Impact of different fertilizers on some phytochemical constituents and biological activities of Beta vulgaris L. leaves

  • Authors

    • Ghada farouk researcher in the national organization for drug control and research
    • Horia Mostafa researcher in the national organiztion for drug control and research
    2017-01-30
    https://doi.org/10.14419/ijbas.v6i1.7228
  • Antimicrobial, Antioxidant, Cytotoxic, Fertilizers, Red Beet Leaves.
  • Red beet leaves are used in some countries in salads, while others consider them as leftover. Our goal is to conduct field experiment, for two seasons, to evaluate the effect of various combinations of (organic, chemical and bio-/yeast) fertilizers and different harvest dates on some leaves growth parameters, biocostituents and biological activities. The applied fertilizers significantly increased most of the growth parameters compared to control and highest values were produced by 100%chemical fertilizer (2), followed by treatments of 75%biofertilizer (4) and 75%yeast (8). Highest chlorophyll (a&b) and carotenoids contents were recorded by treatments 2, 4 and 8, particularly those of the second stage. Most of the studied fertilizers significantly increased betanin and polyphenolic contents compared to control and highest values were attained by treatments 4 and 8. Maximum values of betanin and polyphenols were reached in the first and third stages, respectively. Additionally, highest antioxidant activity was afforded by leaves of treatments 4 and 8, whereas leaves of treatment 4 provided a broader antimicrobial activity than control. Moreover, the highest cytotoxic activity was exerted by leaves of treatment 4. Finally, it is recommended to harvest beet leaves at the appropriate stage to obtain high growth values and reasonable percentages of bioactive metabolites.

  • References

    1. [1] Umesh Rudrappa. Beet greens nutrition facts. http://www.nutrition-and-you.com/beet-greens.html.

      [2] P.B.F. Biondo, J.S. Boeing, É.O. Barizão, et al., Evaluation of beetroot (Beta vulgaris L.) leaves during its developmental stages: a chemical composition study, Food Science and Technology (Campinas) 34, 1 (2014) 94-101. https://doi.org/10.1590/S0101-20612014005000007.

      [3] N.K. Jain, A.K. Singhai, Protective role of Beta vulgaris L. leaves extract and fractions on ethanol-mediated hepatic toxicity, Acta Poloniae Pharmaceutica-Drug Research 69, 5 (2012) 945-950.

      [4] M. H. Ibrahim, H. Z. E. Jaafar, E. Karimi, A. Ghasemzadeh,Impact of organic and inorganic fertilizers application on the phytochemical and antioxidant activity of Kacip Fatimah (Labisia pumila Benth), Molecules 18 (2013) 10973-10988.https://doi.org/10.3390/molecules180910973.

      [5] F.P. Weibel, R. Bickel, S. Leuthold, T. Alfoldi, Are organically grown apples tastier and healthier? A comparative field study using conventional and alternative methods to measure fruit quality, Acta Horticulturae 517 (2000) 417–426.https://doi.org/10.17660/ActaHortic.2000.517.53.

      [6] S.H. Hakkinen, A.R. Torronen, Content of flavonols and selected phenolic acids in strawberries and Vaccinium species: Influence of cultivar, cultivation site and technique, Food Research International 33 (2000) 517–524.https://doi.org/10.1016/S0963-9969(00)00086-7.

      [7] D.K. Asami, Y.J. Hong, D.M. Barrett, A.E. Mitchell, Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn using conventional, organic, and sustainable agricultural practices, Journal of Agricultural and Food Chemistry 51 (2003) 1237–1241.https://doi.org/10.1021/jf020635c.

      [8] G. Farouk, H. Sharawy, Effect of different fertilizers on growth and bioconstituents of red beet bulbs during three maturity stages, Advances in Environmental Biology 10, 1 (2016) 33-41.

      [9] W.P. Inskeep, P.R. Bloom, Extinction coefficients of chlorophyll a & b in NN-dimethylformade and 80% acetone, Plant Physiology 77 (1985) 483-485.https://doi.org/10.1104/pp.77.2.483.

      [10] M. Piattelli, L. Minale, Pigments of centrospermae I. Betacyanins from Phyllocactus hybridus Hort. and Opuntia ficus-indica Mill., Phytochemistry 3 (1964) 307-311.https://doi.org/10.1016/S0031-9422(00)88056-5.

      [11] J. Lachman, D. Proněk, A. Hejtmánková, V. Pivec, K. Faitová, Total polyphenol and main flavonoid antioxidants in different onion (Allium cepa L.) varieties, Scientia Horticulturae 30 (2003) 142-147.

      [12] W. Brand-Williams, M.E. Cuvelier, C. Berset, Use of a free radical method to evaluate antioxidant activity, LWT - Food Science and Technology 28 (1995) 25-30.https://doi.org/10.1016/S0023-6438(95)80008-5.

      [13] C. Perez, M. Paul, P. Bazerque, Antibiotic assay by agar-well diffusion method, Acta biologiae et medicinae experimentalis 15 (1990) 113-115.

      [14] J.M. Andrews, Determination of minimum inhibitory concentrations, Journal of Antimicrobial chemotherapy 48 (2001) 5-16.https://doi.org/10.1093/jac/48.suppl_1.5.

      [15] P. Skehan, R. Storeng, D. Scudiero, et al., New colorimetric cytotoxicity assay for anticancer-Drug screening, Journal of the National Cancer Institute 82 (1990) 1107-1112.https://doi.org/10.1093/jnci/82.13.1107.

      [16] A.S. El-Khawaga, Selecting the optimum amount of organic, mineral and biofertilizers responsible for maximizing the productivity of Balady guava trees, Egyptian journal of applied science 22, 5B (2007) 14-26.

      [17] M.M. Farahat, E.M.F. El- Quesni, M.A. El-Khateeb, A.S. El-Leithy, K.I. Hashish, Impact of combined chemical and biofertilizers on vegetative growth and chemical composition of Paulownia kawakamii seedlings, Middle East Journal of Agriculture Research 3, 4 (2014) 852-858.

      [18] P. Nakayan, F.T. Shen, M.H. Hung, C.C. Young, Effectiveness of Pichia sp. CC1 in decreasing chemical fertilization requirements of garden lettuce in pot experiments. Asian Journal of Food and Agro-Industry, special (2009) S66-S68.

      [19] M.A.W. Asal,The combined Effect of mineral organic and biofertilizers on the productivity and quality of some wheat cultivars, Ph.D. Thesis, Fac. Agric. Alex. Univ., Egypt, 2010.

      [20] R. Agamy, M. Hashem, S. Alamri, Effect of soil amendment with yeasts as bio-fertilizers on the growth and productivity of sugar beet, African Journal of Agricultural Research 8, 1 (2013) 46-56.

      [21] S. I. Shedeed, S.A.A EL-Sayed, D.M. Abo Bash, Effectiveness of bio-fertilizers with organic matter on the growth, yield and nutrient content of Onion (Allium cepa L.) plants, European International Journal of Science and Technology 3, 9 (2014) 115-122.

      [22] H.A.A. Taie, R. El-Mergawi, S. Radwan, Isoflavonoids, flavonoids, phenolic acids profiles and antioxidant activity of soybean seeds as affected by organic and bioorganic fertilization, American-Eurasian Journal of Agricultural & Environmental Sciences 4, 2 (2008) 207-213.

      [23] F.M.A. Matter, S.A.A. El Sayed, Influence of Mixed NPK Fertilizers with Foliar Spray of Active Dry Yeast on Caraway under Newly Reclaimed Soil Conditions, Middle East Journal of Applied Sciences 5, 2 (2015) 423-430.

      [24] C.Y. Hsu, P.Y. Chao, S.P. Hu, C.M. Yang, The Antioxidant and free radical scavenging activities of chlorophylls and pheophytins, Food and Nutrition Sciences 4 (2013) 1-8.https://doi.org/10.4236/fns.2013.48A001.

      [25] W. Stahl, H. Sies, Antioxidant activity of carotenoids, Molecular Aspects of Medicine 24, 6 (2003) 345-51. https://doi.org/10.1016/S0098-2997(03)00030-X.

      [26] T. Esatbeyoglu,A.E. Wagner,R. Motafakkerazad,Y. Nakajima,S. Matsugo,G. Rimbach, Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells,Food and Chemical Toxicology 73 (2014) 119–126. https://doi.org/10.1016/j.fct.2014.08.007.

      [27] K.B. Pandey, S.I. Rizvi, Plant polyphenols as dietary antioxidants in human health and disease, Oxidative Medicine and Cellular Longevity 2, 5 (2009) 270–278.https://doi.org/10.4161/oxim.2.5.9498.

      [28] A.S. Velićanski, D.D. Cvetković, S.L. Markov, J.J. Vulić, S.M. Djilas. Antibacterial activity of Beta vulgaris L. pomace extract,Acta periodica technologica 42 (2011) 263-269.

      [29] J.J. Vulic, T.N. Cebovi, V.M. Canadanovi, et al., Antiradical, antimicrobial and cytotoxic activities of commercial beetroot pomace, Food & Function 4 (2013) 713-721.https://doi.org/10.1039/c3fo30315b.

      [30] J. Parekh, V. Chanda, Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants against some Staphylococcus species, Turkish Journal of Biology 32 (2008) 63–71.

      [31] L. Nowacki, P. Vigneron, L. Rotellini, et al., Betanin-Enriched Red Beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells, Phytotherapy Research 29 (2015) 1964–1973.https://doi.org/10.1002/ptr.5491.

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    farouk, G., & Mostafa, H. (2017). Impact of different fertilizers on some phytochemical constituents and biological activities of Beta vulgaris L. leaves. International Journal of Basic and Applied Sciences, 6(1), 1-6. https://doi.org/10.14419/ijbas.v6i1.7228