Mineralisation of natural rubber (poly cis 1-4 isoprene) by co-cultured bacterial strains isolated from rubber plantation area

  • Authors

    • Manasa Muralidharan Department of Biotechnology, Stella Maris College
    • Veena Gayathri Krishnaswamy
    2016-01-20
    https://doi.org/10.14419/ijbr.v4i1.5552
  • Bacillus cohnii, Brevundimonas naejangsanensis, Natural Rubber, Mineralisation 16s r RNA sequencing.
  • Abstract

    Objective: To isolate Natural Rubber degrading Bacterial co-cultures from rubber plantation soil and to characterize and identify the organisms by 16s r RNA sequencing.

    Methods: Cocultures of the bacteria were isolated from the contaminated site by enrichment culture technique. Plate assay method and liquid assay method by using Mineral Salt Medium was followed for screening of bacteria for its capacity to mineralize Natural rubber. Degradation was confirmed by Spectrophotmetric and Fourier Transform Infra-Red (FTIR) studies.Natural rubber degraded by the cocultures were studied at different concentrations and the physico-chemical analysis were optimized (pH, temperature, carbon and nitrogen sources)

    Results: Isolated organism was identified as Bacillus cohnii and Brevundimonas naejangsanensis. The co-cultures were able to utilize the Natural rubber which was confirmed by Spectrophotometric and FTIR studies. From the current study it was evaluated that Natural rubber was mineralized up to 50 % where optimum concentration was 10 %.

    Conclusion: From current investigation, it can be concluded that our isolated bacterial cocultures Bacillus cohnii and Brevundimonas naejangsanensis have the capacity to mineralize Natural rubber and hence such isolated cocultures can be used in removal of from waste Natural rubber products in the environment.

  • References

    1. [1] Berekaa M.M.,Barakaat A., El-Sayeed S.M. & El-Aassar S. A. (2005) Degradation of Natural Rubber by Achromobacter sp. NRB and Evaluation of culture conditions, Polish Journal of Microbiology . 54, (1), 55-62.

      [2] Cui Q., Wang L., Huag Y., Liu Z. & Goodfellow M. (2005), Nocardiajiangxiensis sp. nov.and Nocardiamiyunensis sp. nov., isolated from acidic soils. International Journal of Systematic and Evolutionary Microbiology. 55: 1921-1925. http://dx.doi.org/10.1099/ijs.0.63644-0.

      [3] Lee, K.M., Gimore, D.F. & Huss M.J. (2005), Fungal Degradation of the Bioplastics PHB, Journal of Polymer and the environment. 13, DOI 10.1007/s10924-005-4756-4.

      [4] Rose K. & Steinbuchel A. (2005) Biodegradation of natural rubber and related compounds: Recent insights into a hardly understood catabolic capability of microorganisms, Applied and Environmental Microbiology. 71: 2803-2812. http://dx.doi.org/10.1128/AEM.71.6.2803-2812.2005.

      [5] Rose K., Tenberge K. B. & Steinbuchel A. (2005). Identification and characterization of genes from Streptomyces sp. strain K30 responsible for clear zone formation on natural rubber latex and poly (cis-1, 4-isoprene) rubber degradation, Biomacromolecules 6, (1), 180- 188. http://dx.doi.org/10.1021/bm0496110.

      [6] Yamamura H., Hayakawa M., Nakagawa Y., Tamura T., Kohno T., Komatsu F. & Limura Y. (2005), Nocardia takedensis sp. nov., isolated from moat sediment and scumming activated sludgeâ€. International Journal of Systematic and Evolutionary Microbiology. 55: 433-436. http://dx.doi.org/10.1099/ijs.0.63189-0.

      [7] Ibrahim E. M. A., Arenskotter M., Luftmann H. & Steinbuchel A. (2006). “Identification of poly (cis-1, 4- isoprene) degradation intermediates during growth of moderately thermophilic Actimycetes on rubber and cloning of a functional ICP homologue from Nocardia farcinica strain EIâ€. Applied Environmental Microbiology 72: 3375-3382. http://dx.doi.org/10.1128/AEM.72.5.3375-3382.2006.

      [8] Roy R.V., Das M., Banerjee R. & Bhowmick A. K. (2006). Comparative studies on cross linked and uncross linked natural rubber biodegradation by Pseudomonas sp. Bio resource Technology. 97, 18, 2485-2488. http://dx.doi.org/10.1016/j.biortech.2005.09.024.

      [9] Roy R.V., Das M., Banerjee R. & Bhowmick A. K. (2006). Comparative studies on cross linked and uncross linked natural rubber biodegradation by Pseudomonas sp. Bio Resource Technology, 97, 18, 2485-2488. http://dx.doi.org/10.1016/j.biortech.2005.09.024.

      [10] Yu L., Dean K. & Li L. (2006). Polymer blends and composites from renewable resources. Progress in Polymer Science. 31, 6, 576-602. http://dx.doi.org/10.1016/j.progpolymsci.2006.03.002.

      [11] Seo S. J., Keum Y. S., Harada R. M. & Li Q. X. (2007). Isolation and characterization of bacteria capable of degrading polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides from PAH-contaminated soil in Hilo, Hawaii. Journal of Agricultural and Food Chemistry, 55, (14), 5383-5389. http://dx.doi.org/10.1021/jf0637630.

      [12] Seo S.J., Keum Y.S., Harada R.M. & Li Q. X. (2007). Isolation and characterization of bacteria capable of degrading polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides from PAH-contaminated soil in Hilo, Hawaii. Journal of Agricultural and Food Chemistry.55, (14), 5383-5389. http://dx.doi.org/10.1021/jf0637630.

      [13] Warneke S., Arenskotter M., Tenberge K. B. & Steinbuchel A. (2007). Bacterial degradation of poly (trans-1, 4-isoprene) (Guttapercha). Microbiology 153, (2), 347- 356. http://dx.doi.org/10.1099/mic.0.2006/000109-0.

      [14] Aamer Ali Shah., FariahHaasan, Abdul Hameed., & Safia Ahmed (2008) Biodegradation of plastics: A Comprehensive Review, Biotechnology Advances 246-265.

      [15] Cherian E. & Jayachandran K. (2009). Microbial degradation of natural rubber latex by a novel species of Bacillus sp. SBS 25 isolated from soil, International Journal of Environmental Research, 3, (4), 599-604.

      [16] Tanrattanakul V. & Chumeka W.(2009). Effect of potassium persulfate on graft copolymerization and mechanical properties of cassava starch/natural rubber foams. Journal of Applied Polymer Science.116, (1), 93-105. http://dx.doi.org/10.1002/app.31514.

      [17] Wang Z. F., Peng Z., Li S. D., Lin H., Zhang K. X., She X. D. & Fu X. (2009). The impact of esterification on the properties of starch/natural rubber composite. Composites Science and Technology. 69, (11-12), 1797-1803. http://dx.doi.org/10.1016/j.compscitech.2009.04.018.

      [18] Ziaullah Shah, Aamer Ali Shah, Abdul Hameed & Fariha Hasan. (2009). Effect of pretreatments on enhanced degradation of polyisoprene rubber by newly isolated Bacillus sp. Strain S10. Journal of the Chemical Society of Pakistan. 31, (4), 638-646.

      [19] Desmond Threadingham, Werner Obrecht, Wolfgang Wieder, Gerhard Wachholz and Rüdiger Engehausen. (2011) "Rubber, 3. Synthetic Rubbers, Introduction and Overview" in Ullmann'sEncyclopedia of Industrial Chemistry. Weinheim. http://dx.doi.org/10.1002/14356007.a23_239.pub5.

      [20] SirimapornWatcharakul, KamontamUmsakul, Brian Hodgson, WannapaChumeka, & Varaporn Tanrattanakul. (2012). Electronic Journal of Biotechnology, 15, 1. ISSN: 0717-3458

      [21] Nayanashree.G & Thippeswamy.B, (2013) Natural Rubber Degradation By Aspergillus niger and Penicillium sp. International Journal of Recent Scientific Research 4,(9),1337- 1341.

      [22] Nayanashree.G, Thippeswamy.B & Krishnappa.M (2014) Natural Rubber Biodegradation by Cladosporium fulvum and Enzymes responsible for Biodegradation International Journal of Advanced Research, 2, (4), 1206-1212.

      [23] Nayanashree.G, Thippeswamy.B, & Krishnappa.M (2014) Enzymatic studies on Natural rubber Biodegradation by Bacillus pumilus, International Journal of Biological Research, 2 (2) 44-4.

      [24] Abd ElLatif Heshama , Nadia H. Mohamedb, Mady A. Ismail & Ahmed A.M. Shoreit Microbiology, 84, (3),351–358.

  • Downloads

  • How to Cite

    Muralidharan, M., & Krishnaswamy, V. G. (2016). Mineralisation of natural rubber (poly cis 1-4 isoprene) by co-cultured bacterial strains isolated from rubber plantation area. International Journal of Biological Research, 4(1), 1-9. https://doi.org/10.14419/ijbr.v4i1.5552

    Received date: 2015-11-18

    Accepted date: 2015-12-12

    Published date: 2016-01-20