Study on heavy metals biosorption ability of Saccharomyces cerevisiae

  • Abstract
  • Keywords
  • References
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  • Abstract

    Potential Saccharomyces cerevisiae was isolated from paper effluent for bioremediation of heavy metals. Morphological studies, physiological tests and molecular characterization confirmed isolated yeast colony was S. cerevisiae. S. cerevisiae has showed high biosorption of Cd2 + (67%), followed by Pb2+ (61%)> Ni2+ (64%)> Cr6+ (63%)> Cu2+ (57%)> Zn2+ (53%). SEM image of heavy metals treated S. cerevisiae showed patches of accumulated heavy metals and EDs spectrum of S. cerevisiae treated with heavy metals contained metal peaks. Elemental analysis by EDS confirmed that ion exchange mainly involved in heavy metals biosorption by S. cerevisiae. Optimization of parameters made maximum biosorption of heavy metals. The pH 4.0-5.0, temperature 20-25C and contact time of 60 minute was found optimal for heavy metals biosorption. The metal biosorption was found maximum in single metal system compared to multi metal ions. Pre-treatment method enhanced the metal biosorption. Sorption isotherm studies fit into the model of Langmuir isotherm compared to Freundlich isotherm.

    Keywords: Biosorption, Heavy metals, Optimization, Saccharomyces cerevisiae, Sorption isotherm.

  • References

    1. Abhishek M, Durba D, Sushil KM, Raktim B, Tapan KD, Naoual B & Anisur, RK (2010), Tolerance of arsenate-induced stress in Aspergillus niger, a possible candidate for bioremediation. Ecotoxicology and Environmental Safety 73, 172-182.
    2. Adeyemi AO (2009). Bioaccumulation of arsenic by fungi. American Journal of Environmental Science 05, 364-370.
    3. Ahluwalia SS & Goyal D (2007), Microbial and plant derived biomass for removal of heavy metal from wastewater. Bioresource Technology 98, 2243-2257.
    4. Akar T & Tunali S (2006), Biosorption characteristics of Aspergillus flavus biomass for removal of Pb(II) and Cu(II) ions from an aqueous solution. Bioresource Technology 97, 1780-1787.
    5. Aneja KR (2003), .Experiments in Microbiology, Plant Pathology and Biotechnology, New age international publishers. 04, 257-290.
    6. Chen C & Wang J (2007), Response of Saccharomyces cerevisiae to lead ion stress. Applied Microbial Biotechnology 74, 683-687.
    7. Choudhary S & Sar P (2009), Characterization of a metal resistant Pseudomonas sp. isolated from uranimum mine for its potential in heavy metal (Ni2+, Co2+, Cu2+, and Cd2+) sequestration. Bioresource Technology 100, 2482-2492.
    8. Cooke WB, Phaff HJ, Miller MW, Shifrine M & Knapp EP (1960), Yeasts in polluted water and sewage. Mycologia 52, 210-230.
    9. Dostalek P, Patzak M & Matejka P (2004), Influence of specific growth limitation on biosorption of heavy metals by Saccharomyces cerevisiae. International Biodeterioration and Biodegradation 54, 203-207.
    10. Engl A & Kunz B (1995), Biosorption of heavy metals by Saccharomyces cerevisiae: effect of nutrient conditions. Journal of Chemical Technology and Biotechnology 63, 257-261.
    11. Goldstein JI, Newbury, DE, Joy, DC, Lyman CE, Echlin O, Lifshin E, Sawyer L & Michael JR (2003), Scanning electron microscopy and X-ray microanalysis. 3rd Edition, Kluwer Academia/Plenum Publishers, New York.
    12. Ho YS, Ng JCY & Mckay G (2000), Kinetics of pollutant sorption by biosorbents: a review. Separation and Purification Methods 29, 189-232.
    13. Huma R, Haq N & Bhatti TM (2001), Bioleaching studies of bauxite ore using Aspergillus nige. Journal of Biological Sciences 01, 501-504.
    14. Iqbal A, Shaheen Z & Farah A (2005), Heavy metal biosorption potential of Aspergillus and Rhizopussp. Isolated from wastewater treated soil. Journal of Applied Sciences and Environmental Management 09, 123-126.
    15. Jaeckel P, Krauss GJ & Krauss G (2005), Cadmium and zinc response of the fungi Heliscus lugdunensis and Verticillium cf. Alboatrum isolated from highly polluted water. Science of the Total Environment 346, 274-279.
    16. Johncy MR, Hemambika B, Hemapriya J & Rajesh KV (2010), Comparative assessment of heavy metal removal by immobilized and dead bacterial cells: A biosorption approach. African Journal of Environmental Science and Technology 04, 77-83.
    17. Kambe HH, Sugawara A, Yoda K, Kitamoto K & Yamasaki M (1997), Isolation and characterization of nickel-accumulating yeasts. Applied Microbial Biotechnology 48, 373-378.
    18. Kang SY, Lee JU & Kim KW (2007), Biosorption of Cr (III) and Cr (VI) onto the cell surface of Pseudomonas aeruginosa, Biochemical Engineering Journal 36, 54-58.
    19. Kapoor A, Viraraghavan T & Cullimor DR (1999), Removal of heavy metals using the fungus Aspergillus niger. Bioresource Technology 70, 95-104. (98)00192-8.
    20. Kurtzman CP (1988), Identification and taxonomy of yeast. In: Kirsop, B.E. and C.P. Kurtzman (Eds). Living Resources for Biotechnology: Yeast. Cambridge University Press, New York: 98-140.
    21. Madhu P, Srivastava & Neeta S (2013), Biosorption of copper zinc and chromium from aqueous solution by fungal strains. Journal of Chemistry and Chemical Science 03, 131-139.
    22. Melgar MJ, Alonso J & Garcia MA (2007), Removal of toxic metals from aqueous solutions by fungal biomass of Agaricus Macrospores. Science of the Total Environment 385, 12-19.
    23. Marjeta R, Neza C & Peter R (2002), Identification and characterization of yeast isolates from pharmaceutical waste water. Food Technology and Biotechnology 40, 79-84.
    24. Nur LI, Nur AIMZ & Soon GT (2011), Tolerance and biosorption of copper (Cu) and lead (Pb) by filamentous fungi isolated from a freshwater ecosystem. Journal of Environmental Sciences 23, 824-830.
    25. Ozer a & Ozer D (2003), Comparative study of the biosorption of Pb (II), Ni (II) and Cr (VI) ions onto Saccharomyces cerevisiae: Determination of biosorption heats. Journal of Hazardous Material 100, 219-229. (03)00109-2.
    26. Pinoa GH, Mesquitaa DE, Torema ML & Pinto GAS (2006), Biosorption of Heavy Metals by Powder of Green Coconut Shell. Separation Science and Technology 41, 3141-3153.
    27. Price MS, Classen JJ & Payne GA (2001), Aspergillus niger absorbs copper and zinc from swine wastewater. Bioresource Technology77, 41-49. (00)00135-8.
    28. Rao KR, Rashmi K, Latha JNL & Mohan PM (2005), Bioremediation of toxic metal ions using biomass of Aspergillus fumigates from fermentative waste. Indian Journal of Biotechnology 04, 139-143.
    29. Sag Y (2001), Biosorption of heavy metals by fungal biomass and modelling of fungal biosorption: a review. Separation and Purification Methods 30, 01-48.
    30. Sag Y & Kutsal T (2000), Determination of the biosorption activation energies of heavy metal ions on Zoogloea raigera and Rhizopus arrhizus. Process Biochemistry 35, 801-807. (99)00154-5.
    31. Sahni SK (2011), Hazardous metals and minerals pollution in India. Indian National Science Academy. Angkor Publishers (P) Ltd., New Delhi: 29.
    32. Saifuddin N & Raziah AZ (2007), Removal of heavy metals from industrial effluent using Saccharomyces cerevisiae (Bakers yeast) immobilized in chitosan lignosulphonate matrix. Journal of Applied Science Research 03, 2091-2099.
    33. Sarabjeet SA & Dinesh G (2007), Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresource Technology 98, 2243-2257.
    34. Shadia MA, Hoda AH & Foukia EM (2012), Acidic exo polysaccharide flocculant produced by the fungus Mucorrouxii using Beet-Molasses. Research in Biotechnology 03, 01-13.
    35. Shankar C, Sridevi D, Joonhong P, Michael D & Kaliannan T (2007), Biosorption of chromium and nickel by heavy metal resistant fungal and bacterial isolates. Journal of Hazardous Materials 146, 270-277.
    36. Shanthi J, Krubakaran CTB & Balagurunathan R (2012), Characterization and isolation of paper mill effluent degrading microorganisms. Journal of Chemical and Pharmaceutical Research 04, 4436-4439.
    37. Shazia I, Kousar P, Jawaria U, Kinat N, Noreen A, Sana A & Iftikhar A (2012), Heavy metal tolerance of filamentous fungal strains isolated from soil irrigated with industrial waste water. Biologija 58, 107-116.
    38. Smith EH (1996), Uptake of heavy metals in batch systems by a recycled iron-bearing material, Water Research 30, 2424-2434. (96)00105-4.
    39. Soares EV & Soares HMVM (2012), Bioremediation of industrial effluents containing heavy metals using brewing cells of Saccharomyces cerevisiae as a green technology: a review. Environment Science Pollution Research 19, 1066-1083.
    40. Sofyan A, Utomo R, Yusiati LM & Widyastuti Y (2000), Isolation and identification of lactic acid bacteria and Saccharomyces cerevisiaefrom natural sources as feed-silage inoculants.Proceeding: The 3rd International Conference of Indonesian Society for Lactic Acid Bacteria (3rd IC-ISLAB.). Better Life with Lactic Acid Bacteria: Exploring Novel Functions of Lactic Acid Bacteria.
    41. Srivastava S & Thakur IS (2006), Isolation and process parameter optimization of Aspergillus sp. For removal of chromium from tannery effluent. Bioresource Technology97, 1167-1173.
    42. Tsekova K & Petrov G (2002), Removal of heavy metals from aquous solution using Rhizopus delmar Mycelia in free and polyuretane-bound form. Z. Naturforsch 57, 629-633.
    43. Vara S, Avasn YM & Mukkanti K (2010), Potential fungi for bioremediation of industrial effluents. Bioresources 05, 08-22.
    44. Vijayaraghavan, K & Yeoung SY (2008), Bacterial biosorbents and biosorption.Biotechnology Advances 26, 266-291.
    45. Veglio F & Beolcini F (1997), Removal of metals by biosorption: A review. Hydrometallurgy 44, 301-316. (96)00059-X.
    46. Warren NG & Shadomy HJ (1991), Yeast of medical importance, In Balows, A., Hausler, W.J., Herrmann, K.L., Isenberg, H.D., Shadomy, H.J. (Eds.). Manual of Clinical Microbiology 5th ed. American Society for Microbiology, Washington, D.C: 617-629.
    47. Xue Q & Lu S (2008), Microstructure of ferrospheres in fly ashes: SEM, EDX and ESEM analysis. Journal of Zhejiang University SCIENCE A, 09, 1595-1600.




Article ID: 2921
DOI: 10.14419/ijbr.v2i2.2921

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