Antioxidant-mediated neuro-protective and GABAergic calming effect of Stephania japonica
Keywords:AChE, Anxiolytic-Like Effect, GABA-Agonistic Action.
Stephania japonica, a tropically-habituated plant is widely distributed in Bangladesh. Traditionally, it has been used in the treatment of inflammation, asthma, fever, sleep disturbance, painful conditions, and rheumatism. However, scientific evidence of its biological activities are very limited. This study evaluated neuroprotctivity along with a possible anxiolytic-like effect of the methanol leaf extract of S. japonica (MSJ). The antioxidant test was performed by using the scavenging capacity of hydroxyl (â—OH) and nitric oxide (â—OH) radicals; and an inhibition of lipid peroxidation assay. In vitro, egg albumin denaturation (IELD) and anti-acetylcholinesterase (anti-AChE) tests were performed to evaluate the anti-inflammatory and anti-cholinesterase activity, respectively. Additionally, a possible anxiolytic action of the MSJ was investigated in Swiss mice by taking diazepam (DZP) and flumazenil (FLU) as gamma amino butyric acid (GABA) receptor agonist and antagonist, respectively. The MSJ concentration (50-200 µg/mL)/dose (50-200 mg/kg, oral)-dependently exhibited antioxidant, anti-inflammatory, anti-AChE and anxiolytic-like effects. The MSJ also increased the activities of the standards used in antioxidant and anti-AChE (trolox), anti-inflammatory (acetyl salicylic acid), and anxiolytic (DZP: agonist) test. The MSJ exhibited antioxidant and anti-inflammatory activities. It also potentiated the action of DZP, while antagonizing the effect of the FLU, suggesting a possible GABAergic, DZP-agonistic anxiolytic-like action in experimental animals. S. japonica may be one of the good sources of neuroprotective phytochemicals.
 Barrera G. 2012. Oxidative Stress and Lipid Peroxidation Products in Cancer Progression and Therapy. ISRN Oncol. 2012:137289. https://doi.org/10.5402/2012/137289.
 Carroll AR, Arumugan T, Redburn J, et al. 2010. Hasubanan alkaloids with Î´-opioid binding affinity from the aerial parts of Stephania japonica. J Nat Prod. 73:988-991. https://doi.org/10.1021/np100009j.
 Costa JP, Oliveira GAL, Almeida AAC, Islam MT, Sousa DP, Freitas RM. 2014. Anxiolytic-like effects of phytol: Possible involvement of GABAergic transmission. Brain Res 1547: 34-42. https://doi.org/10.1016/j.brainres.2013.12.003.
 Del Valle-Laisequilla CF, Flores-Murrieta FJ, Granados-Soto V, Rocha-Gonzalez HI, Reyes-Garcia G. 2012. Ketorolac tromethamine improves the analgesic effect of hyoscine butylbromide in patients with intense cramping pain from gastrointestinal or genitourinary origin. Arzneimittelforschung. 62:603-608. https://doi.org/10.1055/s-0032-1327678.
 Esterbauer H, Cheeseman KH. 1990. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Meth Enzymol. 186:407-421. https://doi.org/10.1016/0076-6879(90)86134-H.
 Girish TK, Vasudevaraju P, Prasada Rao UJS. 2012. Protection of DNA and erythrocytes from free radical induced oxidative damage by black gram (Vigna mungo L.) husk extract. Food Chem Toxicol. 50:1690-1696. https://doi.org/10.1016/j.fct.2012.01.043.
 Islam MT, Freitas RM, Oliveira GLS, et al. 2014. Neuropharmacological screenings of hydroalcoholic fractions of Urena lobata L. World J Pharm Pharmaceut Sci. 3:62-71.
 Islam MT, Mata AMOF, Aguiar RPS, Paz MFCJ, Alencar MVOB, Melo-Cavalcante AAC. 2016a. Therapeutic Potential of Essential Oils Focusing on Diterpens. Phytother Res. 30:1420-1444. https://doi.org/10.1002/ptr.5652.
 Islam MT, Streck L, Paz MFCJ, et al. 2016b. Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity. Int Arch Med. 9:1-15. https://doi.org/10.3823/1941.
 Islam MT, Silva CB, Alencar MVOB, et al. 2016c. Diterpenes: Advances in Neurobiological Drug Research. Phytother Res. 30:915-928. https://doi.org/10.1002/ptr.5609.
 Islam MT. 2016. Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders. Neurol Res. https://doi.org/10.1080/01616412.2016.1251711.
 Jahan R, Khatun MA, Nahar N, et al. 2010. Use of Menispermaceae family plants in folk medicine of Bangladesh. Adv Nat Appl Sci. 4:1-9.
 Kirtikar KR, Basu BD. 1958. Indian Medicinal Plants. International Book Dis- tributors, India.
 Lowe IP, Robins E, Eyerman GS. 1958. The flourimetric measurement of glutamic decarboxylase and its distribution in brain. J Neurochem. 3:8-16. https://doi.org/10.1111/j.1471-4159.1958.tb12604.x.
 Lu Y, Zhang H, Meng X, et al. 2010. A validated HPLC method for the determination of GABA by pre-column derivatization with 2, 4-dinitroflurodinitrobenzene and its application to plant GAD activity study. Analytical Lett. 43:2663-2671. https://doi.org/10.1080/00032711003763558.
 Marcocci I, Marguire JJ, Droy-lefaiz MT, et al. 1994. The nitric oxide scavenging properties of Ginkgo biloba extract. Biochem Biophys Res Comm. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764.
 Matsui M, Kabashima T, Ishida K, Takebayashi T, Watanabe Y. 1982. Alkaloids of the leaves of Stephania japonica. J Nat Prod. 45:497-500. https://doi.org/10.1021/np50022a026.
 Mestres G, Santos CF, Engman L, et al. 2015. Scavenging effect of trolox released from brushite cements. Acta Biomaterialia. 11:459-466. https://doi.org/10.1016/j.actbio.2014.09.007.
 Moniruzzaman M, Hossain MS, Bhattacharjee PS. 2016. Evaluation of antinociceptive activity of methanolic extract of leaves of Stephania japonica Linn. J Ethnopharmacol. 186:205-208. https://doi.org/10.1016/j.jep.2016.04.008.
 Ota K, Oishi N, Ito K. 2015. Effects of imaging modalities, brain atlases and feature selection on prediction of Alzheimerâ€™s disease. J Neurosci Meth. 256:168-183. https://doi.org/10.1016/j.jneumeth.2015.08.020.
 Pohanka M, Hrabinova M, Kuca K, et al. 2011. Assessment of Acetylcholinesterase Activity Using Indoxylacetate and Comparison with the Standard Ellmanâ€™s Method. Int J Mol Sci. 12:2631-2640. https://doi.org/10.3390/ijms12042631.
 Rahman MA, Uddin SB, Wilcock CC. 2007. Medicinal plants used by Chakma tribe in hilltracts districts of Bangladesh. Indian J Tradit Knowl. 6:508-517.
 Rahman MH, Alam MB, Chowdhury NS, et al. 2011. Antioxidant, analgesic and toxic potentiality of methanolic extract of Stephania japonica (thunb.) Miers. Leaf. Int J Pharmacol. 7:257-262. https://doi.org/10.3923/ijp.2011.257.262.
 Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen. 10:1003-1008. https://doi.org/10.1093/carcin/10.6.1003.
 Rye DB, Bliwise DL, Parker K, et al. 2012. Modulation of Vigilance in the Primary Hypersomnias by Endogenous Enhancement of GABAA Receptors. Sci Transl Med. 4:151-161. https://doi.org/10.1126/scitranslmed.3004685.
 Seraj S, Jahan FI, Chowdhury AR, et al. 2013. Tribal formulations for treatment of pain: a study of the bede community traditional medicinal practitioners of porabari village in Dhaka district, Bangladesh. Afr J Tradit Complement Altern Med. 10:26-34.
 Tan KR, Rudolph U, LÃ¼scher C. 2014. Hooked on benzodiazepines: GABAA receptor subtypes and addiction. Trends Neurosci. 34:188-197. https://doi.org/10.1016/j.tins.2011.01.004.
 Triana-Vidal LE, Carvajal-Varona SM. 2013. Protective effect of galantamine against oxidative damage using human lymphocytes : A novel in vitro model. Arch Med Res. 44:85-92. https://doi.org/10.1016/j.arcmed.2013.01.001.
 Uddin SMN, Amin MN, Shahid-Ud-Daula AFM, et al. 2014. Phytochemical screening and study of antioxidant and analgesic potentials of ethanolic extract of Stephania japonica Linn. J Med Plant Res. 8:1127-1133. https://doi.org/10.5897/JMPR2014.5443.
 Warner TA, Kang JQ, Kennard JA, et al. 2015. Low brain ascorbic acid increases susceptibility to seizures in mouse models of decreased brain ascorbic acid transport and Alzheimerâ€™s disease. Epilepsy Res. 110:20-25. https://doi.org/10.1016/j.eplepsyres.2014.11.017.
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