In-Vitro α-Glucosidase Inhibitory Activities of Muntingia Calabura Linn.

  • Authors

    • N I.I. Nor Azman
    • N Hashim
    • R Ahmad
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27525
  • Muntingia calabura, antidiabetic, postprandial hyperglycemia, α-glucosidase

  • Muntingia calabura Linn. also known locally as “ceri kampung†is a plant of the family Elaeocarpaceae. The plant has been reported to possess several medicinal properties including reducing high blood pressure, lowering cholesterol level and controlling Type 2 diabetes. Type 2 diabetes is usually related to postprandial hyperglycemia, which is related to the rise of blood sugar level after a meal. This condition can be controlled by α-glucosidase inhibitors which inhibit the enzyme from catalyzing the liberation of glucose from carbohydrates in the digestive tract. Despite many biological studies reported for the plant, its antidiabetic potential has not been widely explored. Thus the aim of this study was to find potential α-glucosidase inhibitors from 16 extracts of M. calabura as a therapeutic approach in decreasing postprandial hyperglycemia. The hexane (Hx), ethyl acetate (Ea), 75% ethanol (Et) and aqueous (Aq) extracts of four parts (fruit, leaf, stem and root) of M. calabura (collected from Bangi, Selangor) were screened for their a-glucosidase inhibitory activities at 50.00, 25.00, 12.50, 6.25, 3.13, 1.56 and 0.78 ppm prepared via two-fold serial dilution against the positive control, acarbose. The aqueous leaf (AqL) and root extracts (AqR) exhibited very strong activities with IC50 values of 0.15 and 0.41 µg/ml  while the other extracts showed strong to moderately strong activities with IC50 values ranging from 1.83-11.66  µg/ml against acarbose (4.3 µg/ml).

     

  • References

    1. [1] M. I. M. Yusof, M. Z. Salleh, T. L. Kek, N. Ahmat, N. F. N. Azmin, and Z. A. Zakaria, “Activity-guided isolation of bioactive constituents with antinociceptive activity from Muntingia calabura L . leaves using the formalin test * Address all correspondence to : Associate Professor Dr . Zainul Amiruddin Zakaria Department of Biomedical Scien,†Hindawi, vol. 2013, pp. 1–27, 2013.

      [2] Z. A. Zakaria et al., “Antinociceptive, anti-inflammatory and antipyretic effects of Muntingia calabura aqueous extract in animal models,†J. Nat. Med., vol. 61, no. 4, pp. 443–448, 2007.

      [3] N. D. Mahmood et al., Muntingia calabura: A review of its traditional uses, chemical properties, and pharmacological observations, vol. 52, no. 12. 2014.

      [4] E. S. Huang, A. Basu, M. O’Grady, and J. C. Capretta, “Projecting the future diabetes population size and related costs for the U.S.,†Diabetes Care, vol. 32, no. 12, pp. 2225–2229, 2009.

      [5] A. B. Olokoba, O. A. Obateru, and L. B. Olokoba, “Type 2 diabetes mellitus: A review of current trends,†Oman Med. J., vol. 27, no. 4, pp. 269–273, 2012.

      [6] A. Ceriello, “Postprandial Glucose Regulation and Diabetic Complications,†Arch. Intern. Med., vol. 164, no. 19, p. 2090, Oct. 2004.

      [7] A. Banerjee, B. Maji, S. Mukherjee, K. Chaudhuri, and T. Seal, “In Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis,†J. Appl. Biol. Biotechnol., vol. 5, no. 03, pp. 61–67, 2017.

      [8] R. Ahmad et al., “Antioxidant and Antidiabetic Potential of Malaysian Uncaria,†Res. J. Med. Plant, vol. 5, no. 5, pp. 587–595, May 2011.

      [9] M. Aruna Sindhe, Y. D. Bodke, and A. Chandrashekar, “Antioxidant and in vivo anti-hyperglycemic activity of muntingia calabura leaves extracts,†Der Pharm. Lett., vol. 5, no. 3, pp. 427–435, 2013.

      [10] D. Ramadas, S. Chandrappa, H. R. Kashyap, A. Biotechnology, and M. Sciences, “in Vitro Anti-Diabetic Activity of Muntingia,†vol. 4, no. 10, pp. 1526–1534, 2015.

      [11] V. K. Yadav and A. Mishra, “In vitro & in silico study of hypoglycemic potential of Pterocarpus marsupium heartwood extract,†Nat. Prod. Res., vol. 6419, no. May, pp. 1–5, 2018.

      [12] A. S. Sufian, K. Ramasamy, N. Ahmat, Z. A. Zakaria, and M. I. M. Yusof, “Isolation and identification of antibacterial and cytotoxic compounds from the leaves of Muntingia calabura L.,†J. Ethnopharmacol., vol. 146, no. 1, pp. 198–204, 2013.

      [13] J. J. Chen, R. W. Lin, C. Y. Duh, H. Y. Huang, and I. S. Chen, “Flavones and cytotoxic constituents from the stem bark of Muntingia calabura,†J. Chinese Chem. Soc., vol. 51, no. 3, pp. 665–670, 2004.

  • Downloads

  • How to Cite

    I.I. Nor Azman, N., Hashim, N., & Ahmad, R. (2019). In-Vitro α-Glucosidase Inhibitory Activities of Muntingia Calabura Linn. International Journal of Engineering & Technology, 7(4.14), 183-185. https://doi.org/10.14419/ijet.v7i4.14.27525