baskar K (2018), Antibacterial activity of soil bacteria isolated from Kochi, India and their molecular identification. J. Genet. Eng. Biotechnol, available online: doi:10.1016/j.jgeb.2018.05.010.

      [9] Karpagam T, Nagalakshmi PK (2014), Original esearch Article Isolation and characterization of Phosphate Solubilizing Microbes from Agricultural soil. Int. J. Curr. Microbiol. Appl. Sci 3, 601–614.

      [10] Sharma S, Kumar V, Tripathi RB (2011), Isolation of Phosphate Solubilizing Microorganism (PSMs) From Soil. J. Microbiol. Biotechnol. Res. Sch. Res. Libr. 1, 90–95.

      [11] Khan HR, Rahman M (2008), Enumeration , Isolation and Identification of Nitrogen-Fixing Bacterial Strains at Seedling Stage in Rhizosphere of Rice Grown in Non-Calcareous Grey Flood Plain Soil of Bangladesh, 13, 97–101.

      [12] Maheswari NU, Sudha S (2013), Enumeration and detection of phosphate solubilizing bacteria from the gut of earthworm varieties. J. Chem. Pharm. Res. 5, 264–267.

      [13] Vollmer W, Höltje J, Ho J (2004), The Architecture of the Murein ( Peptidoglycan ) in Gram-Negative Bacteria : Vertical Scaffold or Horizontal Layer ( s )? MINIREVIEW The Architecture of the Murein ( Peptidoglycan ) in Gram-Negative Bacteria : Vertical Scaffold or Horizontal Layer ( s )?†, 186, 5978–5987. available online: doi:10.1128/JB.186.18.5978.

      [14] Sivasankari B, Anandharaj M (2014), Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources, available online: doi:10.1155/2014/248591.

      [15] Kadiri DD, Gorle N, Varada K, Peetala R, Peela S (2013), Isolation , Screening and Identification of Phosphate Solubilizing Bacteria From Different Regions of, 4, 518–526.

      [16] Shanti RI, Rekha S, Anitha AA (2012), Analysis of Nitrogen and Phosphate in Enriched and Non Enriched, J. Environ. Res. Dev. 7, 899–904.

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  • How to Cite

    Shaarani, S. M., Jia, Y. S., Arshad, Z. I. M., Man, R. C., Mudalip, S. K. A., & Sulaiman, S. Z. (2018). Isolation and Characterization of Bacteria from Earthworms’ Intestines. International Journal of Engineering & Technology, 7(4.30), 322-325.