Organoids: life in three dimensions- a review

  • Authors

    • Akhila CNV G. Pulla Reddy Dental College and Hospital
    • Ravi Prakash A G. Pulla Reddy Dental College and Hospital
    • Rajini Kanth M G. Pulla Reddy Dental College and Hospital
    • Sreenath G G. Pulla Reddy Dental College and Hospital
    • Sowmya K G. Pulla Reddy Dental College and Hospital
    • Radhika B G. Pulla Reddy Dental College and Hospital
    2019-05-05
    https://doi.org/10.14419/ijdr.v7i1.25173
  • Cell Culture in Dentistry, Organotyping, Organoids, Organoid Culture in Dentistry, Three Dimensional Cell Cultures.

  • Most of the diseases in humans are as a result of complex interactions occurring at cellular and molecular level. Research today has been focused in an attempt to reveal precisely the cellular evolution into pathogenesis. There are vast array of research fields, which include molecular biology, imaging techniques, etc. One of such field recently advancing worldwide is “Organotypingâ€. It is the successor of two dimensional cell cultures. Miniature organs and disease models can be produced from cells having the ability to proliferate and differentiate, by adopting definite protocols. Organoids are the potential tools to probe human biology and diseases; thereby they may change the approach to study diseases and provide treatment, in a more beneficiary way to the patient. Also organoids are used in vaccine production, cancer research, microbiology, tissue regeneration, drug testing, etc. Clinical trials are more devastating and may cost life of patients included in study. As such, organoids can be included in the protocols of clinical trials, through which the outcome of the study can be estimated. They open the doors for newer research methods and innovations, which are in peak requirement of present day scenario where new diseases are emerging and the diseases already existing are not yet cured.

     

     

     

  • References

    1. [1] Jing Nie, Eri Hashino (2017) Organoid technologies meet genome engineering. EMBO Reports 18: 367–376. https://doi.org/10.15252/embr.201643732.

      [2] Eliah R. Shamir and Andrew J. Ewald (2014) Three-dimensional organotypic culture: experimental models of mammalian biology and disease. Nature Reviews Molecular Cell Biology 15: 647–664. https://doi.org/10.1038/nrm3873.

      [3] Aliya et al., (2016) Organoids as an in vitro model of human development and disease. Nature Cell Biology 18: 246-254. https://doi.org/10.1038/ncb3312.

      [4] Anna and Helena (2006) Development of a highly reproducible three-dimensional organotypic model of the oral mucosa. Nature Protocols 1: 2012–2018. https://doi.org/10.1038/nprot.2006.323.

      [5] Ninouk Akkerman and Libert H.K. Deï¬ze (2017) Dawn of the organoid era. Bioessays: 1600244. https://doi.org/10.1002/bies.201600244.

      [6] Eriksson, Day, Fedele and Salih (2016) The regulation of bone turnover in ameloblastoma using an organotypic in vitro co-culture model. Journal of Tissue Engineering 7: 1 –8. https://doi.org/10.1177/2041731416669629.

      [7] Senthil K. Muthuswamy (2017) Bringing together the organoid field: from early beginnings to the road ahead. Development 144: 963-967. https://doi.org/10.1242/dev.144444.

      [8] Takebe et al., (2017) Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells. Cell Reports 21: 2661–2670. https://doi.org/10.1016/j.celrep.2017.11.005.

      [9] Hisha et al., (2016) Lingual Epithelial Stem Cells and Organoid Culture of Them. International Journal of Molecular Sciences 17: 168. https://doi.org/10.3390/ijms17020168.

      [10] Aihara et al., (2015) Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid. Scientific Reports 5: 17185. https://doi.org/10.1038/srep17185.

      [11] Ren W et al., (2017) Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation. Scientific Reports 7: 4004. https://doi.org/10.1038/s41598-017-04099-5.

      [12] Nanduri et al., (2014) Purification and Ex Vivo Expansion of Fully Functional Salivary Gland Stem Cells. Stem cell reports 3: 957-964. https://doi.org/10.1016/j.stemcr.2014.09.015.

      [13] Papapetrou (2016) Patient-derived induced pluripotent stem cells in cancer research and precision oncology. Nature medicine 22: 1392-1401. https://doi.org/10.1038/nm.4238.

      [14] Angela Saini (2016) Cystic Fibrosis Patients Benefit from Mini Guts. Stem Cell 6: 425-427. https://doi.org/10.1016/j.stem.2016.09.001.

      [15] Ma L, et al., (2017) Organoid culture of human prostate cancer cell lines LNCaP and C4-2B. American Journal of Clinical and Experimental Urology 5: 25-33.

      [16] Baker et al., (2016) Modeling pancreatic cancer with organoids. Trends in cancer 2:176-190. https://doi.org/10.1016/j.trecan.2016.03.004.

      [17] Gao et al., (2017) 3D Spheroid/Organoid Models of Lung Cancer to Study Lung Cancer Pathogenesis and Testing of New Therapeutics. Journal of Thoracic Oncology 12: S1544. https://doi.org/10.1016/j.jtho.2017.06.053.

      [18] Lovisa et al., (2015) Targeting Toll-like receptor 2 inhibits growth of head and neck squamous cell carcinoma. Oncotarget 6: 9897-9907. https://doi.org/10.18632/oncotarget.3393.

      [19] Edmondson et al., (2014) Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors: Assay and Drug Development Technologies 12: 207-218. https://doi.org/10.1089/adt.2014.573.

      [20] Skardal et al., (2017) Multi-tissue interactions in an integrated three-tissue organ-on-achip platform. Scientific reports 7: 8837. DOI: https://doi.org/10.1038/s41598-017-08879-x.

      [21] Driehuis and Clevers (2017) CRISPR/Cas 9 genome editing and its applications in organoids. American Journal of Physiology 312: 257–265 https://doi.org/10.1152/ajpgi.00410.2016.

      [22] Schmidt et al., (2016) Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer. Oncology Reports, 35, 2431-2440. https://doi.org/10.3892/or.2016.4581.

      [23] Dongari et al., (2006) Development of a novel three-dimensional in vitro model of oral Candida infection. Microbial pathogenesis 40: 271-8. https://doi.org/10.1016/j.micpath.2006.02.004.

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  • How to Cite

    CNV, A., Prakash A, R., Kanth M, R., G, S., K, S., & B, R. (2019). Organoids: life in three dimensions- a review. International Journal of Dental Research, 7(1), 5-7. https://doi.org/10.14419/ijdr.v7i1.25173