Evaluation of the efficacy of a locally formulated antiseptic, for the control of disease-causing microorganisms
Abstract
An antiseptic (sample A) was locally formulated. Its efficacy on the following micro-organisms; E.Coli, staphylococcus, streptococcus and bacillus species was evaluated and compared with that of Dettol and Septol. Sample A was established to be a cheap and effective antiseptic, which is capable of competing favourably with those in the Nigerian market. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) indicated that Sample A had a similar efficacy with Dettol. The P-value of the MIC results for samples A, Dettol and Septol were 0.004, 0.268 and 0.080 respectively, while the P-value of the MBC results were 0.450, 0.077 and 0.043 respectively. The standard errors of the mean were 0.079, 0.072 and 0.086 for sample A, Dettol and Septol respectively.
About this article
Keywords
- Antiseptic
- Chloroxylenol
- Micro-Organism
- Bactericidal and Inhibitory.
References
[1] Ngan V (2005) What is an antiseptic? Available at: https://www.dermnetnz.org/topics/antiseptic/. Accessed April 25, 2020.
[2] Khan HA, Baig FK & Mehboob R (2017) Nosocomial infections: Epidemiology, prevention, control and surveillance Asian Pacific Journal of Tropical Biomedicine 5, 478-482. https://doi.org/10.1016/j.apjtb.2017.01.019.
[3] Larson EL & Morton HE (1991) Alcohols. In: Block, S.S., Ed., Disinfection, Sterilization, and Preservation, 4th Edition, Lea and Febiger, Philadelphia, PA, 191-203. Available at: https://www.scirp.org. Accessed April 25, 2020.
[4] Russell AD (1996) Activity of biocides against mycobacteria J. Appl. Bacteriol. Symp. Suppl, 81, 87-101. https://doi.org/10.1111/j.1365-2672.1996.tb04602.x.
[5] Chapman JS (2003) Disinfectant resistance mechanisms, cross-resistance, and co-resistance International Biodeterioration & Biodegradation 51, 271-276. https://doi.org/10.1016/S0964-8305(03)00044-1.
[6] Giuliana G, Pizzo G, Milici M, Musotto GC & Giangreco R (1997) In vitro antifungal properties of mouth rinses containing antimicrobial agents Journal Periodontol 68, 729-733. https://doi.org/10.1902/jop.1997.68.8.729.
[7] Lamikanra A (2010) Essential Microbiology for Students and Practitioner of Pharmacy, Medicine and Microbiology 2nd Edn. Lagos: Amkra books; 406.
[8] Gorman SP (2007) Chemical Disinfectants, Antiseptics and Preservatives In book: Hugo and Russell's: Pharmaceutical Microbiology, Seventh Edition. Available at: https:// www.wiley.com/go/denyer/microbiology. Accessed April 27, 2020.
[9] Nanzip BN (2020) Formula for Homemade Antiseptic or Disinfectant. Available at: https:// jotsrcoll.com. Accessed April 1, 2020.
[10] Sagar (2017) Nutrient Agar and Nutrient Broth: Composition, Preparation and Differences. Available at: https:// www. LabMal.com. Accessed April 10, 2020.
[11] Maris P (1995) Modes of action of disinfectants Review Scientific Technique 14, 47-55. https://doi.org/10.20506/rst.14.1.829.
[12] Tripathi KD (2013) Essentials of Medical Pharmacology (7th ed.). New Delhi, India: Jaypee Brothers Medical Publishers ISBN 9789350259375. https://doi.org/10.5005/jp/books/12256.
[13] Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Assay. Available at: http://www emerypharma.com. Accessed January 18, 2020.
[14] Zone of Inhibition Test for Antimicrobial Activity. Available at: http://www.microchemlab.com. Accesed June 6, 2020.
[15] French GL (2006) Bactericidal agents in the treatment of MRSA infections-the potential role of daptomycin. Journal of Antimicrobial Chemotherapy, 56 1107-1117. https://doi.org/10.1093/jac/dkl393.
[16] McDonnell G & Russell AD (2001) Antiseptics and Disinfectants: Activity, Action, and Resistance Clinical Microbiology Reviews 14, 227. PMCID: PMC88971. https://doi.org/10.1128/CMR.14.1.227-227.2001.