Enhancement of Biosugar Production Via Enzymatic Yydrolysis of Sodium Hydroxide Pretreated Oil Palm Bagasse: A Statistical Perspective

  • Authors

    • Nurul Aina Fauzi
    • Shuhaida Harun
    • Jamaliah Md Jahim
    • Jian Ping Tan
    https://doi.org/10.14419/ijet.v8i1.2.24908

    Received date: December 28, 2018

    Accepted date: December 28, 2018

    Published date: January 30, 2019

  • ANOVA, commercial enzyme, glucan conversion, oil palm frond, response surface methodology

  • Abstract

    Oil palm frond bagasse (OPFB) is the major agriculture waste from the production of palm oil with 0.66g/g of total carbohydrate to serve
    as potential carbon source for downstream processing in producing succinic acid. This study focused in determining the optimum process
    variable conditions for the 1% glucan loading for enzymatic hydrolysis process with commercial blending cellulase enzyme mixture of
    Cellic CTec 2 and hemicellulase enzyme of Cellic HTec 2. Using statistical approach of Response Surface Methodology, three independent
    process variables; specific enzyme activity (5 - 80 FPU/g glucan), hydrolysis temperature (30 - 70 °C), and agitation speed (100 - 180
    rpm) were investigated at five different levels (-α,-1, 0, +1, +α). The regression models indicated that R2 for glucan conversion at 1% GL
    enzymatic hydrolysis was 97.2% showing the experimental variations were well-defined by the models. For the lack of fit test, the pvalues
    > 0.05 proves that the model was significant to the prediction model. While both specific enzyme activity and hydrolysis temperature
    were statistically significant, there was no interaction observed between these variables. Although experimental runs reported the
    maximum glucan conversion of 94% was achieved in the 1% GL hydrolysis with 83.75 FPU/g glucan and 50C after 96 hours of saccharification
    process, through validation process, the optimum conditions were determined at 30 FPU/g glucan, 45oC and 100 rpm respectively
    where these saccharification conditions achieved 90% glucan conversion within 72 hours

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  • How to Cite

    Aina Fauzi, N., Harun, S., Md Jahim, J., & Ping Tan, J. (2019). Enhancement of Biosugar Production Via Enzymatic Yydrolysis of Sodium Hydroxide Pretreated Oil Palm Bagasse: A Statistical Perspective. International Journal of Engineering and Technology, 8(1.2), 235-245. https://doi.org/10.14419/ijet.v8i1.2.24908