Thermodynamic Studies of Bovine Serum Albumin (BSA) Adsorption on Nylon Membrane
About this article
Abstract
The aim of this study was to investigate the nylon membrane’s characteristics and thermodynamic aspects for adsorption of Bovine Serum Albumin (BSA) as a model protein on nylon membrane. The morphology characteristics analyzed by FESEM and wetting and weighting technique showed the nylon membrane possessed micro-pore size (0.159±0.039 µm) with high porosity (74.2820±0.0411 %) respectively. Thermodynamic studies indicated that the adsorption reaction was endothermic with positive value of standard enthalpy (∆rHθ = 107.7 kJ/mole) and dominated by chemical adsorption. The results were supported with the analysis of functional groups of the nylon membrane with FTIR-ATR. Bands corresponding to the NH bonds stretching were generally detected in the 3500-3100 cm-1 range. The existence of C=O stretching vibration of carbonyl group was further confirmed with the observation of peak at 1632. 42 cm-1. The water contact angle analysis showed the hydrophilicity nature of nylon membrane with contact angle of 55.6°. These findings are expected to be used in the modification of nylon membrane for an optimum adsorption of protein. Â
Â
Keywords
- Nylon membrane
- BSA
- morphology
- thermodynamic.
References
[1] J. Marchand-Brynaert, 2006. Polymer membranes. In: Hubbard, A.T. (ed) Encyclopedia of Surface and Colloid Science. Boca Raton: Taylor & Francis.
[2] C. Charcosset “Membrane processes in biotechnology: An overview†Biotechnology Advances Volume 24, Pages 482–492, 2006.
[3] V. Hoseinpoura, A. Ghaeeb, V. Vatanpourc, N. Ghaemia “Surface modification of PES membrane via aminolysis and immobilization of carboxymethylcellulose and sulphated carboxymethylcellulose for hemodialysis†Carbohydrate Polymers Volume 188, Pages 37–47, February 2018.
[4] D. Huang, L. Niu, J. Li, J. Du, Y. Wei, Y. Hu, X. Lian, W. Chen, K. Wang “Reinforced chitosan membranes by microspheres for guided bone regeneration†Journal of Mechanical Behavior and Biomedical Materials Volume 81, Pages 195–201, March 2018.
[5] P. Afshari, N. Abolfathi “A novel method for blood-typing using nitrocellulose†Biomedical Chromatography Volume 31, Issue 7, January 2017.
[6] A.L. Ahmad, N. Ideris, B.S. Ooi, S.C. Low, A. Ismail “Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay†Polish Journal of Chemical Technology Volume 18, Issue 2, Pages 97–103, 2016.
[7] T. Tominaga “Rapid detection of Klebsiella pneumoniae, Klebsiella oxytoca, Raoultella ornithinolytica and other related bacteria in food by lateral-flow test strip immunoassays†Journal of Microbiological Methods Volume 147, Pages 43–49, March 2018.
[8] D. Chen, C. Wang, W. Chen, Y. Chen, J.X.J. Zhang “PVDF-Nafion nanomembranes coated microneedles for in vivo transcutaneous implantable glucose sensing†Biosensors and Bioelectronics Volume 74, Pages 1047–1052, July 2015.
[9] M. Mason, E. Longo, M. Scampicchio “Monitoring of glucose in beer brewing by a carbon nanotubes based nylon nanofibrous biosensor†Journal of Nanomaterials Volume 2016, May 2016.
[10] K. Nielsen, W.L. Yu, L. Kelly, J. Williams, A. Dajer, E. Gutierrez, G. Ramirez Cruz, T. Renteria, R. Bermudez, J. Algire “Validation and field assessment of a rapid lateral flow assay for detection of bovine antibody to Anaplasma marginale†Journal of Immunoassay and Immunochemistry Volume 30, Issue 3, Pages 313–321, July 2009.
[11] F. Qi, Y. Cao, M. Wang, F. Rong, Q. Xu “Nylon 6 electrospun nanofibers mat as effective sorbent for the removal of estrogens: kinetic and thermodynamic studies†Nanoscale Research Letters Volume 9, Pages 353–363, 2014.
[12] R.W. Baker, Membrane Technology, in Encyclopedia of Polymer Science and Technology, H.F. Mark, Editor 2003, A John Wiley & Sons Publication: New Jersey.p. 184-249.
[13] M. Alkan, O. Demirbas, S. Celikcapa, M. Dogan “Sorption of acid red 57 from aqueous solution onto sepiolite†Journal of Hazardous Materials B116, Pages 135–145, 2004.
[14] J. Han, W. Qiu, J. Hu, W. Gao “Chemisorption of estrone in nylon microfiltration membranes: Adsorption mechanism and potential use for estrone removal from water†Water Research Volume 46, Pages 873–881, December 2011.
[15] M. J. F. Jasni, M. Arulkumar, P. Sathishkumar, A. R. M. Yusoff, N. A. Buang, F. L. Gu “ Electrospun nylon 6,6 membrane as a reusable nano-adsorbent for bisphenol A removal: Adsorption performance and mechanism†Journal of Colloid and Interface Science Volume 508, Pages 591–602, August 2017.
[16] Adamson, A.W. (1990). Physical Chemistry of Surfaces, New York, John Wiley & Sons, Ltd.
[17] S. C. Low, R. Shaimi, Y. Thandaithabany, J. K. Lim, A. L. Ahmad, A. Ismail “Electrophoretic interactions between nitrocellulose membranes and proteins: Biointerface analysis and protein adhesion properties†Colloids and Surfaces B: Biointerfaces Volume 110, Pages 248–253, May 2013.
[18] Y. Zhou, Z. Wang, Q. Zhang, X. Xi, J. Zhang, W. Yang “Equilibrium and thermodynamic studies on adsorption of BSA using PVDF microfiltration membrane†Desalination Volume 307, Pages 61–67, September 2012.