Studies on the effects of cephradine and colibacellosis on immunological status of broiler chicken vaccinated with newcastle virus vaccine

  • Authors

    • Ashraf Elkomy professor of pharmacology
    • Mohamed Aboubakr
    • Elsayed Emam
    • Mohammed Kassem
    2019-07-22
    https://doi.org/10.14419/ijpt.v7i2.29020
  • Broiler chickens, Cephradine, Colibacillosis, Immunity, Vaccine.

  • The present study was carried out using 100, one-day old broiler chicks to evaluate the immunological status of broiler chicks vaccinated with Newcastle virus vaccine and infected with E coli and treated by cephradinee.At day 15th of age, broilers chicks were divided into 4 equal groups (25 chicks in each). 1st group, healthy non infected non treated broilers (control group). 2nd, 3rd and 4th groups expermintally infected with E. coli was done at 15th day of age. 2nd group infected, non treated broilers, 3rd group infected broilers and vaccinated with Newcastle disease virus vaccine, 4th group infected broilers vaccinated with Newcastle vaccine and received 20 mg/kg b.wtcephradinee in drinking water daily for 5 consecutive days. At 1st, 10th and 20th day post administration, blood samples were collected for determination cellular and humeral immune response. Infected broilers with E coli only or infected broilers and vaccinated display significant increase in leukocyte, heterophils, phagocytic activity, phagocytic index, killing percentage, nitric oxide, lysozyme activity and gamma globulin. Beside significant decrease in lymphocyte, serum total protein, albumin, total globulin, A/G ratio and HI titer coupled with insignificant decrease in esinophils, basophils and monocyte, beta globulin associated with insignificant increase in alpha globulin allover experimental period post vaccination when compared with control broilers. Vaccinated-Infected broilers that received 20 mg/kg b.wtcephradine daily for five consecutive days revealed significant increase in leukocyte, heterophils, phagocytic activity, phagocytic index, killing % and gamma globulin at 1st day post treatment coupled with insignificant increase at 10th and 20th day post treatment. In-addition to significant decrease in serum total protein, albumin, total globulin A/G ratio and HI associated with non significant decrease in esinophils, basophils, monocyte,beta globulin and non significant increase in nitric oxide, lysozyme activity and alpha globulin allover the experiment when compared with control broilers. It could be concluded that, colibacillosis in broiler chickens and cephradine induced some adverse effects on immunological status of broiler chickens. Therefore, it’s important not vaccinated broiler during colibacelosis or using cephradine in treatment.

     

     

  • References

    1. [1] Abd El hafez, S.; Manal, B. and Amer, H. (2006) Enhancement the Efficacy of Cephalosporins with E Coli Periplasmic Protein to Control E. ColiInfection with Special Reference to Pneumonic Manifestations. SCVMJ, X (1), 415-428.

      [2] Abd El-Aziz, M. (2006):Handbook of Veterinary Pharmacology, 5th Ed.

      [3] Abd El-Ghany, W. and Ismail, M. (2014) Tackling experimental colisepticae-mia in broiler chickens using phytobiotic essential oils and antibiotic alone or in combination. Iranian J Vet Res, Shiraz Uni 15(2)110-115.

      [4] Abdallah, SA. (2015) Influence of cephalexin on Immuno status of vaccinated Rabbits. MVSc. Thesis Dept. of Bird and Rabbit Diseases, Fac. Vet. Med, Zag. University.

      [5] Aboubakr, M and Elbadawy M (2017) Bioavailability, pharmacokinetics and tissue residues of cephradine (Atocef Forte®) in healthy and colisepticemic broiler chickens. International Journal of Pharmacology and Toxicology, 5 (1): 57-60 https://doi.org/10.14419/ijpt.v5i1.7428.

      [6] Ahmad, S (2009) effect of certifier sodium (excenel) on immune response of vaccinated chickens. PhD Theses submitted to Fac of Vet Med.Zag Uni.

      [7] Allam, H; Eman, S; Salah, H Rashidy, R. and Adel, E. (2014) Effect Organic Acidsand Probiotic on broiler Blood Parameters and Control of E. coli. Zag.Vet. J. 42:1.

      [8] Awad A., El-Hofy, FI; Khalid I and El-Shora HE. (2015) Effect of Synbiotic on immune response of experimentally infected broiler chickens with E. coli and salmonella. Benha Vet. Med. J. 28. 2:188-194https://doi.org/10.21608/bvmj.2015.32501.

      [9] Borowski, J.; Jakoniok, P. and Talarczyk, J. (1984): The influence of some cephalosporins as immunological response. Antibiotic (Tokoyo), 37(12):719-726.

      [10] Braga, P.; Dal Sasso, M.; Mancini, L. and Sala, M. (1999): Influence of sub–minimum inhibitory concentrations of cefodizime on phagocytosis, Intracellular killing and oxidative Bursts of human polymorpho-nuclear leukocytes. Chemotherapy. J., 45(3): 166-174.https://doi.org/10.1159/000007179.

      [11] Bredt, D. and Snyder, S. (1994): Nitric oxide, a physiological messenger molecule. Ann Rev Biochem 63: 175-195 https://doi.org/10.1146/annurev.bi.63.070194.001135.

      [12] Calnek, B.; Barnes, H.; Beard, C.; McDougald, L. and Saif Y. (1997) Diseases of Poultry. 10thed. Iowa State University Press; Ames, IA, USA.

      [13] Coleman, J. (2001) Nitric oxide in immunity and inflammation. Int. Immunopharmacol. 1: 1397-1406https://doi.org/10.1016/S1567-5769(01)00086-8.

      [14] Coles, E (1986). Veterinary Clinical Pathology. (EH Cole, editor), Fourth edition. WB Saunders, New York. Pp 43–72.

      [15] Darrell R.; Claudio L. and Patti J. (2013) Immune responses of poultry to Newcastle disease virus.Developmental & Comparative Immunology, 41(3): 47-53https://doi.org/10.1016/j.dci.2013.04.012.

      [16] Deng, R.; Xu, Y. and Yu, N. (1991) Dynamic study on antibody-forming cells and antibody-secreting cells in the initial stage post immunization with Rabbit hemorrhagic disease vaccine (in Chinese). Sci. agric. Sinica, 24 (1), 21-30.

      [17] Doumas, B.; Cartor, R.; Peers, T. and Schaffer, R. (1981): Acandidate reference method for determination of total protein in serum Clin Chem, 27, 1642.

      [18] Doxey, D (l983): Clinical pathology and diagnostic procedure 2ndEd.Baillier London.

      [19] El Sayed, M.; Allam, H.; El Nabarawy, E. and Eman, S. (2014): bioche-mical and bacteriological studies on mortality in newly born rabbits. Zag. Vet. J. 42 (3) 56-66.

      [20] EL Sayed, M.G.; Aboubakr, M and Rabea, S (2016) â€Pharmacokinetics and tissue residues of cephradine in healthy and experimentally Salmonella entretidis infected broiler chickensâ€. World Journal of Pharmacy and Pharmaceutical Sciences. 6 (6): 61-74.

      [21] El-Boushy, M; Sanaa, S and Abeer, H (2006): Immunological and biochemical studies onpefloxacin in broilers infected with E. coli. Proc. of 8th Sci. Vet Med Zag Conf.PP: 55-59

      [22] El-Hewaity M, Abd El Latif A, Soliman A, Aboubakr M. (2014) Comparative Pharmacokinetics of Cefquinome (Cobactan 2.5%) following RepeatedIntramuscular Administrations in Sheep and Goats. J Vet Med.2014:949642. https://doi.org/10.1155/2014/949642.

      [23] EL-Kadeem, A.M. (2005): Pharmacological studies of gentamicin and ciproflo-xacin in colibacillosis in chickens. MVSc. Thesis, Fac. Vet. Med., Zagazig Uni.

      [24] El-Nemr, AE. (2011):Efficacyof florfenicol on E coli infection in chicken.Thesis presented to Fac. of Vet. Med.Cairo. Uni. for the degree of Ph.D.

      [25] El-Sissi, Ashgan, F. and Mohamed, SH (2011): Impact of synbiotic on immune response of broilers against NDV vaccines. Global J of Biot and Bioch. 6 (4): 186-191.

      [26] Foley, E. and Farrell, P. (2003): Nitric oxide contributes to induction of innate immune responses to Gr-ve bacteria in Drosophila. Foley & Farrell- Genes &Develo. 17: 115- 125.https://doi.org/10.1101/gad.1018503.

      [27] Godbole, P. (2017): evaluation of prophylactic and therapeutic efficacy of curcumin against escherichia coli-induced infection in broiler chicks. Master Thesis Submitted to Maharashtra Animal and Fishery Sciences Uni, Nagpur (India) (Vet Pharmacology).

      [28] Haq, K.; Khan, S. and Nabi, G. (2015) Efficacy of Clarithromycin and Cefpod-oxime Against Colibacillosis in Pigeons. Ame- Eurasian J of Toxic Sci. 7(2):72-82.

      [29] Hassan, M. and Hassanein, Z. (1999): Effect of E coli on the immune response of chickens to Newcastle virus vaccine. J. Egypt. Vet. Med. Assoc. 59: 75-91.

      [30] Hegazy, A.; Eid, A.; Abdel-Aleem, I.; Youssef, B.; Nasser, A. and Hany, A. (2006) studies on E. coli infection in chickens and immune response after Newcastle disease vaccination. 8th Sci. Vet. Med. Zag. , Conf. 231-239

      [31] Henry, R.; Cannon, D. and Winkelman, J. (1974) Clinical Chemistry Principals and tec- hniques p 437 – 440, Harper and Row, Hagerstown.

      [32] Huang, H. and Matsumoto, M. (2000): Non-specific innate immunity against E coli infection in chickens induced by Newcastle disease vaccine. Avian Disease. 44:790-796.https://doi.org/10.2307/1593050.

      [33] Jain, N (1986): Schalm’s veterinary Haematology 4th Ed p. 55-96 Lee and Febiger, Philadelphia, U.S.A.

      [34] James, E. (1993). Martindale, the extra pharmacopoeia, Royal Pharmaceutical Society, London. 30th Ed;

      [35] Jeon, W.; Lee, E. and Lee, Y (2008) Protective efficacy of Newcastle vaccines in chickens against a recent Korean epizootic strain. J of Vet Sci. 9(3): 25-30. https://doi.org/10.4142/jvs.2008.9.3.295.

      [36] Kaneko J (1989) Biochemistry of domestic animal Acad. Pres Inc New York.

      [37] Kumari, M. (2016) Pathological and immunological studies on Escherichia coli infection in broiler chickens fed on Withaniasomnifera and Aloe vera extracts. Ph.D. thesis submitted to Lala Lajpat Rai Uni of Vet Sci.

      [38] La Ragione R. and Woodward M. (2002) Virulence factors of E. coli serotypes associated with avian coli septicemia. Res. Vet. Sci. 73:27–35. https://doi.org/10.1016/S0034-5288(02)00075-9.

      [39] Mohamed HMA and Younis W (2018) Trials on the Role of Probiotics in Colonization and Immune Response of Broilers Challenged with E Coli K88. Alex J of Vet Sci. 58 (1): 48-56https://doi.org/10.5455/ajvs.297887.

      [40] Mohamed, D. (2015) efficacy and residues of doxycycline in ducks infected with E. coliMVSc.Thesis (Pathology Fac. of Vet. Med., Zag Uni.)

      [41] Mwafy, RM. (2000): Pharmacological profile of concurrent use of some antimicrobials in chickens. MVSc Thesis presented to Fac. Vet. Med., Zag Unvi.

      [42] Nakamura, K. Ueda, H. Tanimura, T. and Noguchi, K. (1994):Effect of mixed live vaccine (Newcactle disease) and Mycoplasma gallisepticum on the chicken respiratory tract and on Escherichia coli infection. J. Comp. pathol. 111:33-42.https://doi.org/10.1016/S0021-9975(05)80109-4.

      [43] Otaki Y. (1995) Poultry disease control in Japan. Asian Livestock. 20: 65–67.

      [44] Petrie,A and Watson,P.(1999):“ Statistics for Veterinary and Animal Science.“1stEd. PP. 90 – 99. The Black well Sc. Ltd. United Kingdom.

      [45] Rajaraman, V.; Nonnecke, B.; Franklin, S. and Horst, R. (1998): Effect of vitamins A and E on nitric oxide production by blood mononuclear leukocytes from neonatal calves fed milk replacer. J Dairy Sci., 81:378-385https://doi.org/10.3168/jds.S0022-0302(98)75892-8.

      [46] Ramadan, A and Attia, E (2003): Natural killing molecules in cervical mucus of buffaloes during estrous cycle. 7th Sci. Cong. Egypt. Soci. for cattle dis, Assiut.

      [47] Rosario, C; Lopaz, A.; Tellez, I.; Navarro, O and Eslava, C (2004): Serotyping and virulence genes detection in E coli isolated from fertile and infertile eggs, dead-in-shell embryos and chickens with yolk sac infection. Avian Dis; 48 (4):81-89.https://doi.org/10.1637/7195-041304R.

      [48] Rouse, B.; Babiuk, L. and Henson, P. (1980): Neutrophils in antiviral inhibition of virus replication of mediators produced by bovine neutrophils.J.Inf. Dis 141(2)23– 32https://doi.org/10.1093/infdis/141.2.223.

      [49] Schltz, L (1987): Methods in Clinical Chemistry.The CV Mosby cost Louis, 42- 46

      [50] Shawky, NA. (2006): Antibacterial efficacy of cefoperazone and sulbactam in chickens. Thesis presented to Fac. Vet. Med. Zag. Uni. for Ph.D. degree "pharmacology"

      [51] Thomson, T; Quay, J and Webber, J (1984): Cephalosporin group of antimicr-robial drugs. J. Am. Vet. Med. Ass. 185(10):1109– 1114

      [52] Tizard, I. (1996): "Veterinary immunology. An Introduction." 5th Ed., WB Saunders Comp, A division of Harcourt Brace Comp, London, Toronto, Monterialo, Sedny, Tokyo

      [53] Wang, X; Zhou, Q; Shen, J and Yang, X (2015): Effect of Newcastle disease vaccine immunization on growth performance and immune response of broilers. J. Anim. Sci. and Bio, 6(1): 20. https://doi.org/10.1186/s40104-015-0019-y.

      [54] Wilson, A and Gisvold, T (1982): Textbook of organic medicinal and pharmaceutical chemistry. 8th edition,

      [55] Woldehiwet, Z and Rowan, T (1990): effects of age of calves on phagocytosis and killing of Staph aureus by polymorph nuclear leucocytes. Br. Vet J. 146: 65– 70.https://doi.org/10.1016/0007-1935(90)90009-R.

      [56] Yassin, EM. (2012): SomeImmunochemical studies on microbial exposure of Rabbits. M.Sc. Thesis Zagazig Uni. Fac. of Vet. Med. Dep. of Biochemistry.

      [57] Zaki, M; Fawzy, O and Osfor, M (2012): Effect of E. coli 0157 on Baladi Broiler Chicken and some Biochemical studies. Life Sci. J. 9(1): 91-94.

  • Downloads

  • How to Cite

    Elkomy, A., Aboubakr, M., Emam, E., & Kassem, M. (2019). Studies on the effects of cephradine and colibacellosis on immunological status of broiler chicken vaccinated with newcastle virus vaccine. International Journal of Pharmacology and Toxicology, 7(2), 17-21. https://doi.org/10.14419/ijpt.v7i2.29020