BSA /anti BSA Interaction Study using a Novel Modular Surface Plasmon Resonance (m-SPR) Device

  • Authors

    • Wan Mohd Azwady Wan Ahamad
    • Dzaraini Kamarun
    • Mohd Kamil Abd Rahman
    2018-11-27
    https://doi.org/10.14419/ijet.v7i4.18.21830
  • Surface plasmon resonance, biosensors, portable, biomolecular interaction

  • A novel modular surface plasmon resonance (SPR) device fabricated in-house was tested for its function and linearity for biomolecular interaction using BSA and α-BSA as model biomolecules. The sensor surface was initially saturated with BSA molecules and their interactions with α-BSA molecules were then monitored over various concentrations of α-BSA. The newly developed modular SPR biosensor was able to detect biomolecular interactions between BSA and α-BSA at α-BSA with good linearity. Stabilization of the interactions was achieved within 10 minutes of monitoring time.

     

  • References

    1. [1] E. Kretschmann and H. Raether (1968), Radiative decay of non-radiative surface plasmons excited by light. Zeithschrift für Naturforschung 23, 2135–2136.

      [2] I. Pockrand, J. D. Swalen, J. G. Gordon, and M. R. Philpott (1978), Surface plasmon spectroscopy of organic monolayer assemblies. Surface Science. 74, 237–244.

      [3] B. O. Liedberg (1983), Surface plasmon resonance biosensing* for gas detection and. Sensors and actuators 4, 299–304.

      [4] C. R. Lowe (1985), An introduction to the concepts and technology of biosensors. Biosensors 1, 3–16.

      [5] B. Rothenhäusler and W. Knoll (1988), Surface–plasmon microscopy. Nature 332, 615.

      [6] W. Na, M. Kang, D. Jang, and S. Shin (2018), Miniaturized surface plasmon resonance biosensor with vacuum-driven hydrodynamic focusing. Sensors and Actuator B: Chemical 254, 64–71.

      [7] B. A. Prabowo, L.-C. Su, Y.-F. Chang, H.-C. Lai, N.-F. Chiu, and K.-C. Liu (2016), Performance of white organic light-emitting diode for portable optical biosensor. Sensors Actuators B Chemical 222, 1058–1065.

      [8] B. Derkus (2016), Applying the miniaturization technologies for biosensor design. Biosensor and Bioelectronic 79, 901–913.

      [9] Y. Liu, Q. Liu, S. Chen, F. Cheng, H. Wang, and W. Peng (2015), Surface plasmon resonance biosensor based on smart phone platforms. Scientific Reports 5, 1–9.

      [10] S. S. Zhao, N. Bukar, J. L. Toulouse, D. Pelechacz, R. Robitaille, J. N. Pelletier, and J. F. Masson (2015), Miniature multi-channel SPR instrument for methotrexate monitoring in clinical samples. Biosensors and Bioelectronic 64, 664–670.

      [11] I. Arghir, F. Delport, D. Spasic, and J. Lammertyn (2015), Smart design of fiber optic surfaces for improved plasmonic biosensing. New Biotechnology 32, 5, 473–484.

      [12] R. Chen, M. Wang, S. Wang, H. Liang, X. Hu, X. Sun, J. Zhu, L. Ma, M. Jiang, J. Hu, and J. Li (2015), A low cost surface plasmon resonance biosensor using a laser line generator. Optics Communications 349, 83–88.

      [13] H. Li, L. Zhang, H. Cai, X. Chen, J. Sun, Y. Chao, and D. Cui (2013), Portable-surface plasmon resonance biosensor immunoassays for the human serum albumin detection. Key Engineering Materials 565, 408–411.

      [14] H. Šípová, M. Piliarik, M. Vala, K. Chadt, P. Adam, M. Bocková, K. Hegnerová, and J. Homola (2011), Portable surface plasmon resonance biosensor for detection of nucleic acids. Procedia Engineering 25,148–151.

      [15] B. N. Feltis, B. A. Sexton, F. L. Glenn, M. J. Best, M. Wilkins, and T. J. Davis (2008), A hand-held surface plasmon resonance biosensor for the detection of ricin and other biological agents. Biosensors and Bioelectronic 23, 1131–1136.

      [16] K. Retra, H. Irth, and J. E. Van Muijlwijk-Koezen (2010), Surface Plasmon Resonance biosensor analysis as a useful tool in FBDD. Drug Discovery Today: Technologies 7,181–187.

      [17] T. A. Manolio (2008), Biorepositories—at the bleeding edge. International Journal of Epidemiology 37, 231–233.

      [18] P. Elliott, on behalf of UK Biobank, T. C. Peakman, and on behalf of UK Biobank (2008), The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine. International Journal of Epidemiology 37, 2, 234–244.

      [19] T. C. Peakman and P. Elliott (2008), The UK Biobank sample handling and storage validation studies. International Journal of Epidemioogy 37, i2–i6.

      [20] P. M. Baird and R. J. Frome (2005), Large-Scale Repository Design. Cell Preservation Technology 3, 256–266.

      [21] W. Mohd, A. Wan, D. Kamarun, M. Kamil, A. Rahman, and M. S. Kamarudin (2015), Modular Surface Plasmon Resonance ( SPR ) Biosensor Based on Wavelength Modulation. Advance Materials Research 1107, 699–705.

      [22] W. M. A. Wan Ahamad, D. Kamarun, and M. K. Abd Rahman (2016), Performance of newly-fabricated modular surface plasmon resonance (mSPR) sensor. Materials Science Forum 857, 459–464.

      [23] B. Grunwald, and G. Holst (2004), Fibre optic refractive index microsensor based on white-light SPR excitation. Sensors and Actuators A: Physical 113, 174–180.

      [24] A. Shrivastava and V. Gupta (2011), Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles Young Science 2, 21.

      [25] H. SadAbadi, S. Badilescu, M. Packirisamy, and R. Wüthrich (2013), Integration of gold nanoparticles in PDMS microfluidics for lab-on-a-chip plasmonic biosensing of growth hormones. Biosensors and Bioelectronic 44, 77–84.

      [26] B. Liu, Y. Pang, R. Bouhenni, E. Duah, S. Paruchuri, and L. McDonald (2015), A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor. Chemical Communications 51, 11060–11063.

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  • How to Cite

    Mohd Azwady Wan Ahamad, W., Kamarun, D., & Kamil Abd Rahman, M. (2018). BSA /anti BSA Interaction Study using a Novel Modular Surface Plasmon Resonance (m-SPR) Device. International Journal of Engineering & Technology, 7(4.18), 99-102. https://doi.org/10.14419/ijet.v7i4.18.21830