Comparison of screening tests with urine culture in urinary tract infection

  • Authors

    • Shobha K.L Department of Microbiology Melaka Manipal Medical College Manipal Campus Manipal University Manipal
    • Amita Rao Department of Microbiology Melaka Manipal Medical College Manipal Campus Manipal University Manipal
    • Shilpa K Department of Microbiology Kasturba Medical College,Manipal, Manipal University Manipal
    • Ramachandra L Department of Surgery Kasturba Medical College,Manipal, Manipal University Manipal
    2014-10-17
    https://doi.org/10.14419/ijm.v2i2.3607

  • Introduction: Urinary tract infection (UTI) is an important health care problem affecting millions every year. Evaluation of suspected UTI includes history, physical examination and laboratory investigations. Urine analysis for presence of pus cells, bacteria and culture are important in the adequate management of UTIs. Aim of the study was to analyze the screening tests with culture method in the diagnosis of UTI.

    Materials and methods: 100 turbid midstream urine specimens collected in a sterile wide mouthed container were included in the study. Mid-stream urine specimens collected from both inpatients and outpatients attending a tertiary care hospital were included in the study. Urine specimen was subjected to Gram’s staining and catalase tests and inoculated into culture medium. Semi-quantitative culture technique was followed. Organisms were identified for significant bacteriuria.

    Results: Samples collected from 40 years of age and  from patients more than 40 years of age were 64% and 51% were male patients. Gram’s stain showed 59% of samples with organisms. Pus cells were seen in 54% of the smears and all the samples had significant growth in the medium Catalase positive was 53%, all the 53 strains had growth on culture plate. E. coli was the commonest organism isolated on the culture plate. Thus screening tests like Gram’s stain, pus cells detection and catalase test can be used as a rapid method to detect urinary tract infection and to start the antibiotic therapy though culture remains the gold standard method for detection of urinary tract infection.

    Keywords: Gram’s Staining, Pus Cells, Catalase Test, Culture Medium, Significant Bacteriuria.

  • References

    1. Yan-Jun Shang, Quin- Qian Wang, Jian-Rong Zhang, Yu-Lian Xu, Wei-Wei Zhang, Yan Chen, Ming-Li Gu, An-Mei Deng: Systematic review and meta-analysis of flow cytometry in urinary tract infection screening: Clinica Chimica Acta:2013 Vol 424: 23 September 90-95
    2. Shobha K.L, Sugandhi PRao: Prevalance of Antimicrobial resistance among Escherichia coli isolated from Urinary tract infection at Kasturba Medical Hospital, Manipal: The Antiseptics: June 2005: vol 102 No 6:316 - 318
    3. Guido Schmiemann, Eberhardt Knniehl, Klaus Gebhardt, Martha M Matejczyk, Eva Hummers Pradier: Diagnosis of Urinary tract infection ,A systematic review: Duetsches Arzteblatt International: May 2010:107(21): 361 – 367
    4. Konemann EW, Allen SD, Janda WW et al: .Koneman’s Color Atlas and textbook of diagnostic microbiology 6TH ed. 2006: (Lippin Cott Williams and Wilkins publications.
    5. Baron EJ, Pererson LR, Finegold SM (eds).Bailey and Scott’s Diagnostic Microbiology, 2003: 9th ed. Louis, USA: CV Mosby.
    6. P.Ayazi, A.Rahmani: Comparison of urine and gram stained smears of urine in diagnosis of urinary tract infections: Urology: 2010: 45-47D
    7. Bertt White: Diagnosis and treatment of urinary tract infections in children: American Fam Physician: 2001: Feb 15:83(4) 409-415.
    8. Waisman Y,Zeram E, Amirl, Mimouni M, The validity of the uriscreen test for early detection of urinary tract infection in children: Pediatrics:1999: Oct 104(4) :et41
    9. Marie E Westwood, Penny FwWhiteing, Julie Cooper. Ian S Watt, Jos Kleijnen: Further investigation of confirmed UTI in children under 5 years: a systematic review: BMC Pediatric: March 2005: 15(1)
    10. Kckhart, Lewander: Routine clinical analysis: Recent research in science and technology: 2010, 38-39.

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  • How to Cite

    K.L, S., Rao, A., K, S., & L, R. (2014). Comparison of screening tests with urine culture in urinary tract infection. International Journal of Medicine, 2(2), 81-83. https://doi.org/10.14419/ijm.v2i2.3607