Comparison of Microwave and Ultrasonic Assisted Extraction of Kaempferol from Cassia Alata

  • Authors

    • Yi Ling Yeong
    • Sook Fun Pang
    • Sin Yee Chong
    • Jolius Gimbun
    2018-07-27
    https://doi.org/10.14419/ijet.v7i3.13.16330
  • Auxiliary energy, Cassia alata, gelenggang, kaempferol, microwave assisted extraction.
  • The effect of auxiliary energy to the kaempferol extraction yield from Cassia alata is presented in this paper. The effect of auxiliary energy ranging from 0.045 to 20 W/ml was studied by performing extraction using ultrasonic assisted extraction (UAE), maceration (ME) and microwave assisted extraction (MAE). A ultra-performance liquid chromatography coupled photodiode array (UPLC-PDA) was used for kaempferol identification and quantification. Matching of residence time and UV spectrum between the authentic standard and plant extract was observed to confirm the presence of kaemferol. It was found that MAE method has the highest extraction yield of kaempferol at 21.55 mg/g DW followed by UAE (18.60 mg/g DW) and ME (12.01 mg/g DW). Besides that, the extraction time of MAE was the shortest which is only 4 minutes. The optimum conditions to obtain a high kaempferol yield was achieved using 100% ethanol to extract C. alata powdered leaves with PSD ≤125µm and plant solid to solvent ratio of 1:20. The findings in this work may serve as a useful guide to obtain highest extraction yield of kaempferol from C. alata.

     

     

  • References

    1. [1] Rajagopal PL, Majetha R, Sreejith KR, Kiron SS, Aneeshia S & Premaletha K (2014), Phytopharmacological review on Cassia alata Linn. Journal of International Academic Research Multidisciplinary 2, 280-307.

      [2] Fernand VE, Dinh DT, Washington SJ, Fakayode SO, Losso JN, Ravenswaay ROV & Warner IM (2008), Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography. Talanta 74, 896-902.

      [3] Okwu DE & Nnamdi FU (2011), Cannabinoid dronabinol alkaloid with antimicrobial activity from Cassia alata Linn. Der Chemica Sinica 2, 247-254.

      [4] Escobar CAL, Capote FP & Castro MDL (2015), Comparative study of the effect of auxiliary energies on the extraction of Citrus fruit components. Talanta 144, 522-528.

      [5] Pang SF, Lau MZ, Yusoff MM & Gimbun J (2017), Microwave-irradiation induced fast simultaneous extraction of methoxylated and hydroxylated phenolic compounds from Orthosiphon stamineus leaves. Material Science Forum, 155-158.

      [6] Nguang SL, Yeong YL, Pang SF & Gimbun J (2017), Ultrasonic assisted extraction on phenolic and flavonoid content from Phyllanthus niruri plant. India Journal of Science Technology 10.

      [7] Sule WF, Okonko IO, Omo-Ogun S, Nwamze JC, Ojezele MO, Ojezele OJ, Alli JA, Soyemi ET & Olaonipekun TO (2011), Phytochemical properties and in-vitro antifungal activity of Senna alata Linn. crude stem bark extract. Journal of Medicinal Plants Research 5, 176-183.

      [8] Gritsanapan W & Mangmeesri P (2009), Standardized Senna alata leaf extract. Journal of Health Research 23, 59-64.

      [9] Aminuddin MF, Basri AM, Taha H, Abidin AM & Ahmad N (2016), Antimicrobial activities of soaps containing Senna alata leaf extract. Scientia Bruneiana Special Issue.

      [10] Oladeji O, Adelowo F, Odelade K, Aremu S & Adisa M (2016), Senna alata: phytochemical components, characterization and medical benefits. CIBTech. Journal of Pharmaceutical Sciences 5, 40-51.

      [11] Pandey D & Gupta AK (2015), New Dimensions in Microbiology, Lenin Media Private Limited, Delhi, India, 53-67.

      [12] Varghese GK, Bose LV & Habtemariam S (2012), Antidiabetic components of Cassia alata leaves: identification through α–glucosidase inhibition studies. Pharmarceutical Biology 3, 345-349.

      [13] Turkmen N, Sari F & Velioglu YS (2006), Effect of extraction solvents on concentration and antioxidant activity of black and black mate polyphenols determined by 9ferrous tartrate and Folin-Ciocalteu methods. Food Chemistry 99, 838-841.

      [14] Bae H, Jayaprakasha GK, Jifon J & Patil BS (2011), Extraction efficiency and validation of an HPLC method for flavonoid analysis in peppers. Food Chemistry 130, 751-758.

      [15] Silva EM, Rogez H & Larondelle Y (2007), Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology. Separation and Purification Technology 55, 381–387.

      [16] Ćujić N, Šavikin K, Jankovic T, Pljevljakušic D, Zdunić G & Ibrić S (2016), Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry 194, 135–142.

      [17] Yang Y & Zhang F (2008), Ultrasound-assisted extraction of rutin and quercetin from Euonymus alatus (Thunb). Sieb. Ultrasonics Sonochemistry 64, 3-19.

      [18] Tan PW, Tan CP & Ho CW (2011), Antioxidant properties: effect of solid-to-solvent ratio on antioxidant compounds and capacities of pegaga (Centella asiatica). International Food Research Journal 18, 553-558.

      [19] Li H, Pordesimo L & Weiss J (2004), High intensity ultrasound-assisted extraction of oil from soybeans. Food Research International 37, 731-738.

      [20] Herrera MC & Castro MDL (2005), Ultrasound-assisted extraction of phenolic compounds from strawberries prior to liquid chromatographic separation and photodiode array ultraviolet detection. Journal of Chromatography 1100, 1-7.

      [21] Tuulmets A & Salmar S (2001), Effect of ultrasound on esters hydrolysis in aqueous ethanol. Ultrasonics Sonochemistry 8, 209-212.

      [22] Boonkird S, Phisalaphong C & Phisalaphong M (2008), Ultrasound-assisted extraction of capsaicinoids from Capsicum frutescens on a lab and pilot-plant scale. Ultrasonics Sonochemistry 15, 1075-1079.

      [23] Vajić UJ, Grujić-Milanović J, Živković J, Šavikin K, Gođevac D, Miloradović Z, Bugarski B & Mihailović-Sta-nojević N (2015), Optimization of extraction of stinging nettle leaf of phenolic compounds using response surface methodology. Industrial Crops and Products 4, 912–917.

      [24] Uma DB, Ho CW & Aida WMW (2010), Optimization of Extraction Parameters of Total Phenolic Compounds from Hena (Lawsonia intermis) Leaves. Sains Malaysiana 39, 19–128.

      [25] Chew KK, Khoo MZ, Ng SY, Thoo YY, Aida WMW & Ho CW (2011), Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineous extracts. International Food Research Journal 18, 27-35.

      [26] Lovrić V, Putnik P, KovaÄević DB, Jukić M & Dragović-uzelac V, Effect of Microwave-Assisted Extraction on the Phenolic Compounds and Antioxidant Capacity of Blackthorn Flowers. Food Technology and Biotechnology 55, 243–250.

      [27] Li Y, Li S, Lin SJ, Zhang JJ, Zhao CN & Li HB (2017), Microwave-assisted extraction of natural antioxidants from the exotic gordonia axillaris fruit : optimization and identification of. phenolic compounds. Molecules 22, 1481.

      [28] Yeong YL, Pang SF, & Gimbun J, (2017), The effect of auxiliary energy on rhein, kaempferol and astragalin extraction from Cassia alata. MATEC Web of Conferences 111, 02009.

  • Downloads

  • How to Cite

    Ling Yeong, Y., Fun Pang, S., Yee Chong, S., & Gimbun, J. (2018). Comparison of Microwave and Ultrasonic Assisted Extraction of Kaempferol from Cassia Alata. International Journal of Engineering & Technology, 7(3.13), 84-89. https://doi.org/10.14419/ijet.v7i3.13.16330