Phytochemical screening and antibacterial activity of rosmarinus officinalis l. against Escherichia coli. local isolates

  • Authors

    • Saima Johar m.phil scholer in Sardar Bahadur Khan Women's University Quetta, Pakistan
    • Shazia Irfan
    • Shehnaz Sher Ahmed
    • Rukhsana Jabeen
    2015-11-05
    https://doi.org/10.14419/ijbas.v4i4.5084
  • Aqueous Extract. Methonolic Extract, Concentrations, Phytochemical, Antibacterial.

  • Rosmarinus officinalis is a medicinal plant which belongs to family Lamiaceae. It is an evergreen bush. It was analyzed for its phytochemical constitution and antibacterial activity. For this purpose flowers, leaves and stem of aqueous and methanolic extracts were used. The tests of phytochemical screening showed that flavonoids, terpenoids, reducing sugar and saponins were present in different concentrations, while tannin was absent in all parts of plant. The antibacterial activity was determined against E. coli with different concentrations (i-e 0.5gm, 1gm and 1.5gm) kept for 24 hours and 48 hours duration. The results showed that the highest mean inhibition zone was observed in the methanolic extracts of leaves 1.5gm kept for 48 hours (28.6 ± 4.261mm).In the stem the methanolic extracts showed the highest inhibition zone with the concentration of 1.5gm (13.4 ± 1.624mm) kept for 24 hours. In the flower the highest inhibition zone in methanolic extracts was observed in 1gm (25.4 ± 2.416mm) kept for 48 hours. In the aqueous extracts the highest inhibition zone values were (17.6 ± 4.029mm, 15.8 ± 2.993mm, 14.4 ± 2.33mm) for stem, flower and leaves respectively.

  • References

    1. [1] Achakzai, A. K. K., P. Achakzai., A. Masood., S. A. Kayani and R. B. Tareen, Response of plant parts and age on the distribution of secondary metabolites on plants found in Quetta. Pak. J. Bot. 41(5): (2009) 2129-2135.

      [2] Ahmad, M., A. M. Khan., M. Zafar and S. Sultana, Treatment of common ailments by plant-based remedies among the people of district Attock (Punjab) of Northern Pakistan, Afr. J. Trad.CAM. 4(1): (2007) 112-120.

      [3] Ahmad, S., S. Koukab., M. Islam., K. Ahmad., S. Aslam., Aminullah and A. Gill, Germplasm evaluation of medicinal and aromatic plants in Baluchistan. Pak. J. Bot. 40(4): (2008) 1473-1479.

      [4] Ali, S. I, Flora of Pakistan. 192 Labiateae. (1990) 26.

      [5] Atangwho, I.J., P. E. Ebong., E. U. Eyong., I. O. Williams., M. U. Eteng and G. E. Egbung, Comparative chemical composition of leaves of some ant diabetic medicinal plants. Azadirachta indica, Vernonia amygdalina and Gongronem alatifolium .African journal of biotechnology. 8(18): (2009) 4685-4689.

      [6] Ayeni, K. F and S. A. Yahya, Phytochemical screening of three medicinal plants Neem (Azadirachita indica), Hibiscus leaf (Hibiscus rosasinensis) and spear grass leaf (Impereta cylindrical). Continental J. Pharmaceutical sciences, 4: (2010) 47-50.

      [7] Ayoola.G.A.,H.Coker.,S.Adesegun.,A.A.Bello.,K.Obaweya.,E.Ezennia,E and T. Atangbayila, Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in South Western Nigeria. Tropical journal of pharmaceutical research, 7: (2008) 1019-1024.

      [8] Baydar, H., O. Sagdic., G. Ozkan., and T. Karadogen, Antibacterial activity and composition of essential oils from Origonum, Thymbra and Satureja species with commercial importance in Turkey. Food control. 15: (2004) 169-172. http://dx.doi.org/10.1016/S0956-7135(03)00028-8.

      [9] Bibi, Y., S. Nisa., A. Waheed., M. Zia., S. Sarwar., S. Ahmed and M. F. Chaudhary, Evaluation of Viburnum foetens for anticancer and antibacterial potential and phytochemical analysis. African journal of biotechnology. 9(34): (2010) 5611-5615.

      [10] Bozin, B., N. Mimica-Dukic., I. Samojilik and E. Jovin, Antimicrobial and anti-oxidant properties of Rosemary and sage (Rosemarinus officinalis and Salvia officinalis L. Lamiaceae) essential oils. Journal of Agriculture and Food Chemistry 55: (2007) 7879-7885. http://dx.doi.org/10.1021/jf0715323.

      [11] Budka, D and N. A.Khan, The effects of Ocimumbacilicum, Thymus vulgaris, and Origanium vulgare essential oils on Bacillus cereus in rice based foods. Euorpean Journal of Biological Sciences 2(1): (2010) 17-20.

      [12] Edeoga, H. O., G. Omosun and L. C. Unche, Chemical composition of Hyptis suaveolens and Ocimum gratissimum hybrids from Nigeria. African Journal of Biotechnology. 5: (2006) 892-895.

      [13] El-Bastawesy, A. M., R. H. Mohamad and A. A. El-Refai, Chemical and biological evaluation of Rosemary (Rosemarinus officinalis L.) leaves volatile oil and its methanolic extracts. Annual Agric. Sci. 54(20): (2009) 397-415.

      [14] Faixova, Z and S. Faix, Biological effects of Rosemary (Rosemarinus officinalis Li.) essential (a review). Folia veterinaria. 52(3-4): (2008) 135-139.

      [15] Farrukh, U., H. Shareef., S. Mohammad., S. A. Ali and G. H. Rizwani. 2008. Antibacterial activity of Coccinia grandis L. Pakistan Journal of Botany 40(3): (2008) 1259- 1262.

      [16] Hac-Szymanczuk, E., E. Lipinska and M. Stasiuk, The effect of Rosemary preparation on the microbial quality and tbars value of model pork batters. Acta scientiarum Polonorum / Technologia alimentaria 10(2): (2011) 165-174.

      [17] Harisarunraj, R., K. Suresh and S. Saravanababu, Evaluation of the chemical composition of Rauwdifasu pentina and Ephedra vulgaris. Advance in Biological research. 3: (2009) 174-178.

      [18] Hussain, J., A. L. Khan., N. U. Rehman., Zainullah., F. Khan., S. T. Hussain and Z. K. Shinwari. 2009. Proximate and nutrient investigation of selected medicinal plant species of Pakistan. Pakistan journal of nutrition. 8: (2009) 620-624.

      [19] Hussain, J., F. U. Khan, M. Z. Riazullah., I. Khan., M. Zohaib., Imam- ud- din and S. M. Hussain. Nutrient Evaluation and Elemental Analysis of Four selected Medicinal Plants of Khaiber Pakhtoon Khwa, Pakistan. Pakistan Journal of Botany. 43: (2011) 427-434.

      [20] Jalacic, S., L. N. Beatovic and A. Vujaosevic, The effect of natural biostimulators and slow disintegrating fertilizers on the quality of rosemary seedlings. Journal of Agricultural sciences.52: (2007) 85-94.

      [21] Khafagi, I., A. Dewedar and S. Farouk, In-vitro cytotoxicity and anti-microbial activities of some common essential oils. Egyptain Journal of biology. 2: (2000) 20-27.

      [22] Khan, A. S., I. Ilahi., N. Hunar and H. Bibi, Preliminary phytochemical screening of some plants of ethanobotanical importance from district Gilgit, Northern, areas, Pakistan. Pakistan Journal of plant Sciences 15(1): (2009) 15-18.

      [23] Kiarostemic, K.H., R. Mohseni and A. Saboor, Biochemical changes of Rosemarinus officianalis under salt stress. Journal of Stress Physiology and Biochemistry. 6: (2010) 114-122.

      [24] Kochar, A., m. Nagi and Sachdeva, Proximate composition, available carbohydrates, dietary fiber and anti-nutritional factors of selected traditional medicinal plants. Journal of Human Ecology. (3): (2006) 195-199.

      [25] Larson, R. A, Naturally occurring anti-oxidants, Boca Raton, (1997) 189.

      [26] Mierici, I.D, Phytochemical study of some active principles with antioxidant action from the Rosemarinus officinalis and Salvia officinalis species. (2009) 79-83.

      [27] Mohsenzadeh, M, Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium. Pakistan journal of biological sciences. 10(20): (2007) 3693-3697.

      [28] Muanda, F.N., J. Bouayed., A. Dijilani., C. Yao., R. Soulionani and A. Dicko. Chemical composition and cellular evaluation of antioxidant activity of Desmodium adscendens leaves. Evidence based complementary and alternative medicines (2011).

      [29] Mushtaq, A., Q. Rehmatullah., A. Mohammad., A. K. Mir and Z. Mohammad, Traditional herbal remedies used for the treatment of diabetes from district Attock (Pakistan). Pakistan Journal of Botany 41(6): (2009) 2777-2782.

      [30] Nasir, E and S. I. Ali, Flora of West Pakistan, National herbarium Agriculture Research Council Rawalpindi (1999).

      [31] Nisar, M., S. A. Tariq and I. Ullah, Nutritional levels of Indigofera gerdiana Wall and Crataegus songrica K. Koc., Pakistan Journal of Botany 41(3): (2009) 1359-1361.

      [32] Okwu, D and C. Joseph, Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology. 5(4): (2006) 357-361.

      [33] Ouwehand, A. C., K. Thhonen., H. Kehunen., S. Peuranen., H. Schulze and N. Rautonen, In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota veterinarni Medicina. 5: (2010) 71-78.

      [34] Parekh, J and S. V. Chanda, In vitro anti-microbial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology 31: (2007) 53-58.

      [35] Patel, G., S. Nayak and S. Shrivastava, Physical evaluation and qualitative chemical examination of methanolic extracts of Neriumindicum. Inter Jeurs Trends Sci Tech. 1(2): (2010) 32-36.

      [36] Rasool, R., B. A. Ganai., S. Akbar., Z. N. Kamili and A. Masood, Phytochemical screening of Prunella vulgaris L. An important medicinal plant of Pakistan. Pak. J. Pharma. Sci. 23: (2010) 399-402.

      [37] Rozman, T and B. Jersek, Antimicrobial activity of Rosemary extracts (Rosemarinus officianalis L.) against different species of Listeria (2009).

      [38] Siddique, S., A. Verma., A. A. Rather., F. Jabeen and M. K. Meghvansi, Preliminary phytochemicals analysis of some important medicinal and aromatic plants. Advanced in biological research. 3: (2009) 188-195.

      [39] Saikia, L. R and Upadhyaya, Antioxidant activity, phenol and flavinoid content of some less known medicinal plants of Assam. International journal of pharma and bio sciences. 2(2): (2011) 383-388.

      [40] Svoboda, K.P and J. B. Hampson, Bioactivity of essential oils of selected temperate aromatic plants. Antibacterial, antioxidant, anti-inflammatory and other related pharmacological activities (1999).

      [41] Taiz, L and E. Zeiger, Plant physiology. The Benjamin/ Cummings Pub. Co, Inc. 390 Bridge parkway, road city, California 94065. USA (1991).

      [42] Uddin, G., T. U. Rehman., M. Irfan., W. Liaqat., M. Qaisar., A. Rauf., G. Mohammad., M. S. Afridi and M. I. Choudary, Phytochemical and biological screening of seeds of Indigofera heteranthiawall. Middle East journal of scientific research. 8(1): (2011) 186-190.

      [43] Vaghasiya, Y., R. Dave and S. Chanda. 2011. Phytochemical analysis of some medicinal plants from western region of India. Research journal of Medicinal plants. 5: (2011) 567-57.

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    Johar, S., Irfan, S., Ahmed, S. S., & Jabeen, R. (2015). Phytochemical screening and antibacterial activity of rosmarinus officinalis l. against Escherichia coli. local isolates. International Journal of Basic and Applied Sciences, 4(4), 413-421. https://doi.org/10.14419/ijbas.v4i4.5084